Investigating cellular reprogramming process during disc regeneration in Drosophila melanogaster
| Project/Area Number |
15K07075
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| Research Category |
Grant-in-Aid for Scientific Research (C)
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| Allocation Type | Multi-year Fund |
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| Section | 一般 |
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| Research Field |
Developmental biology
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| Research Institution | The University of Tokyo |
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Principal Investigator |
Tomonori Katsuyama 東京大学, 大学院薬学系研究科(薬学部), 特任助教 (70400273)
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| Research Collaborator |
KASHIO Soshiro
Zhou Mandi
FUNAKOSHI Masabumi
NISHIUCHI Kumiko
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| Project Period (FY) |
2015-04-01 – 2018-03-31
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| Project Status |
Completed (Fiscal Year 2017)
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| Budget Amount *help |
¥4,940,000 (Direct Cost: ¥3,800,000、Indirect Cost: ¥1,140,000)
Fiscal Year 2017: ¥1,560,000 (Direct Cost: ¥1,200,000、Indirect Cost: ¥360,000)
Fiscal Year 2016: ¥1,560,000 (Direct Cost: ¥1,200,000、Indirect Cost: ¥360,000)
Fiscal Year 2015: ¥1,820,000 (Direct Cost: ¥1,400,000、Indirect Cost: ¥420,000)
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| Keywords | ショウジョウバエ / 成虫原基 / 再生 / 細胞リプログラミング / 細胞運命 / 決定転換 |
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| Outline of Final Research Achievements |
We aimed to uncover the molecular mechanism of cellular fate reprogramming during disc regeneration. We focused on two adjacent regions in wing discs, pouch and hinge, and questioned how the hinge cells are changed to pouch cells when pouch region is lost.The larval development was dietary-arrested at the end of third instar not only for using the mature wing discs that have clear pouch and hinge subdivisions, but also for long monitoring of regeneration process.
We observed that the cells in hinge region become proliferative upon pouch ablation, and the lost pouch region is eventually reconstructed. In addition, we demonstrated that the patterned expression of Wingless (Wg) that disrupted by pouch ablation was gradually reconstructed in 72 hours after ablation. These results suggest that the arrested larvae retain the ability for disc regeneration, and that the cells from hinge region most likely contribute to rebuild pouch region.
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Report
(4 results)
Research Products
(10 results)
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[Journal Article] Tissue non-autonomous effects of fat body methionine metabolism on imaginal disc repair in Drosophila.2016
Author(s)
Kashio, S., Obata, F., Zhang, L., Katsuyama, T., Chihara, T., and Miura, M.
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Journal Title
Proc. Natl. Acad. Sci. USA.
Volume: 113
Issue: 7
Pages: 1835-1840
DOI
Related Report
Peer Reviewed / Open Access / Int'l Joint Research / Acknowledgement Compliant
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