| Budget Amount *help |
¥5,200,000 (Direct Cost: ¥4,000,000、Indirect Cost: ¥1,200,000)
Fiscal Year 2017: ¥1,560,000 (Direct Cost: ¥1,200,000、Indirect Cost: ¥360,000)
Fiscal Year 2016: ¥1,560,000 (Direct Cost: ¥1,200,000、Indirect Cost: ¥360,000)
Fiscal Year 2015: ¥2,080,000 (Direct Cost: ¥1,600,000、Indirect Cost: ¥480,000)
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| Outline of Final Research Achievements |
Smooth septate junctions (sSJs) are cell-cell junctions that regulate the paracellular pathway of the endodermally-derived epithelial cells in arthropods. Previously, we identified Ssk and Mesh as specific components of sSJs in Drosophila. To understand the molecular mechanisms involved in sSJ formation, we carried out a genetic screen of a Drosophila chromosomal deficiency stock and identified several strains defective in the localization of Mesh at sSJs. By analyzing these strains, we found that the genes encoding tetraspanin, Tsp2A and a novel single membrane-spanning protein, CG13704 were responsible for the phenotype. Both proteins specifically localized at sSJs and were required for sSJ formation as well as the barrier function of the midgut. Furthermore, we found that Tsp2A, CG13704, Ssk and Mesh form a complex and were interdependent for their sSJ localization. Therefore, we concluded that these sSJ-proteins act together to organize sSJs.
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