| Project/Area Number |
15K07051
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| Research Category |
Grant-in-Aid for Scientific Research (C)
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| Allocation Type | Multi-year Fund |
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| Section | 一般 |
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| Research Field |
Cell biology
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| Research Institution | Hiroshima University |
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Principal Investigator |
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| Co-Investigator(Kenkyū-buntansha) |
仙谷 和弘 広島大学, 医歯薬保健学研究科(医), 講師 (30508164)
横崎 恭之 広島大学, 保健管理センター, 准教授 (80210607)
外丸 祐介 広島大学, 自然科学研究支援開発センター, 教授 (90309352)
佐久間 哲史 広島大学, 理学研究科, 特任講師 (90711143)
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| Project Period (FY) |
2015-04-01 – 2018-03-31
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| Project Status |
Completed (Fiscal Year 2017)
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| Budget Amount *help |
¥5,200,000 (Direct Cost: ¥4,000,000、Indirect Cost: ¥1,200,000)
Fiscal Year 2017: ¥1,950,000 (Direct Cost: ¥1,500,000、Indirect Cost: ¥450,000)
Fiscal Year 2016: ¥1,430,000 (Direct Cost: ¥1,100,000、Indirect Cost: ¥330,000)
Fiscal Year 2015: ¥1,820,000 (Direct Cost: ¥1,400,000、Indirect Cost: ¥420,000)
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| Keywords | オステオポンチン / 線維化 / 翻訳後修飾 / ゲノム編集 / 抗体 / 炎症 |
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| Outline of Final Research Achievements |
In this study, we examined to reveal the role of post-translational modification such as thrombin-cleaved and transglutaminase-polymerized osteopontin on fibrosis. As a result, at first, we succeeded generation of mice expressing thrombin-cleaved formed or transglutaminase-polymerized formed of osteopontin by genome editing. But the importance of these post-translational modification of osteopontin on fibrosis was not cleared in this study. We were successful to construct polymeric human osteopontin specific ELISA system.
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