| Budget Amount *help |
¥4,940,000 (Direct Cost: ¥3,800,000、Indirect Cost: ¥1,140,000)
Fiscal Year 2018: ¥1,170,000 (Direct Cost: ¥900,000、Indirect Cost: ¥270,000)
Fiscal Year 2017: ¥1,170,000 (Direct Cost: ¥900,000、Indirect Cost: ¥270,000)
Fiscal Year 2016: ¥1,170,000 (Direct Cost: ¥900,000、Indirect Cost: ¥270,000)
Fiscal Year 2015: ¥1,430,000 (Direct Cost: ¥1,100,000、Indirect Cost: ¥330,000)
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| Outline of Final Research Achievements |
Ras is one of the small GTPase that regulates key cell functions such as cell proliferation and differentiation, gene expression, and cell adhesion. In addition, mutations at the amino acids involved in GTP hydrolysis has been shown to induce canceration of cells. Since the structure of Ras and its molecular mechanism as a biomolecular machine have been well studied, it will be possible to control of cell function if Ras can be regulated by an artificial external stimulus utilizing the ingenious mechanism of Ras. In this study, we attempted to control the function of Ras reversibly by introducing photo responsive photochromic molecules as a light switch into the mechanical mechanism of Ras and the regulating factor of Ras. And we clarified that the GTPase activity of Ras is controlled reversibly. In addition, it was shown that this photochromic peptide can be used to control the GDP-GTP exchange reaction of Ras in a photoreversible manner.
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