| Budget Amount *help |
¥4,550,000 (Direct Cost: ¥3,500,000、Indirect Cost: ¥1,050,000)
Fiscal Year 2017: ¥1,560,000 (Direct Cost: ¥1,200,000、Indirect Cost: ¥360,000)
Fiscal Year 2016: ¥1,430,000 (Direct Cost: ¥1,100,000、Indirect Cost: ¥330,000)
Fiscal Year 2015: ¥1,560,000 (Direct Cost: ¥1,200,000、Indirect Cost: ¥360,000)
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| Outline of Final Research Achievements |
We made the minimal motor domain constructs for plus-ended kinesins, minus-ended kinesins and kinesin-1 - kinesin-14 chimeras. Kinesin monomers were engineered such that they could be anchored via either their N- or C-termini, leaving the opposite terminal regions mechanically disconnected from the surface. We find that the conserved catalytic motor core of minus-end-directed motor kinesin-14 has intrinsic plus-end directionality and that the proper interaction of the C-terminal neck-helix region and the N-terminal neck-mimic region are required to achieve minus-end directionality.
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