• Search Research Projects
  • Search Researchers
  • How to Use
  1. Back to previous page

Structural basis of energy transduction mechanism in the respiratory terminal enzymes.

Research Project

Project/Area Number 15K07029
Research Category

Grant-in-Aid for Scientific Research (C)

Allocation TypeMulti-year Fund
Section一般
Research Field Biophysics
Research InstitutionUniversity of Hyogo

Principal Investigator

Muramoto Kazumasa  兵庫県立大学, 生命理学研究科, 准教授 (50305679)

Project Period (FY) 2015-04-01 – 2018-03-31
Project Status Completed (Fiscal Year 2017)
Budget Amount *help
¥4,940,000 (Direct Cost: ¥3,800,000、Indirect Cost: ¥1,140,000)
Fiscal Year 2017: ¥1,560,000 (Direct Cost: ¥1,200,000、Indirect Cost: ¥360,000)
Fiscal Year 2016: ¥1,560,000 (Direct Cost: ¥1,200,000、Indirect Cost: ¥360,000)
Fiscal Year 2015: ¥1,820,000 (Direct Cost: ¥1,400,000、Indirect Cost: ¥420,000)
Keywords生物物理 / ナノバイオ / 蛋白質 / 酵素 / 呼吸鎖 / 生体エネルギー変換 / X線結晶構造解析 / 酵素反応速度論的解析
Outline of Final Research Achievements

Respiratory chains in mitochondrial and bacterial membrane transfer hydrogen ions (protons) by using energy released from the redox reaction (electron transfer), and efficiently generates energy available for the life. To understand the energy transduction mechanism, I studied molecular structure and function of the respiratory terminal enzymes. The results showed that structural property of the catalytic site and the proton transfer pathway, and functional property of the electron transfer between the enzyme and its electron donor.

Report

(4 results)
  • 2017 Annual Research Report   Final Research Report ( PDF )
  • 2016 Research-status Report
  • 2015 Research-status Report
  • Research Products

    (25 results)

All 2018 2017 2016 2015 Other

All Int'l Joint Research (2 results) Journal Article (3 results) (of which Int'l Joint Research: 1 results,  Peer Reviewed: 3 results,  Open Access: 1 results,  Acknowledgement Compliant: 1 results) Presentation (20 results) (of which Int'l Joint Research: 4 results)

  • [Int'l Joint Research] Stockholm University(Sweden)

    • Related Report
      2017 Annual Research Report
  • [Int'l Joint Research] University of Liverpool(United Kingdom)

    • Related Report
      2017 Annual Research Report
  • [Journal Article] Characterization of the Quinol-dependent Nitric Oxide Reductase from the Pathogen Neisseria meningitidis, an Electrogenic Enzyme2018

    • Author(s)
      N. Gonska, D. R. Young, R. Yuki, T. Okamoto, T. Hisano, S. V. Antonyuk, S. S. Hasnain, K. Muramoto, Y. Shiro, T. Tosha, P. Adelroth
    • Journal Title

      Sci. Rep.

      Volume: 8: 3637 Issue: 1 Pages: 3637-3637

    • DOI

      10.1038/s41598-018-21804-0

    • Related Report
      2017 Annual Research Report
    • Peer Reviewed / Open Access / Int'l Joint Research
  • [Journal Article] The Mg2+-containing Water Cluster of Mammalian Cytochrome c Oxidase Collects Four Pumping Proton Equivalents in Each Catalytic Cycle2016

    • Author(s)
      Yano N, Muramoto K, Shimada A, Takemura S, Baba J, Fujisawa H, Mochizuki M, Shinzawa-Itoh K, Yamashita E, Tsukihara T, Yoshikawa S.
    • Journal Title

      J. Biol. Chem.

      Volume: 291 Issue: 46 Pages: 23882-23894

    • DOI

      10.1074/jbc.m115.711770

    • Related Report
      2016 Research-status Report
    • Peer Reviewed / Acknowledgement Compliant
  • [Journal Article] X-ray structure of cyanide-bound bovine heart cytochrome c oxidase in the fully oxidized state at 2.0 angstrom resolution.2015

    • Author(s)
      Yano N, Muramoto K, Mochizuki M, Shinzawa-Itoh K, Yamashita E, Yoshikawa S, Tsukihara
    • Journal Title

      Acta Cryst. F

      Volume: 71 Issue: 6 Pages: 726-730

    • DOI

      10.1107/s2053230x15007025

    • Related Report
      2015 Research-status Report
    • Peer Reviewed
  • [Presentation] 髄膜炎菌由来一酸化窒素還元酵素の結晶化と速度論的解析2017

