| Budget Amount *help |
¥4,940,000 (Direct Cost: ¥3,800,000、Indirect Cost: ¥1,140,000)
Fiscal Year 2017: ¥1,560,000 (Direct Cost: ¥1,200,000、Indirect Cost: ¥360,000)
Fiscal Year 2016: ¥1,560,000 (Direct Cost: ¥1,200,000、Indirect Cost: ¥360,000)
Fiscal Year 2015: ¥1,820,000 (Direct Cost: ¥1,400,000、Indirect Cost: ¥420,000)
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| Outline of Final Research Achievements |
We aimed to uncover the molecular mechanism of cellular fate reprogramming during disc regeneration. We focused on two adjacent regions in wing discs, pouch and hinge, and questioned how the hinge cells are changed to pouch cells when pouch region is lost.The larval development was dietary-arrested at the end of third instar not only for using the mature wing discs that have clear pouch and hinge subdivisions, but also for long monitoring of regeneration process.
We observed that the cells in hinge region become proliferative upon pouch ablation, and the lost pouch region is eventually reconstructed. In addition, we demonstrated that the patterned expression of Wingless (Wg) that disrupted by pouch ablation was gradually reconstructed in 72 hours after ablation. These results suggest that the arrested larvae retain the ability for disc regeneration, and that the cells from hinge region most likely contribute to rebuild pouch region.
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