| Budget Amount *help |
¥5,070,000 (Direct Cost: ¥3,900,000、Indirect Cost: ¥1,170,000)
Fiscal Year 2017: ¥1,690,000 (Direct Cost: ¥1,300,000、Indirect Cost: ¥390,000)
Fiscal Year 2016: ¥1,690,000 (Direct Cost: ¥1,300,000、Indirect Cost: ¥390,000)
Fiscal Year 2015: ¥1,690,000 (Direct Cost: ¥1,300,000、Indirect Cost: ¥390,000)
|
|---|
| Outline of Final Research Achievements |
To explore the effects of lipid environment on the function of sarcoplasmic reticulum Ca2+-ATPase, I tried to embed the Ca2+-ATPase into small lipid-bilayer (nanodiscs) with various lipid components. The Ca2+-ATPase was successfully embedded into the nanodisc without loss of its activities and the Ca2+-ATPase-embedded nanodiscs were utilized for various analyses. Subsequently, the contributions of lipid environments on the enzymatic activities were estimated quantitively by systematic analyses and could be distinguished between components of electrostatic- and steric-interactions. Furthermore, the results indicated that surface charges, but not particular lipid-head group, directly modulate the functions of Ca2+-ATPase. This finding is possibly important for not only Ca2+-ATPase but also for other functional membrane proteins.
|
