Elucidation of the mechanism of photosynthetic electron transport-dependent transcriptional regulation mediated by thioredoxin

• Project/Area Number: 15K07096
• Research Category: Grant-in-Aid for Scientific Research (C)
• Allocation Type: Multi-year Fund
• Section: 一般
• Research Field: Plant molecular biology/Plant physiology
• Research Institution: Saitama University
• Principal Investigator: Hihara Yukako 埼玉大学, 理工学研究科, 教授 (60323375)
• Project Period (FY): 2015-04-01 – 2019-03-31
• Project Status: Completed (Fiscal Year 2018)
• Budget Amount: ¥4,940,000 (Direct Cost: ¥3,800,000、Indirect Cost: ¥1,140,000); Fiscal Year 2017: ¥1,560,000 (Direct Cost: ¥1,200,000、Indirect Cost: ¥360,000); Fiscal Year 2016: ¥1,560,000 (Direct Cost: ¥1,200,000、Indirect Cost: ¥360,000); Fiscal Year 2015: ¥1,820,000 (Direct Cost: ¥1,400,000、Indirect Cost: ¥420,000)
• Keywords: 転写制御 / レドックス制御 / シアノバクテリア / 光合成 / チオレドキシン / 環境応答 / 転写因子 / シグナル伝達 / 光合成電子伝達

Outline of Final Research Achievements

We performed biochemical and physiological characterization of two transcription factors in Synechocystis sp. PCC 6803, RpaB and Sll1961, which were previously identified as interacting partners of thioredoxin. As for RpaB, we performed genome-wide search of its target genes and analysis of regulatory mechanism of its DNA binding activity dependent on redox state of photosynthetic electron transport chain. As for Sll1961, we identified changes in its thiol-redox state upon interaction with thioredoxin. Moreover, we successfully identified target genes of Sll1961 from the results of RNA-seq analysis.

Academic Significance and Societal Importance of the Research Achievements

本研究では、チオレドキシンとの相互作用により、光合成に依存して働くと考えられる２つの転写因子RpaBとSll1961について、その標的遺伝子および活性制御機構の一端を明らかにした。光合成に依存した遺伝子発現制御のメカニズムは、シアノバクテリアのみならず、他の光合成生物においても未解明な部分が多く、本研究で得られた成果は、転写レベルで環境応答を調節する応用技術につながるなど、植物科学全体の発展に貢献すると期待される。

