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Analysis of molecular mechanismthat regulate extracellular localization of basement membranes .

Research Project

Project/Area Number 15K07063
Research Category

Grant-in-Aid for Scientific Research (C)

Allocation TypeMulti-year Fund
Section一般
Research Field Cell biology
Research InstitutionAriake National College of Technology (2017)
National Institute of Genetics (2015-2016)

Principal Investigator

Ihara shinji  有明工業高等専門学校, 創造工学科, 准教授 (70373272)

Project Period (FY) 2015-04-01 – 2018-03-31
Project Status Completed (Fiscal Year 2017)
Budget Amount *help
¥5,070,000 (Direct Cost: ¥3,900,000、Indirect Cost: ¥1,170,000)
Fiscal Year 2017: ¥1,170,000 (Direct Cost: ¥900,000、Indirect Cost: ¥270,000)
Fiscal Year 2016: ¥1,820,000 (Direct Cost: ¥1,400,000、Indirect Cost: ¥420,000)
Fiscal Year 2015: ¥2,080,000 (Direct Cost: ¥1,600,000、Indirect Cost: ¥480,000)
Keywords基底膜 / 小胞体 / GPI / フォールディング / 線虫 / GPIアンカー
Outline of Final Research Achievements

The basement membranes (BM) are dense sheets structure of protein complex composed by secreted proteins, providing structural support, barrier function, and maintenance of cell polarity. Caenorhabditis elegans (C. elegans) is a semi-transparent soil nematode that is useful for fluorescence tag to visualize proteins. To know different localization patterning of BM structural proteins in vivo, we visualized type IV Collagen that is major basement membrane protein. Using these transgenic lines, we have initiated ethyl methanesulfonate mutagenesis screening to isolate mutants showing aberrant accumulation or distribution of BM components. Cloning of the relevant gene revealed that it encodes pigN that is generally required for glycosylphosphatidylinositol (GPI) maturation. Interestingly, protein accumulations have not observed in the mutants of other PIG genes, indicating that PIGN have unique function for proteins secretion.

Report

(4 results)
  • 2017 Annual Research Report   Final Research Report ( PDF )
  • 2016 Research-status Report
  • 2015 Research-status Report
  • Research Products

    (5 results)

All 2017 2016 2015 Other

All Journal Article (2 results) (of which Int'l Joint Research: 1 results,  Peer Reviewed: 2 results,  Open Access: 1 results) Presentation (2 results) Remarks (1 results)

  • [Journal Article] PIGN prevents protein aggregation in the endoplasmic reticulum independently of its function in the GPI synthesis.2017

    • Author(s)
      Ihara S, Nakayama S, Murakami Y, Suzuki E, Asakawa M, Kinoshita T, Sawa H.
    • Journal Title

      Journal of Cell Science

      Volume: 130 Issue: 3 Pages: 602-613

    • DOI

      10.1242/jcs.196717

    • Related Report
      2017 Annual Research Report 2016 Research-status Report
    • Peer Reviewed
  • [Journal Article] SPARC Promotes Cell Invasion In Vivo by Decreasing Type IV Collagen Levels in the Basement Membrane2016

    • Author(s)
      Meghan A. Morrissey, Ranjay Jayadev, Ginger R. Miley, Catherine A. Blebea, Qiuyi Chi, Shinji Ihara, David R. Sherwood
    • Journal Title

      PLOS Genetics

      Volume: 12 Issue: 2 Pages: 1-24

    • DOI

      10.1371/journal.pgen.1005905

    • Related Report
      2015 Research-status Report
    • Peer Reviewed / Open Access / Int'l Joint Research
  • [Presentation] GPIアンカー修飾酵素PIGNの非古典的機能の解析2017

    • Author(s)
      伊原 伸治
    • Organizer
      比較グライコーム研究会
    • Related Report
      2017 Annual Research Report
  • [Presentation] PIGNは小胞体のATP量を維持する事で、タンパク質の分泌を制御している2015

    • Author(s)
      伊原伸治
    • Organizer
      第38回 日本分子生物学会
    • Place of Presentation
      神戸ポートアイランド
    • Year and Date
      2015-12-01
    • Related Report
      2015 Research-status Report
  • [Remarks] 小胞体内でタンパク質凝集を抑えるPIGNの新しい機能の発見

    • URL

      https://www.nig.ac.jp/nig/ja/2017/03/research-highlights_ja/20170314-2.html

    • Related Report
      2017 Annual Research Report

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Published: 2015-04-16   Modified: 2019-03-29  

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