| Budget Amount *help |
¥5,070,000 (Direct Cost: ¥3,900,000、Indirect Cost: ¥1,170,000)
Fiscal Year 2017: ¥1,690,000 (Direct Cost: ¥1,300,000、Indirect Cost: ¥390,000)
Fiscal Year 2016: ¥1,690,000 (Direct Cost: ¥1,300,000、Indirect Cost: ¥390,000)
Fiscal Year 2015: ¥1,690,000 (Direct Cost: ¥1,300,000、Indirect Cost: ¥390,000)
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| Outline of Final Research Achievements |
UDP-apiose, a donor substrate of apiosyltransferases, is known to a labile due to the intramolecular self-cyclization, resulting the formation of a-D-apio-D-furanosyl 1:2-cyclic phosphate. Its half-life is 32 seconds at at pH 8.0 and 25 C. We found that the bulky caions, especially tertiary amines, effectively suppressed the degradation of UDP-apiose. Consequently, a half-life of UDP-apiose was prolonged to 38.5 hours at pH 6.0 and 25C using triethylamine as a counter ion. Using this isolated UDP-apiose, we identified a gene encoding pectin rhamnogalacturonan II: apiosyltransferase.
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