| Project/Area Number |
15K07083
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| Research Category |
Grant-in-Aid for Scientific Research (C)
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| Allocation Type | Multi-year Fund |
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| Section | 一般 |
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| Research Field |
Developmental biology
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| Research Institution | Kyoto Sangyo University |
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Principal Investigator |
SATO Ken-ichi 京都産業大学, 総合生命科学部, 教授 (30235337)
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| Research Collaborator |
TOKMAKOV Alexander A 京都産業大学, 総合生命科学部, 研究助教 (20301278)
NAKAJIMA Keisuke 広島大学, 両生類研究センター, 助手 (60260311)
IJIRI Takashi 摂南大学, 理工学部生命科学科, 講師 (20629620)
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| Project Period (FY) |
2015-04-01 – 2018-03-31
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| Project Status |
Completed (Fiscal Year 2017)
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| Budget Amount *help |
¥5,070,000 (Direct Cost: ¥3,900,000、Indirect Cost: ¥1,170,000)
Fiscal Year 2017: ¥1,820,000 (Direct Cost: ¥1,400,000、Indirect Cost: ¥420,000)
Fiscal Year 2016: ¥1,560,000 (Direct Cost: ¥1,200,000、Indirect Cost: ¥360,000)
Fiscal Year 2015: ¥1,690,000 (Direct Cost: ¥1,300,000、Indirect Cost: ¥390,000)
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| Keywords | 受精 / 卵成熟 / シグナル伝達 / チロシンリン酸化 / 細胞膜マイクロドメイン / ウロプラキンIII / ゲノム編集 / Src / 膜マイクロドメイン / アフリカツメガエル / ウロプラキン / 排卵過程の再構成 / ネッタイツメガエル / ウロプラキン３A / アポトーシス / リプログラミング / ウロプラキン３ / 卵形成 / マップキナーゼ |
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| Outline of Final Research Achievements |
We tried to prepare a uroplakin III (UPIII)-null oocyte and egg with the use of Xenopus tropicalis as a model animal, and of genome editing technologies such as TALENs and CRISPR/Cas9. At present, however, because of the highly unstable viability in UPIII-homozygously negative larva, we have not yet succeeded in obtaining females with homologous disruption in the UPIII gene loci. Therefore, we are still in the process of preparing a homozygous UPIII-null female. Under this condition, a single-cell biology approach toward oocytes and eggs that can be obtained from heterozugous UPIII-null females, such as indirect immunofluorescent experiments with the use of anti-UPIII antibody, and in vitro reconstitution of sperm-induced membrane-associated signaling events (e.g. tyrosine phosphorylation of Src) are under investigation.
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