The torque generated by bacterial flagellar motors measured using single-molecule detection techniques
| Project/Area Number |
15K07034
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| Research Category |
Grant-in-Aid for Scientific Research (C)
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| Allocation Type | Multi-year Fund |
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| Section | 一般 |
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| Research Field |
Biophysics
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| Research Institution | Hosei University |
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Principal Investigator |
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| Research Collaborator |
KAWAGISHI Ikuro 法政大学, 生命科学部, 教授 (80234037)
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| Project Period (FY) |
2015-04-01 – 2018-03-31
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| Project Status |
Completed (Fiscal Year 2017)
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| Budget Amount *help |
¥4,940,000 (Direct Cost: ¥3,800,000、Indirect Cost: ¥1,140,000)
Fiscal Year 2017: ¥1,430,000 (Direct Cost: ¥1,100,000、Indirect Cost: ¥330,000)
Fiscal Year 2016: ¥1,430,000 (Direct Cost: ¥1,100,000、Indirect Cost: ¥330,000)
Fiscal Year 2015: ¥2,080,000 (Direct Cost: ¥1,600,000、Indirect Cost: ¥480,000)
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| Keywords | 細菌べん毛 / 1分子計測 / 細菌べん毛モーター / 光ピンセット / ナノ計測 |
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| Outline of Final Research Achievements |
The bacterial flagellar motor is a rotary nano-machine. The maximum torque of Escherichia coli motor was measured in the range from ~1300 to ~4500 pNnm, depending on the reports. In this research, we directly measured the generated torque by an optical trap. Rotation of an E. coli cell tethered to coverslip via filament was stopped by a trapped 1 um bead. By measuring the displacement of the bead, we determined that the motor generates ~4500 pNnm. However, we also found that the optical trap effects on not only a bead but also a cell body itself. Then, by removing the side-effect of an optical trap, the generated toque of the motor was determined to be ~1800 pNnm.
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Report
(4 results)
Research Products
(27 results)
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[Journal Article] Substrate-dependent dynamics of the multidrug efflux transporter AcrB of Escherichia coli2016
Author(s)
Yamamoto, K., Tamai, R., Yamazaki, M., Inaba, T., Sowa, Y. & Kawagishi, I
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Journal Title
Sci Rep
Volume: 6
Issue: 1
Pages: 21909-21909
DOI
Related Report
Peer Reviewed / Open Access
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