| Budget Amount *help |
¥5,070,000 (Direct Cost: ¥3,900,000、Indirect Cost: ¥1,170,000)
Fiscal Year 2017: ¥1,690,000 (Direct Cost: ¥1,300,000、Indirect Cost: ¥390,000)
Fiscal Year 2016: ¥1,690,000 (Direct Cost: ¥1,300,000、Indirect Cost: ¥390,000)
Fiscal Year 2015: ¥1,690,000 (Direct Cost: ¥1,300,000、Indirect Cost: ¥390,000)
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| Outline of Final Research Achievements |
Main findings are as follows; (1) Excess DNA replication initiation factor Cdt1 inhibits MCM helicase activity in frog eggs extract. (2) Chicken DT40 TIPIN KO cells shows CTP (DNA topoisomerase I inhibitor) hypersensitivity. Under low dose CPT exposure, DNA double strand breaks are accumulated in TIPIN KO, leading to hyper checkpoint activation. (3) After UV irradiation, UV sensitivity and UV-induced hyper mutation of DT40 Polh single KO cells are almost completely suppressed by WRNIP1 gene knockout, suggesting that WRNIP1 acts upstream of Polh. (4) One of translation polymerases, PriPol is down-regulated by overproduction of WRNIP1. PriPol is the best candidate for error-free translesion polymerase on UV-damaged DNA in the absence of both WRNIP1 and Polh.
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