• Search Research Projects
  • Search Researchers
  • How to Use
  1. Back to previous page

Nucleolar stress sensing in the nucleolus

Research Project

Project/Area Number 15K07020
Research Category

Grant-in-Aid for Scientific Research (C)

Allocation TypeMulti-year Fund
Section一般
Research Field Functional biochemistry
Research InstitutionKobe University (2016-2017)
National Institute of Infectious Diseases (2015)

Principal Investigator

Maehama Tomohiko  神戸大学, 医学研究科, 准教授 (40322755)

Project Period (FY) 2015-04-01 – 2018-03-31
Project Status Completed (Fiscal Year 2017)
Budget Amount *help
¥5,070,000 (Direct Cost: ¥3,900,000、Indirect Cost: ¥1,170,000)
Fiscal Year 2017: ¥1,560,000 (Direct Cost: ¥1,200,000、Indirect Cost: ¥360,000)
Fiscal Year 2016: ¥1,430,000 (Direct Cost: ¥1,100,000、Indirect Cost: ¥330,000)
Fiscal Year 2015: ¥2,080,000 (Direct Cost: ¥1,600,000、Indirect Cost: ¥480,000)
Keywordsシグナル伝達 / 核小体ストレス / PICT1 / PICT1
Outline of Final Research Achievements

The nucleolus is a signaling center for nucleolar stress, which is induced by impaired ribosome biogenesis. PICT1 is known to play critical roles in the nucleolar stress response. In this study, I performed siRNA-mediated gene silencing, affinity pulldown, and in vitro PICT1 degradation assay to identify a stabilizing factor(s) which binds to and stabilizes PICT1 protein. I also conducted immunoprecipitation assay to analyze nucleolar stress-induced dissociation of PICT1 and candidate stabilizing factor(s). As the result, I successful found that RPX directly bound to PICT1 and repressed PICT1 degradation by 20S proteasome in vitro. In addition, immunoprecipitation assay showed that the nucleolar stress significantly decreased PICT1-RPX interaction, suggesting RPX is an upstream factor which regulates PICT1 stability and the nucleolar stress response.

Report

(4 results)
  • 2017 Annual Research Report   Final Research Report ( PDF )
  • 2016 Research-status Report
  • 2015 Research-status Report
  • Research Products

    (12 results)

All 2018 2017 2016 2015

All Journal Article (6 results) (of which Int'l Joint Research: 2 results,  Peer Reviewed: 4 results,  Open Access: 4 results,  Acknowledgement Compliant: 1 results) Presentation (6 results) (of which Int'l Joint Research: 4 results)

  • [Journal Article] Loss of Mob1a/b in mice results in chondrodysplasia due to YAP1/TAZ-TEAD-dependent repression of SOX9.2018

    • Author(s)
      Goto H, Nishio M, To Y, Oishi T, Miyachi Y, Maehama T, Nishina H, Akiyama H, Mak TW, Makii Y, Saito T, Yasoda A, Tsumaki N, Suzuki A.
    • Journal Title

      Development

      Volume: 145 Issue: 6 Pages: 159244-159244

    • DOI

      10.1242/dev.159244

    • Related Report
      2017 Annual Research Report
    • Peer Reviewed / Open Access / Int'l Joint Research
  • [Journal Article] MOB1-YAP1/TAZ-NKX2.1 axis controls bronchioalveolar cell differentiation, adhesion and tumour formation. Oncogene2017

    • Author(s)
      Otsubo K, Goto H, Nishio M, Kawamura K, Yanagi S, Nishie W, Sasaki T, Maehama T, Nishina H, Mimori K, Nakano T, Shimizu H, Mak TW, Nakao K, Nakanishi Y, Suzuki A
    • Journal Title

      Oncogene

      Volume: 印刷中 Issue: 29 Pages: 4201-4211

    • DOI

      10.1038/onc.2017.58

    • Related Report
      2017 Annual Research Report 2016 Research-status Report
    • Peer Reviewed / Open Access / Int'l Joint Research / Acknowledgement Compliant
  • [Journal Article] Hippo vs. Crab: tissue-specific functions of the mammalian Hippo pathway2017