Research Products

• [Int'l Joint Research] University of Freiburg(ドイツ)
• [Journal Article] Biocomputational analyses and experimental validation identify the complex regulon controlled by the redox-responsive transcription factor RpaB. (2019)
  • Author(s): Riediger M, Kadowaki T, Nagayama R, Georg G, Hihara Y, Hess WR
  • Journal Title: iScience Volume: 印刷中 Pages: 316-331
  • DOI: 10.1016/j.isci.2019.04.033
  • Peer Reviewed / Open Access / Int'l Joint Research
• [Journal Article] Interaction of the GntR-family transcription factor Sll1961 with thioredoxin in the cyanobacterium Synechocystis sp. PCC 6803 (2018)
  • Author(s): Kujirai, J., Nanba, S., Kadowaki, T., Oka, Y., Nishiyama, Y., Hayashi, Y., Arai, M. and Hihara, Y.
  • Journal Title: Sci. Rep. Volume: 8 Issue: 1 Pages: 6666-6666
  • DOI: 10.1038/s41598-018-25077-5
  • Peer Reviewed / Open Access
• [Journal Article] Effects of inactivation of the cyAbrB2 transcription factor together with glycogen synthesis on cellular metabolism and free fatty acid production in the cyanobacterium Synechocystis sp. PCC 6803 (2018)
  • Author(s): Yuta Kodama, Akihito Kawahara, Atsuko Miyagi, Toshiki Ishikawa, Maki Kawai-Yamada, Yasuko Kaneko, Yasushi Takimura and Yukako Hihara
  • Journal Title: Biotechnology and Bioengineering Volume: 115 Issue: 12 Pages: 2974-2985
  • DOI: 10.1002/bit.26842
  • Peer Reviewed
• [Journal Article] From cyanobacteria and algae to land plants: The RpaB/Ycf27 regulatory network in transition. (2018)
  • Author(s): Riediger M, Hihara Y, Hess WR
  • Journal Title: Perspectives in Phycology Volume: 5 Issue: 1 Pages: 13-25
  • DOI: 10.1127/pip/2018/0078
  • Peer Reviewed / Open Access / Int'l Joint Research
• [Journal Article] Oxidation of Translation Factor EF-Tu Inhibits the Repair of Photosystem II. (2018)
  • Author(s): Jimbo H, Yutthanasirikul R, Nagano T, Hisabori T, Hihara Y, Nishiyama Y
  • Journal Title: Plant Physiology Volume: 176 Issue: 4 Pages: 2691-2699
  • DOI: 10.1104/pp.18.00037
  • Peer Reviewed
• [Journal Article] A feed-forward loop consisting of the response regulator RpaB and the small RNA PsrR1 controls light acclimation of photosystem I gene expression in the cyanobacterium Synechocystis sp. PCC 6803 (2016)
  • Author(s): Kadowaki, T., Nagayama, R., Georg, J., Nishiyama, Y., Wilde, A., Hess, W. and Hihara, Y.
  • Journal Title: Plant Cell Physiol. Volume: 57(4) Issue: 4 Pages: 813-23
  • DOI: 10.1093/pcp/pcw028
  • Peer Reviewed / Int'l Joint Research
• [Journal Article] Free fatty acid production in the cyanobacterium Synechocystis sp. PCC 6803 is enhanced by deletion of the cyAbrB2 transcriptional regulator. (2016)
  • Author(s): Kawahara A, Sato Y, Saito Y, Kaneko Y, Takimura Y, Hagihara H, Hihara Y.
  • Journal Title: Journal of Biotechnology Volume: 220 Pages: 1-11
  • DOI: 10.1016/j.jbiotec.2015.12.035
  • Peer Reviewed
• [Journal Article] Transcriptional and posttranscriptional regulation of cyanobacterial photosynthesis. (2016)
  • Author(s): Wilde A, Hihara Y.
  • Journal Title: Biochimica et Biophysica Acta Volume: 1857 Issue: 3 Pages: 296-308
  • DOI: 10.1016/j.bbabio.2015.11.002
  • Peer Reviewed / Int'l Joint Research / Acknowledgement Compliant
• [Journal Article] RNA-seq Profiling Reveals Novel Target Genes of LexA in the Cyanobacterium Synechocystis sp. PCC 6803. (2016)
  • Author(s): Kizawa A, Kawahara A, Takimura Y, Nishiyama Y, Hihara Y.
  • Journal Title: Frontiers in Microbiology Volume: 7 Pages: 193-193
  • DOI: 10.3389/fmicb.2016.00193
  • Peer Reviewed / Open Access
• [Journal Article] Identification of OmpR-family response regulators interacting with thioredoxin in the cyanobacterium Synechocystis sp. PCC 6803 (2015)
  • Author(s): Kadowaki, T., Nishiyama, Y., Hisabori, T. and Hihara, Y.
  • Journal Title: PLos One Volume: 10(3) Issue: 3 Pages: e0119107-e0119107
  • DOI: 10.1371/journal.pone.0119107
