| Budget Amount *help |
¥5,070,000 (Direct Cost: ¥3,900,000、Indirect Cost: ¥1,170,000)
Fiscal Year 2017: ¥1,560,000 (Direct Cost: ¥1,200,000、Indirect Cost: ¥360,000)
Fiscal Year 2016: ¥1,430,000 (Direct Cost: ¥1,100,000、Indirect Cost: ¥330,000)
Fiscal Year 2015: ¥2,080,000 (Direct Cost: ¥1,600,000、Indirect Cost: ¥480,000)
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| Outline of Final Research Achievements |
The nucleolus is a signaling center for nucleolar stress, which is induced by impaired ribosome biogenesis. PICT1 is known to play critical roles in the nucleolar stress response. In this study, I performed siRNA-mediated gene silencing, affinity pulldown, and in vitro PICT1 degradation assay to identify a stabilizing factor(s) which binds to and stabilizes PICT1 protein. I also conducted immunoprecipitation assay to analyze nucleolar stress-induced dissociation of PICT1 and candidate stabilizing factor(s). As the result, I successful found that RPX directly bound to PICT1 and repressed PICT1 degradation by 20S proteasome in vitro. In addition, immunoprecipitation assay showed that the nucleolar stress significantly decreased PICT1-RPX interaction, suggesting RPX is an upstream factor which regulates PICT1 stability and the nucleolar stress response.
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