| Budget Amount *help |
¥5,200,000 (Direct Cost: ¥4,000,000、Indirect Cost: ¥1,200,000)
Fiscal Year 2017: ¥1,560,000 (Direct Cost: ¥1,200,000、Indirect Cost: ¥360,000)
Fiscal Year 2016: ¥1,560,000 (Direct Cost: ¥1,200,000、Indirect Cost: ¥360,000)
Fiscal Year 2015: ¥2,080,000 (Direct Cost: ¥1,600,000、Indirect Cost: ¥480,000)
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| Outline of Final Research Achievements |
Dipeptides are produced and degraded in mammalian cells during protein turnover and digestion to obtain amino acids required for nutritional demands. In addition, some kinds of dipeptides such as carnosine are synthesized from amino acids in specific organs and play some specific roles as a bioactive molecule. We have previously found that a metallopeptidase CN2 hydrolyses several kinds of dipeptides including carnosine. In this study, we have determined the substrate specificity of CN2, and shown that CN2-Mn2+ and CN2-Zn2+ complexes have different substrate specificity. When substrate specificity in cultured cells was examined, it was more similar to that of the CN2-Zn2+ complex observed in in vitro enzyme assay. These results demonstrate the role of CN2 in the final step of protein and peptide breakdown.
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