    • Author(s)
      岡本拓也、Arif Jamali、村本和優、當舎武彦、城宜嗣
    • Organizer
      日本生体エネルギー研究会第42回討論会
    • Related Report
      2017 Annual Research Report
  • [Presentation] Crystallization of quinol-dependent Nitric oxide reductase from Neisseria Meningitis.2017

    • Author(s)
      Arif M. M. Jamali, Kazumasa Muramoto,Takehiko Tosha ,Yoshitsugu Shiro
    • Organizer
      University of Hyogo Leading Program International Symposium
    • Related Report
      2017 Annual Research Report
    • Int'l Joint Research
  • [Presentation] pKa analysis of the proton transfer pathway in respiratory A-type heme-copper oxygen reductase.2017

    • Author(s)
      村本和優
    • Organizer
      日本生物物理学会第55回年会
    • Related Report
      2017 Annual Research Report
  • [Presentation] 緑膿菌一酸化窒素還元酵素によるシトクロムc551およびアズリン酸化反応の速度論的解析2016

    • Author(s)
      村本和優、大畠海人、當舎武彦、城宜嗣
    • Organizer
      日本生体エネルギー研究会第42回討論会
    • Place of Presentation
      名古屋工業大学(名古屋市)
    • Year and Date
      2016-12-19
    • Related Report
      2016 Research-status Report
  • [Presentation] 髄膜炎菌由来一酸化窒素還元酵の活性測定と結晶化2016

    • Author(s)
      結城力、岡本拓也、David Young、村本和優、當舎武彦、城宜嗣
    • Organizer
      日本生体エネルギー研究会第42回討論会
    • Place of Presentation
      名古屋工業大学(名古屋市)
    • Year and Date
      2016-12-19
    • Related Report
      2016 Research-status Report
  • [Presentation] Structural analysis of the proton transfer pathway in respiratory heme-copper oxygen reductase superfamily.2016

    • Author(s)
      村本和優
    • Organizer
      日本生物物理学会第54回年会
    • Place of Presentation
      つくば国際会議場(つくば市)
    • Year and Date
      2016-11-25
    • Related Report
      2016 Research-status Report
  • [Presentation] Structural and functional characterization of nanodisc-reconstituted cytochrome cbb3 oxidase from Vibrio cholerae.2016

    • Author(s)
      Inoue M, Shibata A, Imai M, Uchida T, Muramoto K, Shiro Y, Yoshikawa S, Ishimori K.
    • Organizer
      日本生物物理学会第54回年会
    • Place of Presentation
      つくば国際会議場(つくば市)
    • Year and Date
      2016-11-25
    • Related Report
      2016 Research-status Report
  • [Presentation] High-resolution crystal structure of cytochrome c oxidase reveals the mechanism of highly efficient proton pumping.2016

    • Author(s)
      Shimada A, Yano N, Muramoto K, Yamashita E, Shinzawa-Itoh K, Tsukihara T, Yoshikawa S.
    • Organizer
      日本生物物理学会第54回年会
    • Place of Presentation
      つくば国際会議場(つくば市)
    • Year and Date
      2016-11-25
    • Related Report
      2016 Research-status Report
  • [Presentation] Detailed crystal structural studies of bovine cytochrome oxidase to elucidate the coupling mechanism of dioxygen reduction and proton pump.2016

    • Author(s)
      月原冨武、島田敦広、矢野直峰、村本和優、新澤・伊藤恭子、山下栄樹、吉川信也
    • Organizer
      本生物物理学会第54回年会
    • Place of Presentation
      つくば国際会議場(つくば市)
    • Year and Date
      2016-11-25
    • Related Report
      2016 Research-status Report
  • [Presentation] The Mg2+-containing water cluster of mammalian cytochrome c oxidase collects four pumping proton equivalents in each catalytic cycle.2016

    • Author(s)
      Yoshikawa S, Yano N, Muramoto K, Shimada A, Baba J, Mochizuki M, Shinzawa-Itoh K, Yamashita E, Tsukihara T.
    • Organizer
      The 19th European Bioenergetics Conference
    • Place of Presentation
      Riva del Garda (Italy)
    • Year and Date
      2016-07-02
    • Related Report
      2016 Research-status Report
    • Int'l Joint Research
  • [Presentation] 髄膜炎菌由来一酸化窒素還元酵素の精製と結晶化2016

