Nucleolar stress sensing in the nucleolus
| Project/Area Number |
15K07020
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| Research Category |
Grant-in-Aid for Scientific Research (C)
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| Allocation Type | Multi-year Fund |
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| Section | 一般 |
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| Research Field |
Functional biochemistry
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| Research Institution | Kobe University (2016-2017)
National Institute of Infectious Diseases (2015) |
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Principal Investigator |
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| Project Period (FY) |
2015-04-01 – 2018-03-31
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| Project Status |
Completed (Fiscal Year 2017)
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| Budget Amount *help |
¥5,070,000 (Direct Cost: ¥3,900,000、Indirect Cost: ¥1,170,000)
Fiscal Year 2017: ¥1,560,000 (Direct Cost: ¥1,200,000、Indirect Cost: ¥360,000)
Fiscal Year 2016: ¥1,430,000 (Direct Cost: ¥1,100,000、Indirect Cost: ¥330,000)
Fiscal Year 2015: ¥2,080,000 (Direct Cost: ¥1,600,000、Indirect Cost: ¥480,000)
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| Keywords | シグナル伝達 / 核小体ストレス / ＰＩＣＴ１ / PICT1 |
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| Outline of Final Research Achievements |
The nucleolus is a signaling center for nucleolar stress, which is induced by impaired ribosome biogenesis. PICT1 is known to play critical roles in the nucleolar stress response. In this study, I performed siRNA-mediated gene silencing, affinity pulldown, and in vitro PICT1 degradation assay to identify a stabilizing factor(s) which binds to and stabilizes PICT1 protein. I also conducted immunoprecipitation assay to analyze nucleolar stress-induced dissociation of PICT1 and candidate stabilizing factor(s). As the result, I successful found that RPX directly bound to PICT1 and repressed PICT1 degradation by 20S proteasome in vitro. In addition, immunoprecipitation assay showed that the nucleolar stress significantly decreased PICT1-RPX interaction, suggesting RPX is an upstream factor which regulates PICT1 stability and the nucleolar stress response.
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Report
(4 results)
Research Products
(12 results)
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[Journal Article] Loss of Mob1a/b in mice results in chondrodysplasia due to YAP1/TAZ-TEAD-dependent repression of SOX9.2018
Author(s)
Goto H, Nishio M, To Y, Oishi T, Miyachi Y, Maehama T, Nishina H, Akiyama H, Mak TW, Makii Y, Saito T, Yasoda A, Tsumaki N, Suzuki A.
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Journal Title
Development
Volume: 145
Issue: 6
Pages: 159244-159244
DOI
Related Report
Peer Reviewed / Open Access / Int'l Joint Research
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[Journal Article] MOB1-YAP1/TAZ-NKX2.1 axis controls bronchioalveolar cell differentiation, adhesion and tumour formation. Oncogene2017
Author(s)
Otsubo K, Goto H, Nishio M, Kawamura K, Yanagi S, Nishie W, Sasaki T, Maehama T, Nishina H, Mimori K, Nakano T, Shimizu H, Mak TW, Nakao K, Nakanishi Y, Suzuki A
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Journal Title
Oncogene
Volume: 印刷中
Issue: 29
Pages: 4201-4211
DOI
Related Report
Peer Reviewed / Open Access / Int'l Joint Research / Acknowledgement Compliant
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