Analysis of reletionship between RNF213 moyamoya factor and autophagy
Project/Area Number |
15K07044
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Research Category |
Grant-in-Aid for Scientific Research (C)
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Allocation Type | Multi-year Fund |
Section | 一般 |
Research Field |
Cell biology
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Research Institution | Mukogawa Women's University |
Principal Investigator |
Habu Toshiyuki 武庫川女子大学, 生活環境学部, 准教授 (70346071)
|
Co-Investigator(Renkei-kenkyūsha) |
KOIZUMI Akio 京都大学, 医学研究科, 教授 (50124574)
KOBAYASHI Hatasu 中部大学, 生命健康科学部, 准教授 (70542091)
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Project Period (FY) |
2015-04-01 – 2018-03-31
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Project Status |
Completed (Fiscal Year 2017)
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Budget Amount *help |
¥5,070,000 (Direct Cost: ¥3,900,000、Indirect Cost: ¥1,170,000)
Fiscal Year 2017: ¥650,000 (Direct Cost: ¥500,000、Indirect Cost: ¥150,000)
Fiscal Year 2016: ¥1,690,000 (Direct Cost: ¥1,300,000、Indirect Cost: ¥390,000)
Fiscal Year 2015: ¥2,730,000 (Direct Cost: ¥2,100,000、Indirect Cost: ¥630,000)
|
Keywords | モヤモヤ病 / オートファジー / 脂質代謝 / 酸素環境 / 低酸素環境 / 脂肪滴 / 血管内皮細胞 |
Outline of Final Research Achievements |
P.R4810K of RNF213 (mysterin: rs112735431), which is an AAA(+) ATPase, is the susceptibility polymorphism for moyamoya disease (MMD) in East Asians. However, the role of RNF213 R4810K in the etiology of MMD is unknown.To clarify the role of RNF213, RNF213 overexpressed in human culture cells was subjected to microscopic analysis. Using various mutated RNF213 overexpressing cells, morphological cahanges of autopagosomes were found and mobility of cells movement were largely different between wt of RNF213 and SNPs, mutants .RNF213 protein function in moyamoya disease was still unknown but from this analysis we concluded SNPs of this disease might affect the interaction of intermolecule RNF213 domain.
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Report
(4 results)
Research Products
(9 results)
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[Journal Article] PTP1B controls non-mitochondrial oxygen consumption by regulating RNF213 to promote tumour survival during hypoxia.2016
Author(s)
Banh RS, Iorio C, Marcotte R, Xu Y, Cojocari D, Rahman AA, Pawling J, Zhang W, Sinha A, Rose CM, Isasa M, Zhang S, Wu R, Virtanen C, Hitomi T, Habu T, Sidhu SS, Koizumi A, Wilkins SE, Kislinger T, Gygi SP, Schofield CJ, Dennis JW, Wouters BG, Neel BG.
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Journal Title
Nat Cell Biol
Volume: 18
Issue: 7
Pages: 803-813
DOI
Related Report
Peer Reviewed / Int'l Joint Research
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[Journal Article] RNF213 rare variants in Slovakian and Czech moyamoya disease patients2016
Author(s)
Hatasu Kobayashi, Miroslav Brozman, Kateina Kyselova, DaaViszlayova, Takaaki Morimoto, Martin Roubec, David koloudik, Andrea Petroviova , Dominik Juskani,Jozef Strauss , Marian Halaj, Peter Kurray , Marian Hranai, Koji Harada, Sumiko Inoue ,Toshiyuki Habu, Roman Herzig , Akio Koizumi
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Journal Title
PLOS ONE
Volume: 11
Issue: 10
Pages: e0164759-e0164759
DOI
NAID
Related Report
Peer Reviewed / Open Access / Int'l Joint Research
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[Journal Article] Biochemical and Functional Characterization of RNF213 (Mysterin) R4810K, a Susceptibility Mutation of Moyamoya Disease, in Angiogenesis In Vitro and In Vivo.2015
Author(s)
Kobayashi H, Matsuda Y, Hitomi T, Okuda H, Shioi H, Matsuda T, Imai H, Sone M, Taura D, Harada KH, Habu T, Takagi Y, Miyamoto S, Koizumi A.
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Journal Title
J Am Heart Assoc.
Volume: 30;4(7)
Issue: 7
DOI
Related Report
Peer Reviewed / Open Access
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[Presentation] In vitro and in vivo evidence on inhibition of angiogenesis by the Moyamoya susceptible gene and its mutation, RNF213 R4810K2015
Author(s)
Akio Koizumi, Hatasu Kobayashi, Yoshiko Matsuda, Toshiaki Hitomi, Hiroko Okuda, Hirotomo Shioi, Tetsuya Matsuda, Hirohiko Imai, Masakatsu Sone, Daisuke Taura, Kouji H. Harada, Yasushi Takagi, Susumu Miyamoto, Toshiyuki Habu
Organizer
4th International Moyamoya Meeting Berlin
Place of Presentation
Kaiserin-Friedrich-Haus, Germany
Year and Date
2015-07-02
Related Report
Int'l Joint Research
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[Presentation] PTP1B regulates the Moyamoya disease-associated E3 ligase, RNF213 and cellular dioxygenase activity to allow breast tumor survival in hypoxia2015
Author(s)
Robert S. Banh, Caterina Iorio, Richard Marcotte1, Yang Xu, Dan Cojocari, Anas Abdel Rahman, Judy Pawling, Ankit Sinha, Toshiaki Hitomi, Toshiyuki Habu, Akio Koizumi, Sarah Wilkins, Thomas Kislinger, Christopher J. Schofield, James W. Dennis, Bradly G. Wouters, and Benjamin G. Neel
Organizer
AACR 106th Annual Meeting 2015
Place of Presentation
Philadelphia Convention Center, USA
Year and Date
2015-04-18
Related Report
Int'l Joint Research