Mechanism of the acclimation of photosystem II repair to strong light

• Project/Area Number: 15K07097
• Research Category: Grant-in-Aid for Scientific Research (C)
• Allocation Type: Multi-year Fund
• Section: 一般
• Research Field: Plant molecular biology/Plant physiology
• Research Institution: Saitama University
• Principal Investigator: Nishiyama Yoshitaka 埼玉大学, 理工学研究科, 教授 (30281588)
• Project Period (FY): 2015-04-01 – 2018-03-31
• Project Status: Completed (Fiscal Year 2017)
• Budget Amount: ¥5,070,000 (Direct Cost: ¥3,900,000、Indirect Cost: ¥1,170,000); Fiscal Year 2017: ¥1,560,000 (Direct Cost: ¥1,200,000、Indirect Cost: ¥360,000); Fiscal Year 2016: ¥1,690,000 (Direct Cost: ¥1,300,000、Indirect Cost: ¥390,000); Fiscal Year 2015: ¥1,820,000 (Direct Cost: ¥1,400,000、Indirect Cost: ¥420,000)
• Keywords: 光合成 / 強光ストレス / タンパク質合成 / 翻訳因子 / 環境 / ストレス / 蛋白質 / 遺伝子

Outline of Final Research Achievements

Photosystem II (PSII) is susceptible to inactivation under strong light, and this phenomenon is referred to as photoinhibition of PSII. In the present study, we found that oxidation of translation factor EF-Tu is associated with photoinhibition of PSII in cyanobacterium Synechocystis sp. PCC 6803. Under strong light, reactive oxygen species, which are generated from the photosynthetic machinery, oxidize a specific cysteine residue of EF-Tu and inactivate its function, resulting in the suppression of protein synthesis and inhibition of the repair of PSII. When photosynthetic cells acclimate to strong light, PSII becomes more resistant to photoinhibtion. We found that increased levels of EF-Tu play an important role in the acclimation of PSII to strong light, by protecting protein synthesis from oxidative stress and by enhancing the repair of PSII.

Research Products

• [Int'l Joint Research] University of California, Berkeley(米国)
• [Int'l Joint Research] King Monkut's University of Technology(Thailand)
• [Journal Article] ATP is a driving force in the repair of photosystem II during photoinhibition (2018)
  • Author(s): Murata, N. and Nishiyama, Y.
  • Journal Title: Plant Cell Environ. Volume: 41 Issue: 2 Pages: 285-299
  • DOI: 10.1111/pce.13108
  • Peer Reviewed
• [Journal Article] Oxidation of Translation Factor EF-Tu Inhibits the Repair of Photosystem II. (2018)
  • Author(s): Jimbo H, Yutthanasirikul R, Nagano T, Hisabori T, Hihara Y, Nishiyama Y
  • Journal Title: Plant Physiology Volume: 176 Issue: 4 Pages: 2691-2699
  • DOI: 10.1104/pp.18.00037
  • Peer Reviewed
• [Journal Article] Interaction of the GntR-family transcription factor Sll1961 with thioredoxin in the cyanobacterium Synechocystis sp. PCC 6803 (2018)
  • Author(s): Kujirai, J., Nanba, S., Kadowaki, T., Oka, Y., Nishiyama, Y., Hayashi, Y., Arai, M. and Hihara, Y.
  • Journal Title: Sci. Rep. Volume: 8 Issue: 1 Pages: 6666-6666
  • DOI: 10.1038/s41598-018-25077-5
  • Peer Reviewed / Open Access
• [Journal Article] Adverse effects of strong light and nitrogen deficiency cell viability, photosynthesis, and motility of the red-tide dinoflagellate Karenia mikimotoi (2018)
  • Author(s): Yuasa, K., Shikata, T., Kuwahara, Y. and Nishiyama, Y.
  • Journal Title: Phycologia Volume: 57(4) Issue: 5 Pages: 525-533
  • DOI: 10.2216/17-61.1
  • Peer Reviewed
• [Journal Article] The LexA transcription factor regulates fatty acid biosynthetic genes in the cyanobacterium Synechocystis sp. PCC 6803 (2017)
  • Author(s): Kizawa, A., Kawahara, A., Takashima, K., Takimura, Y., Nishiyama, Y. and Hihara, Y.
  • Journal Title: Plant J. Volume: 92(2) Issue: 2 Pages: 189-198
  • DOI: 10.1111/tpj.13644
  • Peer Reviewed
• [Journal Article] Disturbance of cell-size determination by forced overproduction of sulfoquinovosyl diacylglycerol in the cyanobacterium Synechococcus elongatus PCC 7942 (2017)
  • Author(s): Sato, N., Ebiya, Y., Kobayashi, R., Nishiyama, Y. and Tsuzuki, M.