  • Peer Reviewed / Open Access
• [Journal Article] Analysis of spontaneous suppressor mutants from the photomixotrophically-grown pmgA-disrupted mutant in the cyanobacterium Synechocystis sp. PCC 6803 (2015)
  • Author(s): Nishijima, Y., Kanesaki, Y., Yoshikawa, H., Ogawa, T., Sonoike, K., Nishiyama, Y. and Hihara, Y.
  • Journal Title: Photosynth. Res. Volume: - Issue: 2-3 Pages: 465-475
  • DOI: 10.1007/s11120-015-0143-8
  • Peer Reviewed
• [Presentation] シアノバクテリアSynechocystis sp. PCC 6803におけるGntR型転写因子Sll1961の機能解析 (2018)
  • Author(s): 難波 理、鯨井 純一、門脇 太朗、岡 芳樹、西山 佳孝、林 勇樹、新井 宗仁、日原 由香子
  • Organizer: 第17回微生物研究会
• [Presentation] シアノバクテリアSynechocystis sp. PCC 6803 における GntR型転写因子Sll1961の機能解析 (2018)
  • Author(s): 難波 理 、鯨井 純一、岡 芳樹、林 勇樹、新井 宗仁 、園池 公毅、 日原 由香子
  • Organizer: 第13回日本ゲノム微生物学会年会
• [Presentation] Identification of transcription factors interacting with thioredoxin in the cyanobacterium Synechocystis sp. PCC 6803 (2018)
  • Author(s): Yukako Hihara
  • Organizer: CLS, Tokyo Tech. International Forum 2018 "Redox regulation of protein functions, transcription, translation and folding
  • Int'l Joint Research / Invited
• [Presentation] シアノバクテリアSynechocystis sp. PCC 6803ゲノムに おける転写因子RpaB標的遺伝子の同定 (2017)
  • Author(s): 永山竜太、門脇太朗、Matthias Riediger、Wolfgang Hess、日原由香子
  • Organizer: 第58回日本植物生理学会年会
  • Place of Presentation: 鹿児島大学
  • Year and Date: 2017-03-16
• [Presentation] 転写制御因子のレドックス制御 －vitroから vivoへの遠い道のり－ (2017)
  • Author(s): 日原由香子
  • Organizer: 藍藻の分子生物学2017
  • Invited
• [Presentation] A feed-forward loop consisting of the response regulator RpaB and the small RNA PsrR1 controls light acclimation of photosystem I gene expression in the cyanobacterium Synechocystis sp. PCC 6803. (2016)
  • Author(s): Yukako Hihara
  • Organizer: 7th International Conference Photosynthesis Research for Sustainability - 2016
  • Place of Presentation: ロシア・Pushchino
  • Year and Date: 2016-06-09
  • Int'l Joint Research / Invited
• [Presentation] 転写因子RpaBと低分子RNA PsrR1による光化学系Ⅰ遺伝子の発現制御 -Feed-Forward Loopの形成- (2016)
  • Author(s): 門脇太朗、永山竜太、Jens Georg、Annegret Wilde、Wolfgang R.Hess、日原由香子
  • Organizer: 第7回日本光合成学会
  • Place of Presentation: 東京理科大学葛飾キャンパス
  • Year and Date: 2016-05-27
• [Presentation] シアノバクテリアSynechocystis sp. PCC6803におけるRpaB/PsrR1システムによ る光化学系I遺伝子の強光応答の二元的制御 (2016)
  • Author(s): 永山竜太、門脇太朗、Jens Georg、Annegret Wilde、Wolfgang Hess、日原由香子
  • Organizer: 日本植物生理学会第57回大会
  • Place of Presentation: 岩手大学上田キャンパス（岩手県盛岡市）
  • Year and Date: 2016-03-18
• [Presentation] シアノバクテリアSynechocystis sp. PCC 6803における転写因子 Sll1961とチオレドキシンとの相互作用解析 (2016)
  • Author(s): 鯨井純一、門脇太朗、新井宗仁、久堀徹、日原由香子
  • Organizer: 日本植物生理学会第57回大会
  • Place of Presentation: 岩手大学上田キャンパス（岩手県盛岡市）
  • Year and Date: 2016-03-18
• [Presentation] Photosynthesis-dependent transcriptional regulation in cyanobacteria (2015)
  • Author(s): Yukako Hihara
  • Organizer: 15th International Symposium on Photosynthetic Prokaryotes
  • Place of Presentation: チュービンゲン大学（ドイツ）
  • Year and Date: 2015-08-02
  • Int'l Joint Research / Invited
• [Presentation] Elucidation of mechanisms of transcriptional regulation dependent on thioredoxin in the cyanobacterium Synechocystis sp. PCC 6803 (2015)
  • Author(s): Taro Kadowaki, Yoshitaka Nishiyama, Toru Hisabori, Yukako Hihara
  • Organizer: 15th International Symposium on Photosynthetic Prokaryotes
  • Place of Presentation: チュービンゲン大学（ドイツ）
  • Year and Date: 2015-08-02
  • Int'l Joint Research
• [Presentation] Synechocystis sp.PCC 6803 における光化学系Ⅰ遺伝子の強光応答の二元的制御システム (2015)
  • Author(s): 永山竜太、門脇太朗、日原由香子
  • Organizer: 第6回日本光合成学会
  • Place of Presentation: 岡山国際交流センター（岡山県岡山市）
  • Year and Date: 2015-05-22
• [Presentation] シアノバクテリアSynechocystis sp.PCC 6803 におけるチオレドキシンと相互作用する転写因子の探索 (2015)
  • Author(s): 門脇太朗、西山佳孝、久堀徹、日原由香子
  • Organizer: 第6回日本光合成学会
  • Place of Presentation: 岡山国際交流センター（岡山県岡山市）
  • Year and Date: 2015-05-22