Investigation into an inhibitory role of Arp5 in cardiac differentiation and reprogramming
| Project/Area Number |
15K07076
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| Research Category |
Grant-in-Aid for Scientific Research (C)
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| Allocation Type | Multi-year Fund |
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| Section | 一般 |
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| Research Field |
Developmental biology
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| Research Institution | Osaka University |
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Principal Investigator |
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| Project Period (FY) |
2015-04-01 – 2018-03-31
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| Project Status |
Completed (Fiscal Year 2017)
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| Budget Amount *help |
¥5,070,000 (Direct Cost: ¥3,900,000、Indirect Cost: ¥1,170,000)
Fiscal Year 2017: ¥1,430,000 (Direct Cost: ¥1,100,000、Indirect Cost: ¥330,000)
Fiscal Year 2016: ¥1,300,000 (Direct Cost: ¥1,000,000、Indirect Cost: ¥300,000)
Fiscal Year 2015: ¥2,340,000 (Direct Cost: ¥1,800,000、Indirect Cost: ¥540,000)
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| Keywords | 心筋分化 / 再生医療 / actin-related protein / myocardin / 心筋 / 分化 / リプログラミング |
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| Outline of Final Research Achievements |
Recently, reprogramming cellular identity is receiving increased attention in cardiovascular regenerative medicine. However, the reprogramming efficiency from non-cardiac cells to cardiac cells is not sufficiently high. In this study, I identified actin-related protein 5 (Arp5) as an inhibitory factor for cardiac differentiation. The expression level of Arp5 is significantly low in the heart tissue. Arp5 interacted with cardiovascular transcription factors myocardin and MEF2C, and it inhibited their functions. Importantly, Arp5 knockdown increased the differentiation efficiency of mouse embryonic carcinoma P19CL6 cells to cardiac cells. Thus, suppression of Arp5 expression level is probably effective measure for improvement of the cardiac reprogramming efficiency.
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Report
(4 results)
Research Products
(7 results)
