| Budget Amount *help |
¥4,940,000 (Direct Cost: ¥3,800,000、Indirect Cost: ¥1,140,000)
Fiscal Year 2017: ¥1,560,000 (Direct Cost: ¥1,200,000、Indirect Cost: ¥360,000)
Fiscal Year 2016: ¥1,560,000 (Direct Cost: ¥1,200,000、Indirect Cost: ¥360,000)
Fiscal Year 2015: ¥1,820,000 (Direct Cost: ¥1,400,000、Indirect Cost: ¥420,000)
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| Outline of Final Research Achievements |
In this project, we addressed to develop new computational methods for solution NMR with sparse data or low signal to noise ratio. We successfully improved a Bayesian-assisted structure determination method and determined accurate structures of proteins in living cells and a protein which a conventional method could not converge. In addition, we could synthesize a new lanthanide-binding tag that stably incorporates with a protein even in reduction environment such as inside of cells. Using a method of data analysis developed in this project, we could obtain sufficiently large pseudo-contact shifts (PCSs) and long-range structural information of proteins in living cells.
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