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Cell wall peptidoglycan recognition/hydrolysis mechanisms of Clostridium perfringens lytic enzymes based on X-ray structures

Research Project

Project/Area Number 15K06973
Research Category

Grant-in-Aid for Scientific Research (C)

Allocation TypeMulti-year Fund
Section一般
Research Field Structural biochemistry
Research InstitutionKagawa University

Principal Investigator

Kamitori Shigehiro  香川大学, 総合生命科学研究センター, 教授 (00262246)

Co-Investigator(Kenkyū-buntansha) 吉田 裕美  香川大学, 総合生命科学研究センター, 准教授 (10313305)
Co-Investigator(Renkei-kenkyūsha) TAMAI Eiji  松山大学, 薬学部, 准教授 (40333512)
NOGUCHI Keiichi  東京農工大学, 学術研究支援総合センター, 准教授 (00251588)
Project Period (FY) 2015-04-01 – 2018-03-31
Project Status Completed (Fiscal Year 2017)
Budget Amount *help
¥5,070,000 (Direct Cost: ¥3,900,000、Indirect Cost: ¥1,170,000)
Fiscal Year 2017: ¥1,430,000 (Direct Cost: ¥1,100,000、Indirect Cost: ¥330,000)
Fiscal Year 2016: ¥1,560,000 (Direct Cost: ¥1,200,000、Indirect Cost: ¥360,000)
Fiscal Year 2015: ¥2,080,000 (Direct Cost: ¥1,600,000、Indirect Cost: ¥480,000)
KeywordsX線結晶構造解析 / 溶菌酵素 / グリコシドヒドロラーゼ / 細菌細胞壁 / ペプチドグリカン / ウェルシュ菌 / X線結晶解析 / SH3ドメイン / 糖鎖加水分解酵素 / ソーテース / グルコサミニダーゼ / ディフィシル菌
Outline of Final Research Achievements

Gram-positive bacteria possess a thick cell wall that surrounds their cytoplasmic membranes and provides physical protection. The bacterial cell wall is a mesh polymer of peptidoglycans, in which linear glycan backbones are cross-linked by species-specific peptide side chains. Autolysins can partially hydrolyze cell wall peptidoglycan into small sections to regulate cell separation/division and the growth-phase of bacteria. An X-ray structure of Clostridium perfringens autolysin catalytic domain was determined, giving new insights into the catalytic reaction mechanism for the hydrolysis of cell wall peptidoglycan. The pathogenesis and infectivity of Gram-positive bacteria are mediated by many surface proteins that are covalently attached to peptidoglycans of the cell wall. The covalent attachment of these proteins is catalyzed by sortases. X-ray structures of Clostridium perfringens sortase B and its inactive mutant form were determined.

Report

(4 results)
  • 2017 Annual Research Report   Final Research Report ( PDF )
  • 2016 Research-status Report
  • 2015 Research-status Report
  • Research Products

    (10 results)

All 2017 2016 2015 Other

All Journal Article (2 results) (of which Peer Reviewed: 2 results,  Acknowledgement Compliant: 1 results) Presentation (4 results) (of which Invited: 1 results) Book (1 results) Remarks (3 results)

  • [Journal Article] X-ray structure of Clostridium perfringens sortase B cysteine transpeptidase.2017

    • Author(s)
      Tamai E, Sekiya H, Maki J, Nariya H, Yoshida H, Kamitori S
    • Journal Title

      Biochem Biophys Res Commun.

      Volume: 493 Issue: 3 Pages: 1267-1272

    • DOI

      10.1016/j.bbrc.2017.09.144

    • Related Report
      2017 Annual Research Report
    • Peer Reviewed
  • [Journal Article] Structural and biochemical characterization of the Clostridium perfringens autolysin catalytic domain.2017

    • Author(s)
      Tamai E, Sekiya H, Goda E, Makihata N, Maki J, Yoshida H, Kamitori S.
    • Journal Title

      FEBS Lett.

      Volume: 591 Pages: 231-239

    • Related Report
      2016 Research-status Report
    • Peer Reviewed / Acknowledgement Compliant
  • [Presentation] ウェルシュ菌線毛タンパク質CppAのX線結晶解析2017

    • Author(s)
      神鳥 成弘,玉井 栄治,関谷 洋志,牧 純,成谷 宏文
    • Organizer
      第90回日本生化学会大会
    • Related Report
      2017 Annual Research Report
  • [Presentation] ウェルシュ菌Sortase BのX線結解析2016

    • Author(s)
      神鳥 成弘,吉田 裕美,玉井 栄治,関谷 洋志,牧 純,成谷 宏文
    • Organizer
      第89回日本生化学会大会
    • Place of Presentation
      仙台国際センター
    • Related Report
      2016 Research-status Report
  • [Presentation] ウェルシュ菌溶菌酵素オートライシン触媒ドメインのX線結晶解析2015

    • Author(s)
      吉田 裕美,神鳥 成弘,玉井 栄治,関谷 洋志,牧 純
    • Organizer
      第88回日本生化学会大会、第38回日本分子生物学会 合同開催
    • Place of Presentation
      神戸国際会議場
    • Year and Date
      2015-12-01
    • Related Report
      2015 Research-status Report
  • [Presentation] 今日から始める細菌タンパク質の構造機能解析2015

    • Author(s)
      玉井 栄治,神鳥 成弘
    • Organizer
      日本細菌学会中国・四国支部総会
    • Place of Presentation
      岡山大学
    • Year and Date
      2015-10-03
    • Related Report
      2015 Research-status Report
    • Invited
  • [Book] SH Domains: Structure, Mechanisms and Applications2015

    • Author(s)
      Kamitori, S. & Yoshida, H. (Kurochkina Ed.)
    • Total Pages
      19
    • Publisher
      Springer International Publishing AG
    • Related Report
      2015 Research-status Report
  • [Remarks] 科学研究費補助金(神鳥・吉田)

    • URL

      http://www.kms.ac.jp/~xraylab/report/kaken/index.htm

    • Related Report
      2017 Annual Research Report
  • [Remarks] 科学研究費補助金(神鳥・吉田)

    • URL

      http://www.med.kagawa-u.ac.jp/~xraylab/report/kaken/index.htm

    • Related Report
      2016 Research-status Report
  • [Remarks] 科学研究費補助金報告書(神鳥・吉田)

    • URL

      http://www.med.kagawa-u.ac.jp/~xraylab/report/kaken/index.htm

    • Related Report
      2015 Research-status Report

URL: 

Published: 2015-04-16   Modified: 2019-03-29  

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