The elucidation of the mechanisms of ion selectivity and ion transport pathway of Mrp type Na+/H+ antiporter
| Project/Area Number |
15K07012
|
|---|
| Research Category |
Grant-in-Aid for Scientific Research (C)
|
| Allocation Type | Multi-year Fund |
|---|
| Section | 一般 |
|---|
| Research Field |
Functional biochemistry
|
|---|
| Research Institution | Toyo University |
|---|
Principal Investigator |
Ito Masairo 東洋大学, 生命科学部, 教授 (80297738)
|
|---|
| Project Period (FY) |
2015-04-01 – 2018-03-31
|
| Project Status |
Completed (Fiscal Year 2017)
|
| Budget Amount *help |
¥5,070,000 (Direct Cost: ¥3,900,000、Indirect Cost: ¥1,170,000)
Fiscal Year 2017: ¥1,430,000 (Direct Cost: ¥1,100,000、Indirect Cost: ¥330,000)
Fiscal Year 2016: ¥1,430,000 (Direct Cost: ¥1,100,000、Indirect Cost: ¥330,000)
Fiscal Year 2015: ¥2,210,000 (Direct Cost: ¥1,700,000、Indirect Cost: ¥510,000)
|
|---|
| Keywords | アンチポーター / Mrp / イオン選択性 / 好アルカリ性細菌 / ｐHホメオスタシス / Bacillus / カルシウムイオン / ナトリウムイオン / pHホメオスタシス / カリウムイオン |
|---|
| Outline of Final Research Achievements |
In this study, clarification of the ion transport route of Mrp type Na+/H+ antiporter was carried out. It has been reported that Ca2+ is transported in addition to Na+ and K+ among homologous proteins of this enzyme. Therefore, we attempted to elucidate the purpose of this research by using three different Mrp homologs of cations to be transported. As a result, it was revealed that the coupling cation transport route of this enzyme was formed at the fifth transmembrane segment of MrpA and the twelfth transmembrane segment of MrpD which are the interface between the two subunits.
|
Report
(4 results)
Research Products
(9 results)
