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Elucidation of gene expression regulation mechanism in cancer cells focusing on multifunctionality of Importin-alpha

Research Project

Project/Area Number 15K07068
Research Category

Grant-in-Aid for Scientific Research (C)

Allocation TypeMulti-year Fund
Section一般
Research Field Cell biology
Research InstitutionNational Institutes of Biomedical Innovation, Health and Nutrition

Principal Investigator

MIYAMOTO Yoichi  国立研究開発法人医薬基盤・健康・栄養研究所, 医薬基盤研究所 細胞核輸送ダイナミクスプロジェクト, サブプロジェクトリーダー (10379084)

Co-Investigator(Renkei-kenkyūsha) OKA Masahiro  国立研究開発法人医薬基盤・健康・栄養研究所, 医薬基盤研究所 細胞核輸送ダイナミクスプロジェクト, プロジェクトリーダー (40432504)
YONEDA Yoshihiro  国立研究開発法人医薬基盤・健康・栄養研究所, 医薬基盤研究所, 研究所長 (80191667)
FUJII Satoshi  国立研究開発法人国立がん研究センター, 先端医療開発センター, ユニット長 (30314743)
TOMONAGA Takeshi  国立研究開発法人医薬基盤・健康・栄養研究所, 医薬基盤研究所・プロテオームリサーチプロジェクト, プロジェクトリーダー (80227644)
SHIROMIZU Takashi  三重大学, 医学(系)研究科(研究院), 助教 (00582678)
Project Period (FY) 2015-04-01 – 2018-03-31
Project Status Completed (Fiscal Year 2017)
Budget Amount *help
¥5,070,000 (Direct Cost: ¥3,900,000、Indirect Cost: ¥1,170,000)
Fiscal Year 2017: ¥1,300,000 (Direct Cost: ¥1,000,000、Indirect Cost: ¥300,000)
Fiscal Year 2016: ¥1,430,000 (Direct Cost: ¥1,100,000、Indirect Cost: ¥330,000)
Fiscal Year 2015: ¥2,340,000 (Direct Cost: ¥1,800,000、Indirect Cost: ¥540,000)
Keywords核輸送 / インポーティン / がん / 転写調節 / 核局在化シグナル / クロマチン / Importin alpha / 遺伝子発現制御 / NLS / Importin-α / 乳がん / 細胞亜型
Outline of Final Research Achievements

The aim of this study is to understand how Importin-α1, the nuclear-cytoplasmic transport factor, contributes specific gene expression in cancer cells. Our results reveal that Importin-α1 highly expresses in several breast cancer cell lines, and specifically localizes in the nucleus. Using the chromatin immunoprecipitation sequence and microarray analysis, we demonstrate that Importin-α1 interacts with chromatins and involves in gene regulation of cancer cells in addition to the nuclear transport function.

Report

(4 results)
  • 2017 Annual Research Report   Final Research Report ( PDF )
  • 2016 Research-status Report
  • 2015 Research-status Report
  • Research Products

    (14 results)

All 2017 2016 2015

All Journal Article (8 results) (of which Int'l Joint Research: 2 results,  Peer Reviewed: 7 results,  Open Access: 6 results,  Acknowledgement Compliant: 3 results) Presentation (4 results) (of which Int'l Joint Research: 2 results,  Invited: 1 results) Book (1 results) Patent(Industrial Property Rights) (1 results)

  • [Journal Article] Development of a pipeline for automated, high-throughput analysis of paraspeckle proteins reveals specific roles for importin α proteins.2017

    • Author(s)
      Major A.T., Miyamoto Y., Lo C., Jans D.A., Loveland K.L.
    • Journal Title

      Sci. Rep.

      Volume: 7 Issue: 1 Pages: 43323-43323

    • DOI

      10.1038/srep43323

    • Related Report
      2016 Research-status Report
    • Peer Reviewed / Open Access / Int'l Joint Research
  • [Journal Article] Molecular Mechanism of HIV-1 Vpr for Binding to Importin-α.2016

    • Author(s)
      Miyatake H., Sanjoh A., Murakami T., Murakami H., Matsuda G., Hagiwara K., Yokoyama M., Sato H., Miyamoto Y., Dohmae N., Aida Y.
    • Journal Title

      J. Mol. Biol.

      Volume: 428 Issue: 13 Pages: 2744-2757

    • DOI

      10.1016/j.jmb.2016.05.003

    • Related Report
      2016 Research-status Report
    • Peer Reviewed
  • [Journal Article] Importin α: a key molecule in nuclear transport and non-transport functions.2016

    • Author(s)
      Miyamoto Y, Yamada K, Yoneda Y.
    • Journal Title

      J Biochem

      Volume: 160 Issue: 2 Pages: 69-75

    • DOI

      10.1093/jb/mvw036

    • NAID

      40020914020

    • Related Report
      2016 Research-status Report
    • Peer Reviewed / Open Access / Int'l Joint Research / Acknowledgement Compliant
  • [Journal Article] Data on dimer formation between importin α subtypes2016