  • Journal Title: Biochem. Biophy. Res. Commun. Volume: 487 Issue: 3 Pages: 734-739
  • DOI: 10.1016/j.bbrc.2017.04.129
  • Peer Reviewed
• [Journal Article] Overexpressed superoxide dismutase and catalase act synergistically to protect the repair of PSII during photoinhibition in Synechococcus elongatus PCC 7942 (2016)
  • Author(s): Sae-Tang, P., Hihara, Y., Yumoto, I., Orikasa, Y., Okuyama, H. and Nishiyama, Y.
  • Journal Title: Plant Cell Physiol. Volume: 57 Issue: 9 Pages: 1899-1907
  • DOI: 10.1093/pcp/pcw110
  • Peer Reviewed / Int'l Joint Research / Acknowledgement Compliant
• [Journal Article] Moderate heat stress stimulates repair of photosystem II during photoinhibition in Synechocystis sp. PCC 6803 (2016)
  • Author(s): Ueno, M., Sae-Tang, P., Kusama, Y., Hihara, Y., Matsuda, M., Hasunuma, T. and Nishiyama, Y.
  • Journal Title: Plant Cell Physiol. Volume: 57 Issue: 11 Pages: 2417-2426
  • DOI: 10.1093/pcp/pcw153
  • Peer Reviewed / Int'l Joint Research / Acknowledgement Compliant
• [Journal Article] Rapid acquisition of high-affinity DNA aptamer motifs recognizing microbial cell surfaces using polymer-enhanced capillary transient isotachophoresis (2016)
  • Author(s): Saito, S., Hirose, K., Tsuchida, M., Wakui, K., Yoshimoto, K., Nishiyama, Y. and Shibukawa, M.
  • Journal Title: Chem. Commun. Volume: 52(3) Issue: 3 Pages: 461-4
  • DOI: 10.1039/c5cc07268a
  • Peer Reviewed / Open Access
• [Journal Article] Oxidation of a cysteine residue in elongation factor EF-Tu reversibly inhibits translation in the cyanobacterium Synechocystis sp. PCC 6803 (2016)
  • Author(s): Yutthanasirikul, R., Nagano, T., Jimbo, H., Hihara, Y., Kanamori, T., Ueda, T., Haruyama, T., Konno, H., Yoshida, K., Hisabori, T. and Nishiyama, Y.
  • Journal Title: J. Biol. Chem. Volume: 291(11) Issue: 11 Pages: 5860-5870
  • DOI: 10.1074/jbc.m115.706424
  • Peer Reviewed / Open Access / Acknowledgement Compliant
• [Journal Article] A feed-forward loop consisting of the response regulator RpaB and the small RNA PsrR1 controls light acclimation of photosystem I gene expression in the cyanobacterium Synechocystis sp. PCC 6803 (2016)
  • Author(s): Kadowaki, T., Nagayama, R., Georg, J., Nishiyama, Y., Wilde, A., Hess, W. and Hihara, Y.
  • Journal Title: Plant Cell Physiol. Volume: 57(4) Issue: 4 Pages: 813-23
  • DOI: 10.1093/pcp/pcw028
  • Peer Reviewed / Int'l Joint Research
• [Journal Article] RNA-seq Profiling Reveals Novel Target Genes of LexA in the Cyanobacterium Synechocystis sp. PCC 6803. (2016)
  • Author(s): Kizawa A, Kawahara A, Takimura Y, Nishiyama Y, Hihara Y.
  • Journal Title: Frontiers in Microbiology Volume: 7 Pages: 193-193
  • DOI: 10.3389/fmicb.2016.00193
  • Peer Reviewed / Open Access
• [Journal Article] Light spectrum regulates cell accumulation during daytime in the raphidophyte Chattonella antiqua causing noxious red tides (2016)
  • Author(s): Shikata, T., Matsunaga, S., Kuwahara, Y., Iwahori, S. and Nishiyama, Y.
  • Journal Title: J. Photochem. Photobiol. B Biol. Volume: 160 Pages: 128-133
  • DOI: 10.1016/j.jphotobiol.2016.03.046
  • Peer Reviewed
• [Journal Article] Zeaxanthin and echinenone protect the repair of photosystem II from inhibition by singlet oxygen in Synechocystis sp. PCC 6803 (2015)
  • Author(s): Kusama, Y., Inoue, S., Jimbo, H., Takaichi, S., Sonoike, K., Hihara, Y. and Nishiyama, Y.
