Genetic and single-cell approaches to evaluate roles played by UPIII-Src system in frog oocyte maturation and egg fertilization
| Project/Area Number |
15K07083
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| Research Category |
Grant-in-Aid for Scientific Research (C)
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| Allocation Type | Multi-year Fund |
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| Section | 一般 |
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| Research Field |
Developmental biology
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| Research Institution | Kyoto Sangyo University |
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Principal Investigator |
SATO Ken-ichi 京都産業大学, 総合生命科学部, 教授 (30235337)
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| Research Collaborator |
TOKMAKOV Alexander A 京都産業大学, 総合生命科学部, 研究助教 (20301278)
NAKAJIMA Keisuke 広島大学, 両生類研究センター, 助手 (60260311)
IJIRI Takashi 摂南大学, 理工学部生命科学科, 講師 (20629620)
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| Project Period (FY) |
2015-04-01 – 2018-03-31
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| Project Status |
Completed (Fiscal Year 2017)
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| Budget Amount *help |
¥5,070,000 (Direct Cost: ¥3,900,000、Indirect Cost: ¥1,170,000)
Fiscal Year 2017: ¥1,820,000 (Direct Cost: ¥1,400,000、Indirect Cost: ¥420,000)
Fiscal Year 2016: ¥1,560,000 (Direct Cost: ¥1,200,000、Indirect Cost: ¥360,000)
Fiscal Year 2015: ¥1,690,000 (Direct Cost: ¥1,300,000、Indirect Cost: ¥390,000)
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| Keywords | 受精 / 卵成熟 / シグナル伝達 / チロシンリン酸化 / 細胞膜マイクロドメイン / ウロプラキンIII / ゲノム編集 / Src / 膜マイクロドメイン / アフリカツメガエル / ウロプラキン / 排卵過程の再構成 / ネッタイツメガエル / ウロプラキン３A / アポトーシス / リプログラミング / ウロプラキン３ / 卵形成 / マップキナーゼ |
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| Outline of Final Research Achievements |
We tried to prepare a uroplakin III (UPIII)-null oocyte and egg with the use of Xenopus tropicalis as a model animal, and of genome editing technologies such as TALENs and CRISPR/Cas9. At present, however, because of the highly unstable viability in UPIII-homozygously negative larva, we have not yet succeeded in obtaining females with homologous disruption in the UPIII gene loci. Therefore, we are still in the process of preparing a homozygous UPIII-null female. Under this condition, a single-cell biology approach toward oocytes and eggs that can be obtained from heterozugous UPIII-null females, such as indirect immunofluorescent experiments with the use of anti-UPIII antibody, and in vitro reconstitution of sperm-induced membrane-associated signaling events (e.g. tyrosine phosphorylation of Src) are under investigation.
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Report
(4 results)
Research Products
(21 results)
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[Presentation] Inhibition of ATP synthesis affects white spot occurrence and the increase of ATP during progesterone induced maturation in Xenopus laevis oocytes.2015
Author(s)
Ijiri, T.W., Kishikawa, J., Imamura, H., Sakiie, M., Ueno, S., Iwao, Y., Yokoyama, K., Sato, K.
Organizer
Gordon Research Conference on Fertilization and Activation of Development
Place of Presentation
アメリカ、ニューハンプシャー州、Holderness school
Year and Date
2015-07-19
Related Report
Int'l Joint Research
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[Book] Animal Models and Human Reproduction: Cell and Molecular Approaches with Reference to Human Reproduction2017
Author(s)
Sato, Constantinescu, Pence and Telugu, Mossa et al., Smorag and Pantakani, Sirard, Herrick et al., Richard et al., Mordhorst and Prather, Carnevale, Wright, Racicot and Latham, Watanabe and Tateno, La Spina et al., Kutchy et al., Nevoral and Sutovsky, Ge et al., Petkov et al., Velho et al., and Anzar
Total Pages
600
Publisher
Wiley
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