| Budget Amount *help |
¥5,070,000 (Direct Cost: ¥3,900,000、Indirect Cost: ¥1,170,000)
Fiscal Year 2017: ¥1,560,000 (Direct Cost: ¥1,200,000、Indirect Cost: ¥360,000)
Fiscal Year 2016: ¥1,560,000 (Direct Cost: ¥1,200,000、Indirect Cost: ¥360,000)
Fiscal Year 2015: ¥1,950,000 (Direct Cost: ¥1,500,000、Indirect Cost: ¥450,000)
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| Outline of Final Research Achievements |
We developed new analytical method which can characterize the structure of membrane-bound protein using vacuum-ultraviolet circular-dichroism (VUVCD) and linear-dichroism (LD) spectroscopy. This VUVCD-LD method uses the flow-LD measurement system equipped with the microchannel type flow cell and liquid pump, and can analyze the orientation of secondary structures at the local region such as membrane binding site of protein. This system was applied to the structural analysis of alpha1-acid glycoprotein (AGP) and myelin-basic protein (MBP) in the membrane. It is known that AGP transports a drug into membrane and MBP relates to the myelination in nerve cell. The secondary structures of AGP and MBP were determined by VUVCD-LD method in the presecne of various liposomes, revealing that the drug-binding regions of AGP directly interacted with membrane and that the driving force of MBP-membrane binding was electrostatic interaction.
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