    • Author(s)
      結城力、岡本拓也、David Young、村本和優、當舎武彦、城宜嗣
    • Organizer
      第16回日本蛋白質科学会年会
    • Place of Presentation
      福岡国際会議場(福岡市)
    • Year and Date
      2016-06-07
    • Related Report
      2016 Research-status Report
  • [Presentation] チトクロム酸化酵素の様々な反応中間体及び反応中間体類似物の構造から提唱されるプロトンポンプ機構2016

    • Author(s)
      島田敦広、馬場淳平、山下栄樹、村本和優、伊藤-新澤恭子、月原冨武、吉川信也
    • Organizer
      第16回日本蛋白質科学会年会
    • Place of Presentation
      福岡国際会議場(福岡市)
    • Year and Date
      2016-06-07
    • Related Report
      2016 Research-status Report
  • [Presentation] 呼吸鎖電子伝達系プロトンポンプ性酸素還元酵素の電子伝達活性2015

    • Author(s)
      村本和優
    • Organizer
      日本生体エネルギー研究会第41回討論会
    • Place of Presentation
      東京大学 文京区
    • Year and Date
      2015-12-21
    • Related Report
      2015 Research-status Report
  • [Presentation] Co-crystallization and kinetic analysis of nitric oxide reductase and electron transfer proteins from Pseudomonas aeruginosa.2015

    • Author(s)
      Oobatake K, Muramoto K, Tosha T, Shiro Y
    • Organizer
      第3回ピコバイオロジー研究所国際シンポジウム
    • Place of Presentation
      兵庫県立先端科学技術支援センター 赤穂郡上郡町
    • Year and Date
      2015-12-08
    • Related Report
      2015 Research-status Report
    • Int'l Joint Research
  • [Presentation] Purification and crystallization of quinol-dependent nitric oxide reductase from Neisseria meningitides.2015

    • Author(s)
      Yuki R, Muramoto K, Tosha T, Shiro Y
    • Organizer
      第3回ピコバイオロジー研究所国際シンポジウム
    • Place of Presentation
      兵庫県立先端科学技術支援センター 赤穂郡上郡町
    • Year and Date
      2015-12-08
    • Related Report
      2015 Research-status Report
    • Int'l Joint Research
  • [Presentation] Structural analysis of respiratory O2 reductase in the reaction intermediate state.2015

    • Author(s)
      村本和優
    • Organizer
      日本生物物理学会第53回年会
    • Place of Presentation
      金沢大学 金沢市
    • Year and Date
      2015-09-13
    • Related Report
      2015 Research-status Report
  • [Presentation] 緑膿菌由来一酸化窒素還元酵素と電子供与タンパク質の精製及び共結晶化2015

    • Author(s)
      大畠海人、村本和優、當舎武彦、城宜嗣
    • Organizer
      第15回日本蛋白質科学会年会
    • Place of Presentation
      あわぎんホール 徳島市
    • Year and Date
      2015-06-24
    • Related Report
      2015 Research-status Report
  • [Presentation] 高分解能X 線結晶構造解析によるチトクロム酸化酵素の酸素還元・プロトンポンプ機構の解明2015

    • Author(s)
      月原冨武、島田敦広、島田悟、武村秀平、馬場淳平、村本和優、山下栄樹、伊藤・新澤恭子、吉川信也
    • Organizer
      第15回日本蛋白質科学会年会
    • Place of Presentation
      あわぎんホール 徳島市
    • Year and Date
      2015-06-24
    • Related Report
      2015 Research-status Report
  • [Presentation] チトクロム酸化酵素内のMgを含む水クラスターが酸素取り込み前に4等量のプロトンを蓄積することで、高効率プロトンポンプ反応を可能にする2015

    • Author(s)
      島田敦広、矢野直峰、武村秀平、村本和優、山下栄樹、伊藤・新澤恭子、前田友子、月原冨武、吉川信也
    • Organizer
      第15回日本蛋白質科学会年会
    • Place of Presentation
      あわぎんホール 徳島市
    • Year and Date
      2015-06-24
    • Related Report
      2015 Research-status Report
  • [Presentation] Purification and co-crystallization of nitric oxide reductase and electron transfer proteins from Pseudomonas aeruginosa.2015

    • Author(s)
      Oobatake K, Muramoto K, Tosya T, Shiro Y
    • Organizer
      RIKEN Symposium “Metals in Biology” in Wako
    • Place of Presentation
      理研和光キャンパス 和光市
    • Year and Date
      2015-06-16
    • Related Report
      2015 Research-status Report

URL: 

Published: 2015-04-16   Modified: 2019-03-29  

Information User Guide FAQ News Terms of Use Attribution of KAKENHI

Powered by NII kakenhi