  • Journal Title: Plant Cell Physiol. Volume: 56(5) Issue: 5 Pages: 906-916
  • DOI: 10.1093/pcp/pcv018
  • Peer Reviewed / Acknowledgement Compliant
• [Journal Article] Oxidation of translation factor EF-G transiently retards the translational elongation cycle in Escherichia coli (2015)
  • Author(s): Nagano, T., Yutthanasirikul, R., Hihara, Y., Hisabori, T., Kanamori, T., Takeuchi, N., Ueda, T. and Nishiyama, Y.
  • Journal Title: J. Biochem. Volume: - Issue: 2 Pages: 165-72
  • DOI: 10.1093/jb/mvv026
  • Peer Reviewed / Open Access / Acknowledgement Compliant
• [Journal Article] Identification of OmpR-family response regulators interacting with thioredoxin in the cyanobacterium Synechocystis sp. PCC 6803 (2015)
  • Author(s): Kadowaki, T., Nishiyama, Y., Hisabori, T. and Hihara, Y.
  • Journal Title: PLos One Volume: 10(3) Issue: 3 Pages: e0119107-e0119107
  • DOI: 10.1371/journal.pone.0119107
  • Peer Reviewed / Open Access
• [Journal Article] Analysis of spontaneous suppressor mutants from the photomixotrophically-grown pmgA-disrupted mutant in the cyanobacterium Synechocystis sp. PCC 6803 (2015)
  • Author(s): Nishijima, Y., Kanesaki, Y., Yoshikawa, H., Ogawa, T., Sonoike, K., Nishiyama, Y. and Hihara, Y.
  • Journal Title: Photosynth. Res. Volume: - Issue: 2-3 Pages: 465-475
  • DOI: 10.1007/s11120-015-0143-8
  • Peer Reviewed
• [Presentation] Redox regulation of translation and stress response of photosynthesis (2018)
  • Author(s): Nishiyama, Y.
  • Organizer: Tokyo Tech CLS International Forum 2018
  • Int'l Joint Research / Invited
• [Presentation] Enhanced expression of translation factor EF-Tu accelerates the repair of photosystem II during acclimation to strong light in Synechocystis sp. PCC 6803 (2018)
  • Author(s): Jimbo, H., Izuhara, T., Nishiyama, Y.
  • Organizer: Tokyo Tech CLS International Forum 2018
• [Presentation] Characterization of PGRL1 in photoprotection of PSI in the green alga Chlamydomonas reinhardtii (2018)
  • Author(s): Takahashi, H., Nishiyama, Y.
  • Organizer: Tokyo Tech CLS International Forum 2018
• [Presentation] Redox regulation of translation and high-light response of photosynthesis (2018)
  • Author(s): Nishiyama, Y.
  • Organizer: Department Seminar, University of California, Berkeley
  • Int'l Joint Research / Invited
• [Presentation] 赤潮藻類Karenia mikimotoiの生残、光合成、遊泳に及ぼす貧栄養と強光の影響 (2018)
  • Author(s): 湯浅光貴、紫加田知幸、西山佳孝
  • Organizer: 平成30年度日本水産学会年会
• [Presentation] シロイヌナズナの光化学系II光阻害における葉緑体翻訳因子 EF-Tu の役割 (2018)
  • Author(s): 新庄梓、神保晴彦、熊木裕香、西山佳孝
  • Organizer: 第59回日本植物生理学会年会
• [Presentation] Chlamydomonas reinhardtii の PSI 光防御における PGRL1 の役割 (2018)
  • Author(s): 髙橋拓子、西山佳孝
  • Organizer: 第59回日本植物生理学会年会
• [Presentation] Synechocystis sp. PCC 6803の強光順化におけるPSII修復能力と翻訳因子EF-Tuの役割 (2018)
  • Author(s): 神保晴彦、出原太智、西山佳孝
  • Organizer: 第59回日本植物生理学会年会
• [Presentation] シアノバクテリアSynechocystis sp. PCC 6803 の超強光下における生存戦略 (2018)
  • Author(s): 出原太智、神保晴彦、高市真一、西山佳孝
  • Organizer: 第59回日本植物生理学会年会
