| Project/Area Number |
15K07025
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| Research Category |
Grant-in-Aid for Scientific Research (C)
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| Allocation Type | Multi-year Fund |
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| Section | 一般 |
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| Research Field |
Biophysics
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| Research Institution | Kanazawa Institute of Technology |
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Principal Investigator |
Tatsumi Hitoshi 金沢工業大学, バイオ・化学部, 教授 (20171720)
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| Co-Investigator(Kenkyū-buntansha) |
早川 公英 名古屋大学, 医学(系)研究科(研究院), その他 (60467280)
飯田 秀利 東京学芸大学, 教育学部, 名誉教授 (70124435)
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| Project Period (FY) |
2015-04-01 – 2018-03-31
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| Project Status |
Completed (Fiscal Year 2017)
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| Budget Amount *help |
¥4,940,000 (Direct Cost: ¥3,800,000、Indirect Cost: ¥1,140,000)
Fiscal Year 2017: ¥1,170,000 (Direct Cost: ¥900,000、Indirect Cost: ¥270,000)
Fiscal Year 2016: ¥910,000 (Direct Cost: ¥700,000、Indirect Cost: ¥210,000)
Fiscal Year 2015: ¥2,860,000 (Direct Cost: ¥2,200,000、Indirect Cost: ¥660,000)
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| Keywords | アクチン線維 / AIP1 / cofilin / 切断 / 一分子計測 / 近接場光 / キネティクス / 細胞運動 / actin / アクチン / コフィリン / 一分子 / イメージング / 張力 |
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| Outline of Final Research Achievements |
Rearrangement of actin filaments by polymerization, depolymerization, and severing, is important for many cellular functions. Cofilin and actin-interacting protein 1 (AIP1) are evolutionally conserved proteins. They cooperatively sever actin filaments. However, little is known about the biophysical basis of the actin-filament severing by cofilin and AIP1 proteins. We performed single molecule kinetics analyses of fluorescently labeled AIP1 during the severing process of cofilin-decorated actin filaments that was fluorescently labeled with a different color chromophore. Our results showed that binding of a single AIP molecule was sufficient to enhance filament severing and may follow the MM type kinetics.
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