| Budget Amount *help |
¥4,940,000 (Direct Cost: ¥3,800,000、Indirect Cost: ¥1,140,000)
Fiscal Year 2017: ¥1,560,000 (Direct Cost: ¥1,200,000、Indirect Cost: ¥360,000)
Fiscal Year 2016: ¥1,560,000 (Direct Cost: ¥1,200,000、Indirect Cost: ¥360,000)
Fiscal Year 2015: ¥1,820,000 (Direct Cost: ¥1,400,000、Indirect Cost: ¥420,000)
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| Outline of Final Research Achievements |
Aging accompanies the decrease in mitochondrial gene and respiratory activities. In the budding tunicate, Polyandrocarpa misakiensis, mitochondrial activities are repeatedly regulated during aging and budding cycles. This research project revealed first that mitochondrial transcription factor A (TFAM) acts on the D-loop of mitochondrial genome to facilitate the expression of downstream genes. Both the transcription factor, Yin-Yang-1 (YY1) and the transcriptional corepressor, Sirtuin 6 (SIRT6) are upregulated during zooidal aging. PmSirt6 mRNA, when introduced into buds together with PmYY1 mRNA, severely downregulated TFAM gene expression. Pull-down assays, gel shift assays, and cross-linking experiments indicated that YY1 recruited SIRT6 to the 5’ upstream region of TFAM gene to facilitate the deacetylation of histone H3K9. These results indicate that during normal tunicate aging, epigenetic histone modification causes the decrease of mitochondrial activity without genetic damage.
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