| Budget Amount *help |
¥5,070,000 (Direct Cost: ¥3,900,000、Indirect Cost: ¥1,170,000)
Fiscal Year 2017: ¥1,430,000 (Direct Cost: ¥1,100,000、Indirect Cost: ¥330,000)
Fiscal Year 2016: ¥1,560,000 (Direct Cost: ¥1,200,000、Indirect Cost: ¥360,000)
Fiscal Year 2015: ¥2,080,000 (Direct Cost: ¥1,600,000、Indirect Cost: ¥480,000)
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| Outline of Final Research Achievements |
Dynamin has been firstly identified as a microtubule-associated protein. However, the function of dynamin bound to microtubules is still unknown. This study aimed to elucidate the molecular mechanism of microtubule regulation by dynamin. It was revealed that depletion of dynamin stabilizes microtubules and the stabilized microtubules in dynamin-depleted cells are decreased by overexpression of GFP-dynamin-wild type or proline rich-deleted dynamin. On the other hand, overexpression of each GFP-dynamin mutant without GTPase, middle, plekstrin homology or GTPase effector did not decrease the stabilized microtubules. In addition, it was showed that overexpression of dynamin mutant capable of stabilizing microtubules increases the staining of EB1, one of microtubule plus-end tracking proteins, without changing the CLIP-170 or CLASP2. This study suggests that dynamin could regulate microtubules through its own GTPase activity and EB1.
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