Development of NMR data analysis methods for proten three-dimensional structure
Project/Area Number |
15K06979
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Research Category |
Grant-in-Aid for Scientific Research (C)
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Allocation Type | Multi-year Fund |
Section | 一般 |
Research Field |
Structural biochemistry
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Research Institution | Tokyo Metropolitan University |
Principal Investigator |
IKEYA TEPPEI 首都大学東京, 理工学研究科, 助教 (30457840)
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Co-Investigator(Renkei-kenkyūsha) |
ITO Yutaka 首都大学東京, 理工学研究科, 教授 (80261147)
GUENTERT Peter 首都大学東京, 理工学研究科, 客員教授 (20392110)
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Project Period (FY) |
2015-04-01 – 2018-03-31
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Project Status |
Completed (Fiscal Year 2017)
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Budget Amount *help |
¥4,940,000 (Direct Cost: ¥3,800,000、Indirect Cost: ¥1,140,000)
Fiscal Year 2017: ¥1,560,000 (Direct Cost: ¥1,200,000、Indirect Cost: ¥360,000)
Fiscal Year 2016: ¥1,560,000 (Direct Cost: ¥1,200,000、Indirect Cost: ¥360,000)
Fiscal Year 2015: ¥1,820,000 (Direct Cost: ¥1,400,000、Indirect Cost: ¥420,000)
|
Keywords | NMR立体構造計算 / ベイズ推定 / in-cell NMR / 高分子量蛋白質 / 蛋白質のダイナミクス / NMR / タンパク質立体構造決定 / 立体構造計算 / Bayes推定 / PCS / PRE |
Outline of Final Research Achievements |
In this project, we addressed to develop new computational methods for solution NMR with sparse data or low signal to noise ratio. We successfully improved a Bayesian-assisted structure determination method and determined accurate structures of proteins in living cells and a protein which a conventional method could not converge. In addition, we could synthesize a new lanthanide-binding tag that stably incorporates with a protein even in reduction environment such as inside of cells. Using a method of data analysis developed in this project, we could obtain sufficiently large pseudo-contact shifts (PCSs) and long-range structural information of proteins in living cells.
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Report
(4 results)
Research Products
(48 results)
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[Presentation] NMR PROTEIN STRUCTURE ANALYSIS IN LIVING CELLS2017
Author(s)
Teppei Ikeya, Peter Guentert, Yutaka Ito
Organizer
The 5th International Symposium on Dynamical Ordering of Biomolecular Systems for Creation of Integrated Functions
Place of Presentation
東京大学 (東京)
Year and Date
2017-01-21
Related Report
Int'l Joint Research
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[Presentation] NMR snapshots of a fluctuating ubiquitin structure2016
Author(s)
Soichiro Kitazawa, Takuro Wakamoto, Teppei Ikeya, Tomoshi Kameda, Maho Yagi-Utsumi, Koichi Kato, Christian Roumestand, Nicola J. Baxter, Mike P. Williamson, Ryo Kitahara
Organizer
EUROMAR 2016
Place of Presentation
Aarhus Concert Hall (Aarhus・Denmark)
Year and Date
2016-07-03
Related Report
Int'l Joint Research
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