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Structural and functional analysis of cell networks within taste buds.

Research Project

Project/Area Number 15K07053
Research Category

Grant-in-Aid for Scientific Research (C)

Allocation TypeMulti-year Fund
Section一般
Research Field Cell biology
Research InstitutionKyushu Institute of Technology

Principal Investigator

Ohtubo Yoshitaka  九州工業大学, 大学院生命体工学研究科, 准教授 (00380725)

Project Period (FY) 2015-04-01 – 2018-03-31
Project Status Completed (Fiscal Year 2017)
Budget Amount *help
¥4,940,000 (Direct Cost: ¥3,800,000、Indirect Cost: ¥1,140,000)
Fiscal Year 2017: ¥1,040,000 (Direct Cost: ¥800,000、Indirect Cost: ¥240,000)
Fiscal Year 2016: ¥1,560,000 (Direct Cost: ¥1,200,000、Indirect Cost: ¥360,000)
Fiscal Year 2015: ¥2,340,000 (Direct Cost: ¥1,800,000、Indirect Cost: ¥540,000)
Keywords傍分泌 / ヘミチャネル / アセチルコリン受容体 / ATP受容体 / 電位依存性Kチャネル / パッチクランプ法 / 細胞間情報伝達 / 味神経線維 / 電位依存性Kチャネル / 遺伝子サブタイプ / 細胞型 / 神経線維 / 味蕾構造 / パッチクランプ
Outline of Final Research Achievements

Taste buds consist of the heterogeneous population of taste bud cells with different cell types that may make cell-to-cell communications within taste buds. I show that the electrophysiological properties of ion channels for paracrine signaling, the cell-type dependent expression of the channel and neurotransmitter receptors, and the ratio of cell types expressing the channel and receptors in a single taste bud. The discussion is made on the role of these channels and receptors in the transmission of taste responses.

Report

(4 results)
  • 2017 Annual Research Report   Final Research Report ( PDF )
  • 2016 Research-status Report
  • 2015 Research-status Report
  • Research Products

    (9 results)

All 2017 2016 2015 Other

All Journal Article (1 results) (of which Peer Reviewed: 1 results,  Open Access: 1 results,  Acknowledgement Compliant: 1 results) Presentation (7 results) (of which Int'l Joint Research: 2 results) Remarks (1 results)

  • [Journal Article] Selective expression of muscarinic acetylcholine receptor subtype M3 by mouse type III taste bud cells.2016

    • Author(s)
      Mori Y, Eguchi K, Yoshii K, Ohtubo Y
    • Journal Title

      Pflugers Archiv : European journal of physiology.

      Volume: 468(11) Issue: 11-12 Pages: 2053-2059

    • DOI

      10.1007/s00424-016-1879-5

    • Related Report
      2016 Research-status Report
    • Peer Reviewed / Open Access / Acknowledgement Compliant
  • [Presentation] Voltage-gated channels and neurotransmitter receptors in mouse taste bud cells.2017

    • Author(s)
      Ohtubo Y
    • Organizer
      The 51th Annual Meeting of the Japanese Association for the Study of Taste and Smell
    • Related Report
      2017 Annual Research Report
  • [Presentation] Electrophysiological properties of A-type potassium currents in mouse fungiform taste bud cells2017

    • Author(s)
      Moribayashi T, Ohtubo Y
    • Organizer
      The 51th Annual Meeting of the Japanese Association for the Study of Taste and Smell
    • Related Report
      2017 Annual Research Report
  • [Presentation] Electrophysiological study on ATP-permeable voltage-gated ion channels in type II cells of mouse taste bud cells.2016

    • Author(s)
      Ohtubo Y, Takashima M
    • Organizer
      10th Federation of European Neuroscience Societies (FENS)
    • Place of Presentation
      Copenhagen, Denmark
    • Year and Date
      2016-07-02
    • Related Report
      2016 Research-status Report
    • Int'l Joint Research
  • [Presentation] Numerical studies on the expression of cell-type marker proteins in mouse foliate and circumvallate taste buds.2016

    • Author(s)
      Ogata T, Ohtubo Y
    • Organizer
      17th International Symposium on Olfaction and Taste (ISOT2016)
    • Place of Presentation
      Yokohama, Japan
    • Year and Date
      2016-06-05
    • Related Report
      2016 Research-status Report
    • Int'l Joint Research
  • [Presentation] 葉状乳頭味蕾における細胞型マーカー分子発現細胞の定量的解析2015

    • Author(s)
      緒方貴宏、大坪義孝
    • Organizer
      日本味と匂学会第49回大会
    • Place of Presentation
      じゅうろくプラザ、岐阜市
    • Year and Date
      2015-09-24
    • Related Report
      2015 Research-status Report
  • [Presentation] マウス茸状乳頭味蕾細胞のCs 非感受性外向き電流に対する各種ブロッカーの効果2015

    • Author(s)
      高島麻都花、大坪義孝
    • Organizer
      日本味と匂学会第49回大会
    • Place of Presentation
      じゅうろくプラザ、岐阜市
    • Year and Date
      2015-09-24
    • Related Report
      2015 Research-status Report
  • [Presentation] 単一味蕾に発現する biocytin 取り込み細胞数の定量的薬理学的解析2015

    • Author(s)
      岩本正史、大坪義孝
    • Organizer
      日本味と匂学会第49回大会
    • Place of Presentation
      じゅうろくプラザ、岐阜市
    • Year and Date
      2015-09-24
    • Related Report
      2015 Research-status Report
  • [Remarks] 大坪研究室のホームページ

    • URL

      http://www.brain.kyutech.ac.jp/~otsubo/index.html

    • Related Report
      2016 Research-status Report

URL: 

Published: 2015-04-16   Modified: 2019-03-29  

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