Characterization of novel mechanisms that maintain bacterial cell division under alkaline condition.

Research Project

Project/Area Number	15K07061
Research Category	Grant-in-Aid for Scientific Research (C)
Allocation Type	Multi-year Fund
Section	一般
Research Field	Cell biology
Research Institution	Nigata University of Phermacy and Applied Life Sciences
Principal Investigator	Yamaguchi Toshio 新潟薬科大学, 薬学部, 准教授 (50434452)
Project Period (FY)	2015-04-01 – 2018-03-31
Project Status	Completed (Fiscal Year 2017)
Budget Amount *help	¥4,940,000 (Direct Cost: ¥3,800,000、Indirect Cost: ¥1,140,000) Fiscal Year 2017: ¥910,000 (Direct Cost: ¥700,000、Indirect Cost: ¥210,000) Fiscal Year 2016: ¥910,000 (Direct Cost: ¥700,000、Indirect Cost: ¥210,000) Fiscal Year 2015: ¥3,120,000 (Direct Cost: ¥2,400,000、Indirect Cost: ¥720,000)
Keywords	DedD / 細胞分裂 / 大腸菌 / アルカリ耐性 / divisome / 細菌 / ストレス耐性
Outline of Final Research Achievements	During the course of the genome-wide screening, we found that E. coli dedD gene is essential for the maintenance of cell division under alkaline growth condition. The cell division deficiency of the dedD deletion strain was not dependent of the presence of SOS and NO pathway, and the localization of FtsN in this strain was perturbed under alkaline condition. The BACTH assay indicated potential interactions between DedD and several other divisome proteins. These results suggested that DedD might act as a scaffold that would keep maintaining proper assembly of divisome components under alkaline condition.

Report

(4 results)