The molecular mechanisms of interactions between immune cells and its target antigens involved in the vertebrate tissue remodeling
| Project/Area Number |
15K06992
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| Research Category |
Grant-in-Aid for Scientific Research (C)
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| Allocation Type | Multi-year Fund |
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| Section | 一般 |
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| Research Field |
Functional biochemistry
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| Research Institution | Niigata University |
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Principal Investigator |
Izutsu Yumi 新潟大学, 自然科学系, 教授 (20301921)
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| Co-Investigator(Kenkyū-buntansha) |
伊藤 道彦 北里大学, 理学部, 准教授 (90240994)
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| Research Collaborator |
小林 遙香 , 大学院生
浅野 亮人 , 大学院生
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| Project Period (FY) |
2015-04-01 – 2018-03-31
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| Project Status |
Completed (Fiscal Year 2017)
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| Budget Amount *help |
¥4,940,000 (Direct Cost: ¥3,800,000、Indirect Cost: ¥1,140,000)
Fiscal Year 2017: ¥780,000 (Direct Cost: ¥600,000、Indirect Cost: ¥180,000)
Fiscal Year 2016: ¥1,170,000 (Direct Cost: ¥900,000、Indirect Cost: ¥270,000)
Fiscal Year 2015: ¥2,990,000 (Direct Cost: ¥2,300,000、Indirect Cost: ¥690,000)
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| Keywords | タンパク質 / 発現調節 / 発生・分化 / 組織・細胞 / 両生類 / アポトーシス / T細胞 / 免疫学 / 発現制御 / 細胞・組織 / 免疫 / アフリカツメガエル / 細胞死 / 蛋白質 |
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| Outline of Final Research Achievements |
We have previously reported that Ouro proteins and adult T cells are involved in the process of the tail degeneration during metamorphosis (Mukaigasa et al….. & Izutsu, 2009). We presented here the loss-of-function and rescue analyses to investigate whether or not the immune T cells are necessary for the tadpole’s tail regression via Ouro proteins during metamorphosis. To remove the T cells from the tadpoles, we used a monoclonal antibody against Xenopus T cell named XT-1. After XT-1 treatment with pre-metamorphic tadpoles, degeneration of Ouro-expressing tail tissues was significantly inhibited. To investigate T cell potency upon degeneration of the tadpole tail tissues, the rescue experiment was performed by transfer of T cells obtained from the J strain frogs into tadpoles, in which the T cells were depleted. It was succeeded in providing degenerated tails of a transgenic.
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Report
(4 results)
Research Products
(16 results)
