Identification of apiosyltransferase involved in biosynthesis of pectin in plant cell wall
Project/Area Number |
15K07016
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Research Category |
Grant-in-Aid for Scientific Research (C)
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Allocation Type | Multi-year Fund |
Section | 一般 |
Research Field |
Functional biochemistry
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Research Institution | Ritsumeikan University |
Principal Investigator |
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Project Period (FY) |
2015-04-01 – 2018-03-31
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Project Status |
Completed (Fiscal Year 2017)
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Budget Amount *help |
¥5,070,000 (Direct Cost: ¥3,900,000、Indirect Cost: ¥1,170,000)
Fiscal Year 2017: ¥1,690,000 (Direct Cost: ¥1,300,000、Indirect Cost: ¥390,000)
Fiscal Year 2016: ¥1,690,000 (Direct Cost: ¥1,300,000、Indirect Cost: ¥390,000)
Fiscal Year 2015: ¥1,690,000 (Direct Cost: ¥1,300,000、Indirect Cost: ¥390,000)
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Keywords | 植物 / 細胞壁 / 多糖 / ペクチン / 糖転移酵素 / 糖ヌクレオチド / アピオース / 酵素 / 糖鎖 |
Outline of Final Research Achievements |
UDP-apiose, a donor substrate of apiosyltransferases, is known to a labile due to the intramolecular self-cyclization, resulting the formation of a-D-apio-D-furanosyl 1:2-cyclic phosphate. Its half-life is 32 seconds at at pH 8.0 and 25 C. We found that the bulky caions, especially tertiary amines, effectively suppressed the degradation of UDP-apiose. Consequently, a half-life of UDP-apiose was prolonged to 38.5 hours at pH 6.0 and 25C using triethylamine as a counter ion. Using this isolated UDP-apiose, we identified a gene encoding pectin rhamnogalacturonan II: apiosyltransferase.
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Report
(4 results)
Research Products
(42 results)
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[Journal Article] Biochemical characterization of rhamnosyltransferase involved in biosynthesis of pectic rhamnogalacturonan I in plant cell wall2017
Author(s)
Uehara Y, Tamura S, Maki Y, Yagyu K, Mizoguchi T, Tamiaki H, Imai T, Ishii T, Ohashi T, Fujiyama K, Ishimizu T
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Journal Title
Biochemical and Biophysical Research Communications
Volume: 486
Issue: 1
Pages: 130-136
DOI
Related Report
Peer Reviewed
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[Journal Article] Molecular characterization of tomato α1,3/4-fucosidase, a member of glycosyl hydrolase family 29, involved in degradation of plant complex type N-glycans.2017
Author(s)
Rahman, M.D., Maeda, M., Itano, S., Hossain, M.A., Ishimizu, T., Kimura, Y.
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Journal Title
J. Biochem.
Volume: 161
Pages: 421-432
DOI
NAID
Related Report
Peer Reviewed / Open Access / Int'l Joint Research
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[Presentation] Discovery and biochemical characterization of rhamnosyltransferase involved in pectin biosynthesis in Arabidopsis thariana2017
Author(s)
Yuto Takenaka, Kohei Kato, Mari Ogawa-Ohnishi, Kana Tsuruhama, Hiroyuki Kajiura, Kenta Yagyu, Atsushi Takeda, Tadashi Kunieda, Ikuko Hara-Nishimura, Takeshi Kuroha, Kazuhiko Nishitani, Yoshikatsu Matsubayashi, and Takeshi Ishimizu
Organizer
Taiwan-Japan Plant Biology 2017
Related Report
Int'l Joint Research
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[Presentation] Degradation pathway of plant complex-type N-glycans: Identification and characterization of α1,3-fucosidase2016
Author(s)
Kato, S., Hayashi, M., Kitagawa, M., Maeda, M., Kimura, Y., Igarashi, K., Kasahara, M. and Ishimizu, T.
Organizer
28th International Carbohydrate Symposium
Place of Presentation
ニューオリンズ(アメリカ)
Year and Date
2016-07-19
Related Report
Int'l Joint Research
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