    • Author(s)
      M. Nishio, T. Maehama, H. Goto, K. Nakatani, W. Kato, H. Omori, Y. Miyachi, H. Togashi, Y. Shimono and A. Suzuki
    • Journal Title

      Genes to Cells

      Volume: 22 Issue: 1 Pages: 6-31

    • DOI

      10.1111/gtc.12461

    • Related Report
      2016 Research-status Report
    • Peer Reviewed / Open Access
  • [Journal Article] Targeting the Hippo signalling pathway for cancer treatment2016

    • Author(s)
      K. Nakatani, T. Maehama, M. Nishio, H. Goto, W. Kato, H. Omori, Y. Miyachi, H. Togashi, Y. Shimono and A. Suzuki
    • Journal Title

      J. Biochem.

      Volume: 161 Pages: 237-244

    • DOI

      10.1093/jb/mvw074

    • NAID

      40021162008

    • Related Report
      2016 Research-status Report
    • Peer Reviewed / Open Access
  • [Journal Article] PTEN:脂質ホスファターゼとしての機能と発がんへの関与2015

    • Author(s)
      前濱朝彦、鈴木聡
    • Journal Title

      実験医学増刊

      Volume: 15 Pages: 157-163

    • Related Report
      2015 Research-status Report
  • [Journal Article] PTEN2015

    • Author(s)
      前濱朝彦、鈴木聡
    • Journal Title

      日本臨牀増刊号

      Volume: 73 Pages: 285-290

    • Related Report
      2015 Research-status Report
  • [Presentation] Role of Hippo pathway in vivo2017

    • Author(s)
      Nishio M, Maehama T, Togashi H, et al.
    • Organizer
      神戸大学・ワシントン大学・オスロ大学国際合同シンポジウム
    • Place of Presentation
      神戸大学(兵庫県神戸市)
    • Year and Date
      2017-03-13
    • Related Report
      2016 Research-status Report
    • Int'l Joint Research
  • [Presentation] Hippo signaling pathway affects cell competion in mammalian cells via cell-ECM binding and anchorage-dependent cell proliferation2017

    • Author(s)
      Miki Nishio, Tomohiko Maehama, Akira Suzuki
    • Organizer
      3rd International Symposium on Cell Competition
    • Related Report
      2017 Annual Research Report
    • Int'l Joint Research
  • [Presentation] Role of MOB1A/1B in salivary gland2017

    • Author(s)
      Yosuke Miyachi, Miki Nishio, Hiroki Goto, Tomohiko Maehama, Akira Suzuki
    • Organizer
      EMBO workshop ‘The Hippo pathway across species and disciplines’
    • Related Report
      2017 Annual Research Report
    • Int'l Joint Research
  • [Presentation] がん制御因子PICT1の安定化機構2017

    • Author(s)
      前濱朝彦、宮地洋佑、西尾美希、鈴木聡
    • Organizer
      2017年度生命科学系学会合同年次大会ConBio
    • Related Report
      2017 Annual Research Report
  • [Presentation] Regulation of nucleolar stress response and tumorigenesis by PICT12017

    • Author(s)
      Tomohiko Maehama, Miki Nishio, Yosuke Miyachi, Akira Suzuki
    • Organizer
      第2回 神大・ワシントン大・オスロ大 国際合同シンポジウム
    • Related Report
      2017 Annual Research Report
    • Int'l Joint Research
  • [Presentation] 遺伝子改変動物を利用した、形態形成におけるHippo経路の機能解析2016

    • Author(s)
      田中雅人、後藤裕樹、前濱朝彦、他
    • Organizer
      第48回日本臨床分子形態学会ワークショップⅢ
    • Place of Presentation
      くまもと県民交流館パレア(熊本県熊本市)
    • Year and Date
      2016-09-23
    • Related Report
      2016 Research-status Report

URL: 

Published: 2015-04-16   Modified: 2019-03-29  

Information User Guide FAQ News Terms of Use Attribution of KAKENHI

Powered by NII kakenhi