Small leucine-rich repeat proteoglycans associated with mature insoluble elastin serve as binding sites for galectins
| Project/Area Number |
15K06974
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| Research Category |
Grant-in-Aid for Scientific Research (C)
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| Allocation Type | Multi-year Fund |
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| Section | 一般 |
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| Research Field |
Structural biochemistry
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| Research Institution | Kagawa University |
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Principal Investigator |
Nishi Nozomu 香川大学, 総合生命科学研究センター, 准教授 (10145047)
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| Co-Investigator(Renkei-kenkyūsha) |
NONAKA Yasuhiro 香川大学, 医学部, 助教 (50569217)
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| Project Period (FY) |
2015-04-01 – 2018-03-31
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| Project Status |
Completed (Fiscal Year 2017)
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| Budget Amount *help |
¥5,070,000 (Direct Cost: ¥3,900,000、Indirect Cost: ¥1,170,000)
Fiscal Year 2017: ¥1,040,000 (Direct Cost: ¥800,000、Indirect Cost: ¥240,000)
Fiscal Year 2016: ¥1,950,000 (Direct Cost: ¥1,500,000、Indirect Cost: ¥450,000)
Fiscal Year 2015: ¥2,080,000 (Direct Cost: ¥1,600,000、Indirect Cost: ¥480,000)
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| Keywords | galectin / elastin / oligosaccharide / SLRPs / lumican / mimecan / prolargin / fibromodulin / crosslink / glycan |
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| Outline of Final Research Achievements |
We found that mature insoluble elastin is capable of binding Gal-9 and other members of the human galectin family. Lectin blot analysis of a series of commercial water-soluble elastin preparations, PES-(A) ~ PES-(E), revealed that only PES-(E) contained substances recognized by Gal-9. Gal-9-interacting substances in PES-(E) were affinity-purified, digested with trypsin and then analyzed by reversed-phase HPLC. Peptide fragments derived from five members of the small leucine-rich repeat proteoglycan family, versican, lumican, osteoglycin/mimecan, prolargin and fibromodulin, were identified by N-terminal amino acid sequence analysis. The results indicate that Gal-9 and possibly other galectins recognize glycans attached to small leucine-rich repeat proteoglycans associated with insoluble elastin and also indicate the possibility that mature insoluble elastin serves as an extracellular reservoir for galectins.
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Report
(4 results)
Research Products
(11 results)
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[Journal Article] Cooperative interactions of oligosaccharide and peptide moieties of a glycopeptide derived from IgE with galectin-92016
Author(s)
Nakakita, S.-I., Itoh, A., Nakakita, Y., Nonaka, Y., Ogawa, T., Nakamura, T., Nishi, N.
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Journal Title
J. Biol. Chem.
Volume: 291
Issue: 2
Pages: 968-979
DOI
Related Report
Peer Reviewed / Open Access / Acknowledgement Compliant
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