| Budget Amount *help |
¥4,940,000 (Direct Cost: ¥3,800,000、Indirect Cost: ¥1,140,000)
Fiscal Year 2017: ¥1,560,000 (Direct Cost: ¥1,200,000、Indirect Cost: ¥360,000)
Fiscal Year 2016: ¥1,560,000 (Direct Cost: ¥1,200,000、Indirect Cost: ¥360,000)
Fiscal Year 2015: ¥1,820,000 (Direct Cost: ¥1,400,000、Indirect Cost: ¥420,000)
|
|---|
| Outline of Final Research Achievements |
To investigate the correlation between hypoxia and embryonic development, we focused on the anaerobic glycolysis, the cellular metabolism under hypoxia. Whole-embryo culture of mid-gestation embryos with glycolytic inhibitors caused neural tube closure defects at cranial levels without cell death. Therefore, anaerobic glycolytic activity seems to be essential for neural plate bending associated with cell shape changes. Consistent with this, we observed suppression of apical constriction and reduction of phosphorylated myosin light chain 2, activator of actin contraction in neuroepithelial cells of inhibitor-treated embryos. Our gene expression analyses demonstrated that some hypoxia-responsive glycolytic enzyme genes were specifically expressed in neural plate of mid-gestation embryos. Taken together, intrauterine hypoxic condition regulates neural plate bending via tissue-specific activation of glycolysis.
|
