Transcriptional regulators involved in nuclear histone modification and mitochondrial gene expression
| Project/Area Number |
15K07078
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| Research Category |
Grant-in-Aid for Scientific Research (C)
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| Allocation Type | Multi-year Fund |
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| Section | 一般 |
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| Research Field |
Developmental biology
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| Research Institution | Kochi University |
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Principal Investigator |
Kawamura Kazuo 高知大学, その他部局等(名誉教授), 名誉教授 (30136361)
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| Project Period (FY) |
2015-04-01 – 2018-03-31
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| Project Status |
Completed (Fiscal Year 2017)
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| Budget Amount *help |
¥4,940,000 (Direct Cost: ¥3,800,000、Indirect Cost: ¥1,140,000)
Fiscal Year 2017: ¥1,560,000 (Direct Cost: ¥1,200,000、Indirect Cost: ¥360,000)
Fiscal Year 2016: ¥1,560,000 (Direct Cost: ¥1,200,000、Indirect Cost: ¥360,000)
Fiscal Year 2015: ¥1,820,000 (Direct Cost: ¥1,400,000、Indirect Cost: ¥420,000)
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| Keywords | ホヤ / ミトコンドリア / Tfam / YY1 / Sirtuin6 / プルダウンアッセイ / ゲルシフトアッセイ / 架橋法 / YAF2 / SIRT6 / クロスリンク法 / TFAM / ヒストン脱アセチル化 / ヒストンメチル化 / 加齢 / 出芽 / ｎｏｎ－ｃｏｄｉｎｇ－ｒｅｇｉｏｎ / 16S リボソームＲＮＡ / ＴＦＡＭ |
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| Outline of Final Research Achievements |
Aging accompanies the decrease in mitochondrial gene and respiratory activities. In the budding tunicate, Polyandrocarpa misakiensis, mitochondrial activities are repeatedly regulated during aging and budding cycles. This research project revealed first that mitochondrial transcription factor A (TFAM) acts on the D-loop of mitochondrial genome to facilitate the expression of downstream genes. Both the transcription factor, Yin-Yang-1 (YY1) and the transcriptional corepressor, Sirtuin 6 (SIRT6) are upregulated during zooidal aging. PmSirt6 mRNA, when introduced into buds together with PmYY1 mRNA, severely downregulated TFAM gene expression. Pull-down assays, gel shift assays, and cross-linking experiments indicated that YY1 recruited SIRT6 to the 5’ upstream region of TFAM gene to facilitate the deacetylation of histone H3K9. These results indicate that during normal tunicate aging, epigenetic histone modification causes the decrease of mitochondrial activity without genetic damage.
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Report
(4 results)
Research Products
(14 results)
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[Journal Article] Convergent acquisition of non-embryonic development in styelid ascidians2018
Author(s)
Ali? Alexandre、Hiebert Laurel Sky、Simion Paul、Scelzo Marta、Pr?nster Maria Mandela、Lotito Sonia、Delsuc Fr?d?ric、Douzery Emmanuel J P、Dantec Christelle、Lemaire Patrick、Darras S?bastien、Kawamura Kazuo、Brown Federico D、Tiozzo Stefano
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Journal Title
Molecular Biology and Evolution
Volume: 印刷中
Issue: 7
Pages: 1728-1743
DOI
Related Report
Peer Reviewed / Open Access / Int'l Joint Research
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