Elucidation of the structural basis of transporter proteins involved in metal tolerance
Project/Area Number |
15K06972
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Research Category |
Grant-in-Aid for Scientific Research (C)
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Allocation Type | Multi-year Fund |
Section | 一般 |
Research Field |
Structural biochemistry
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Research Institution | Nara Institute of Science and Technology |
Principal Investigator |
TANAKA Yoshiki 奈良先端科学技術大学院大学, バイオサイエンス研究科, 助教 (10632333)
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Co-Investigator(Renkei-kenkyūsha) |
TSUKAZAKI Tomoya 奈良先端科学技術大学院大学, バイオサイエンス研究科, 准教授 (80436716)
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Research Collaborator |
IWAKI Shigehiro
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Project Period (FY) |
2015-04-01 – 2018-03-31
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Project Status |
Completed (Fiscal Year 2017)
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Budget Amount *help |
¥5,070,000 (Direct Cost: ¥3,900,000、Indirect Cost: ¥1,170,000)
Fiscal Year 2017: ¥1,820,000 (Direct Cost: ¥1,400,000、Indirect Cost: ¥420,000)
Fiscal Year 2016: ¥1,690,000 (Direct Cost: ¥1,300,000、Indirect Cost: ¥390,000)
Fiscal Year 2015: ¥1,560,000 (Direct Cost: ¥1,200,000、Indirect Cost: ¥360,000)
|
Keywords | タンパク質構造 / 膜タンパク質 / 構造解析 / 輸送体膜タンパク質 / 輸送体 / タンパク質 / イオン輸送 / X線結晶構造解析 / 構造生物学 |
Outline of Final Research Achievements |
We conducted a crystal structure analysis on the multidrug efflux transporter MATE, which is a transporter related to plant metal tolerance, and reported its structure. In the structure revealed, it became clear that some acidic amino acid side chains are concentrated in the inner pocket part. It was found that the aspect of the substrate binding region is different between the prokaryotic MATE which discharges hydrophobic substrates and the plant MATE which is related to emit cation such as aluminum.
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Report
(4 results)
Research Products
(13 results)
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[Journal Article] Tunnel Formation Inferred from the I-Form Structures of the Proton-Driven Protein Secretion Motor SecDF2017
Author(s)
Arata Furukawa, Kunihito Yoshikaie, Takaharu Mori, Hiroyuki Mori, Yusuke V. Morimoto, Yasunori Sugano, Shigehiro Iwaki, Tohru Minamino, Yuji Sugita, Yoshiki Tanaka, Tomoya Tsukazaki
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Journal Title
Cell Reports
Volume: 19
Issue: 5
Pages: 1-7
DOI
Related Report
Peer Reviewed / Open Access / Acknowledgement Compliant
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[Journal Article] BepA mediates productive transfer of substrate proteins to the β-barrel assembly machinery (BAM) complex.2017
Author(s)
Daimon, Y., Masui, C., Tanaka, Y., Shiota, T., Suzuki, T., Miyazaki, R., Sakurada, H., Lithgow, T., Dohmae, N., Mori, H., Tsukazaki, T., Narita, S. and Akiyama, Y.
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Journal Title
Mol. Microbiol.
Volume: 106
Issue: 5
Pages: 760-776
DOI
Related Report
Peer Reviewed / Open Access / Int'l Joint Research
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[Journal Article] Crystal structures of SecYEG in lipidic cubic phase elucidate a precise resting and a peptide-bound states2015
Author(s)
Yoshiki Tanaka, Yasunori Sugano, Mizuki Takemoto, Takaharu Mori, Arata Furukawa, Tsukasa Kusakizako, Kaoru Kumazaki, Ayako Kashima, Ryuichiro Ishitani, Yuji Sugita, Osamu Nureki and Tomoya Tsukazaki
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Journal Title
Cell Reports
Volume: 13
Pages: 15611568-15611568
NAID
Related Report
Peer Reviewed / Open Access / Acknowledgement Compliant
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[Presentation] Complete and peptide-bound structures of the Sec translocon2015
Author(s)
Yoshiki Tanaka, Yasunori Sugano, Mizuki Takemoto, Takaharu Mori, Arata Furukawa, Tsukasa Kusakizako, Kaoru Kumazaki, Ayako Kashima, Ryuichiro Ishitani, Yuji Sugita, Osamu Nureki and Tomoya Tsukazaki
Organizer
新学術領域研究「動的秩序と機能」第4回公開国際シンポジウム
Place of Presentation
九州大学西新プラザ(福岡県福岡市)
Year and Date
2015-11-22
Related Report
Int'l Joint Research
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[Presentation] Structures of Membrane Protein Insertase YidC2015
Author(s)
Tomoya Tsukazaki, Kaoru Kumazaki, Shinobu Chiba, Mizuki Takemoto, Arata Furukawa, Toshiki Kishimoto, Yasunori Sugano, Takaharu Mori, Yoshiki Tanaka, Yuji Sugita, Koreaki Ito, Ryuichiro Ishitani and Osamu Nureki
Organizer
Structural Biological Dynamics: From Molecules to Life with 60 trillion Cells
Place of Presentation
東京大学(東京都文京区)
Year and Date
2015-11-05
Related Report
Int'l Joint Research
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[Presentation] Cytoplasmic conformational transition of Sec translocon2015
Author(s)
Yoshiki Tanaka, Yasunori Sugano, Mizuki Takemoto, Takaharu Mori, Takamitsu Haruyama, Arata Furukawa, Tsukasa Kusakizako, Kaoru Kumazaki, Ayako Kashima, Ryuichiro Ishitani, Hiroki Konno, Yuji Sugita, Osamu Nureki and Tomoya Tsukazaki
Organizer
第53回日本生物物理学会年会
Place of Presentation
金沢大学(石川県金沢市)
Year and Date
2015-09-13
Related Report
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