    • Author(s)
      Miyamoto Y., Oka M.
    • Journal Title

      Data in Brief

      Volume: 7 Pages: 1248-1253

    • DOI

      10.1016/j.dib.2016.03.080

    • Related Report
      2015 Research-status Report
    • Peer Reviewed / Open Access / Acknowledgement Compliant
  • [Journal Article] Cell surface localization of importin α1/KPNA2 affects cancer cell proliferation by regulating FGF1 signalling2016

    • Author(s)
      Yamada K., Miyamoto Y., Tsujii A., Moriyama T., Ikuno Y., Shiromizu T., Serada S., Fujimoto M., Tomonaga T., Naka T., Yoneda Y., Oka M.
    • Journal Title

      Scientific Reports

      Volume: 6 Issue: 1 Pages: 231410-231410

    • DOI

      10.1038/srep21410

    • Related Report
      2015 Research-status Report
    • Peer Reviewed / Open Access
  • [Journal Article] Retinoblastoma Binding Protein 4-Regulated Classical Nuclear Transport Is Involved in Cellular Senescence2015

    • Author(s)
      Tsujii A., Miyamoto Y., Moriyama T., Tsuchiya T., Obuse C., Mizuguchi K., Oka M., Yoneda Y.
    • Journal Title

      The Journal of Biological Chemistry

      Volume: 290 Issue: 49 Pages: 29375-29388

    • DOI

      10.1074/jbc.m115.681908

    • Related Report
      2015 Research-status Report
    • Peer Reviewed / Open Access
  • [Journal Article] Functional characterization of importin α8 as a classical nuclear localization signal receptor2015

    • Author(s)
      Kimoto C., Moriyama T., Tsujii A., IgarashiY., Obuse C., Miyamoto Y., Oka M., Yoneda Y.
    • Journal Title

      Biochimica et Biophysica Acta (BBA) - Molecular Cell Research

      Volume: 1853 Issue: 10 Pages: 2676-2683

    • DOI

      10.1016/j.bbamcr.2015.07.017

    • Related Report
      2015 Research-status Report
    • Peer Reviewed / Open Access / Acknowledgement Compliant
  • [Journal Article] 創薬ターゲットとしての核-細胞質間物質輸送2015

    • Author(s)
      宮本洋一
    • Journal Title

      バイオサイエンス&インダストリー

      Volume: 73 Pages: 288-291

    • NAID

      40020536325

    • Related Report
      2015 Research-status Report
  • [Presentation] 生殖細胞形成とストレス応答におけるSTK35L1の役割2017

    • Author(s)
      宮本洋一、Penny A.F. Whiley、岡正啓、Kate L. Loveland
    • Organizer
      日本細胞生物学会
    • Related Report
      2017 Annual Research Report
  • [Presentation] Regulation mechanism of a classical nuclear protein transport through importin α2015

    • Author(s)
      Yoichi Miyamoto
    • Organizer
      International Symposium on Biomolecular Sciences
    • Place of Presentation
      宮崎
    • Year and Date
      2015-12-17
    • Related Report
      2015 Research-status Report
    • Int'l Joint Research / Invited
  • [Presentation] 新規核局在化シグナル受容体importin α8の機能解析2015

    • Author(s)
      宮本洋一、盛山哲嗣、木本千裕、辻井聡、五十嵐芳暢、小布施力史、岡正啓、米田悦啓
    • Organizer
      BMB2015
    • Place of Presentation
      神戸
    • Year and Date
      2015-12-01
    • Related Report
      2015 Research-status Report
  • [Presentation] A novel regulator of a classical nuclear transport pathway and cellular senescence2015

    • Author(s)
      Yoichi Miyamoto
    • Organizer
      Nuclear Transport Meeting
    • Place of Presentation
      スペイン
    • Year and Date
      2015-09-18
    • Related Report
      2015 Research-status Report
    • Int'l Joint Research
  • [Book] Nuclear Architecture and Dynamics2017

    • Author(s)
      Miyamoto Y., Yoneda Y., Oka M.
    • Total Pages
      569
    • Publisher
      Academic press
    • ISBN
      9780128034804
    • Related Report
      2017 Annual Research Report
  • [Patent(Industrial Property Rights)] 精神疾患モデル動物およびその製造方法2017

    • Inventor(s)
      岡正啓、盛山哲嗣、米田悦啓、宮本洋一、辻井聡、疋田貴俊、森田
    • Industrial Property Rights Holder
      岡正啓、盛山哲嗣、米田悦啓、宮本洋一、辻井聡、疋田貴俊、森田
    • Industrial Property Rights Type
      特許
    • Industrial Property Number
      2017-026027
    • Filing Date
      2017
    • Related Report
      2017 Annual Research Report

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Published: 2015-04-16   Modified: 2019-03-29  

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