| Budget Amount *help |
¥5,070,000 (Direct Cost: ¥3,900,000、Indirect Cost: ¥1,170,000)
Fiscal Year 2017: ¥1,170,000 (Direct Cost: ¥900,000、Indirect Cost: ¥270,000)
Fiscal Year 2016: ¥1,820,000 (Direct Cost: ¥1,400,000、Indirect Cost: ¥420,000)
Fiscal Year 2015: ¥2,080,000 (Direct Cost: ¥1,600,000、Indirect Cost: ¥480,000)
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| Outline of Final Research Achievements |
The basement membranes (BM) are dense sheets structure of protein complex composed by secreted proteins, providing structural support, barrier function, and maintenance of cell polarity. Caenorhabditis elegans (C. elegans) is a semi-transparent soil nematode that is useful for fluorescence tag to visualize proteins. To know different localization patterning of BM structural proteins in vivo, we visualized type IV Collagen that is major basement membrane protein. Using these transgenic lines, we have initiated ethyl methanesulfonate mutagenesis screening to isolate mutants showing aberrant accumulation or distribution of BM components. Cloning of the relevant gene revealed that it encodes pigN that is generally required for glycosylphosphatidylinositol (GPI) maturation. Interestingly, protein accumulations have not observed in the mutants of other PIG genes, indicating that PIGN have unique function for proteins secretion.
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