| Budget Amount *help |
¥5,200,000 (Direct Cost: ¥4,000,000、Indirect Cost: ¥1,200,000)
Fiscal Year 2017: ¥910,000 (Direct Cost: ¥700,000、Indirect Cost: ¥210,000)
Fiscal Year 2016: ¥2,340,000 (Direct Cost: ¥1,800,000、Indirect Cost: ¥540,000)
Fiscal Year 2015: ¥1,950,000 (Direct Cost: ¥1,500,000、Indirect Cost: ¥450,000)
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| Outline of Final Research Achievements |
The importinβ family proteins are nucleocytoplasmic transport receptors that mediate the migration of most nuclear proteins. In this study, we identified the nuclear import cargo proteins of the 12 species of human importins by a high-throughput method called SILAC-Tp that utilizes stable isotope labeling of cells, an in vitro nuclear transport system and mass spectrometry. The results elucidated the cellular processes linked to each importin through the cargoes. The results also suggested an unexpected variety of the modes of importin-cargo interaction, and we analyzed the variation of the cargo-binding sites on two importins by mutagenesis and in vitro binding assays.
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