• [Presentation] シアノバクテリア光化学系IIの修復における熱ショックタンパク質DnaK3の役割 (2017)
  • Author(s): 河村大介、渡辺智、吉川博文、西山佳孝
  • Organizer: 第58回日本植物生理学会年会
  • Place of Presentation: 鹿児島大学、鹿児島県鹿児島市
  • Year and Date: 2017-03-18
• [Presentation] シロイヌナズナ葉緑体翻訳因子EF-Tuの酸化傷害の分子機構 (2017)
  • Author(s): 熊木裕香、小林達巧、西山佳孝
  • Organizer: 第58回日本植物生理学会年会
  • Place of Presentation: 鹿児島大学、鹿児島県鹿児島市
  • Year and Date: 2017-03-18
• [Presentation] シアノバクテリア光化学系IIの光防御機構におけるオレンジカロテノイドプロテインの役割 (2017)
  • Author(s): 高橋拓子、草間友里、李新祥、高市真一、伊藤繁、山川伯壽、西山佳孝
  • Organizer: 第58回日本植物生理学会年会
  • Place of Presentation: 鹿児島大学、鹿児島県鹿児島市
  • Year and Date: 2017-03-17
• [Presentation] 緑藻クラミドモナスの葉緑体光応答におけるMAPキナーゼの役割 (2017)
  • Author(s): 神保晴彦、西山佳孝、Krishna K. Niyogi
  • Organizer: 第58回日本植物生理学会年会
  • Place of Presentation: 鹿児島大学、鹿児島県鹿児島市
  • Year and Date: 2017-03-16
• [Presentation] 赤潮渦鞭毛藻Karenia mikimotoiの光合成に対する強光と貧栄養条件の影響 (2017)
  • Author(s): 湯浅光貴、紫加田知幸、西山佳孝
  • Organizer: 第58回日本植物生理学会年会
  • Place of Presentation: 鹿児島大学、鹿児島県鹿児島市
  • Year and Date: 2017-03-16
• [Presentation] 翻訳因子EF-Tuの酸化傷害と光化学系IIの修復阻害の関係 (2017)
  • Author(s): 神保晴彦、Rayakorn Yutthanasirikul、西山佳孝
  • Organizer: 第8回日本光合成学会年会
• [Presentation] 緑藻クラミドモナスのPSI光防御におけるPGRL1の役割 (2017)
  • Author(s): 高橋拓子、曽根和樹、西山佳孝
  • Organizer: 第8回日本光合成学会年会
• [Presentation] 光合成におけるカロテノイドの機能解析 (2017)
  • Author(s): 西山佳孝
  • Organizer: 日本植物学会第81回大会
  • Invited
• [Presentation] 翻訳因子のレドックス制御と光合成の強光応答 (2017)
  • Author(s): 西山佳孝
  • Organizer: 藍藻の分子生物学2017
  • Invited
• [Presentation] Synechocystis sp. PCC 6803 における光化学系IIの強光順化機構 (2017)
  • Author(s): 神保晴彦、出原太智、西山佳孝
  • Organizer: 藍藻の分子生物学2017
• [Presentation] シアノバクテリア光化学系IIの修復過程におけるオレンジカロテノイドプロテインの役割 (2017)
  • Author(s): 高橋拓子、李新祥、山川伯壽、高市真一、伊藤繁、西山佳孝
  • Organizer: 藍藻の分子生物学2017
• [Presentation] 光合成の環境応答におけるタンパク質合成系のレドックス制御の役割 (2016)
  • Author(s): 西山佳孝
  • Organizer: ダイナミックアライアンスG3分科会
  • Place of Presentation: 北海道大学、北海道札幌市
  • Year and Date: 2016-12-02
• [Presentation] Effects of temperature stress on photoinhibition of photosystem II in Synechocystis sp. PCC 6803 (2016)
  • Author(s): Nishiyama, Y.
  • Organizer: Finnish-Japanese Symposium 2016
  • Place of Presentation: Saariselka, Finland
  • Year and Date: 2016-09-06
  • Int'l Joint Research / Invited
• [Presentation] Roles of the overexpression of orange carotenoid protein in the protection of photosystem II against photoinhibition in Synechocystis sp. PCC 6803 (2016)
  • Author(s): Takahashi, H., Kusama, Y., Takaichi, S., Itoh, S., Yamakawa, H. and Nishiyama, Y.
  • Organizer: Finnish-Japanese Symposium 2016
  • Place of Presentation: Saariselka, Finland
  • Year and Date: 2016-09-06
  • Int'l Joint Research / Invited
• [Presentation] Redox regulation of the repair of photosystem II under photoinhibition (2016)
  • Author(s): Nishiyama, Y.
  • Organizer: The 17th International Photosynthesis Congress
  • Place of Presentation: Maastricht, The Netherlands
  • Year and Date: 2016-08-11
  • Int'l Joint Research / Invited
• [Presentation] 光合成システムの強光応答 (2016)
  • Author(s): 西山佳孝
  • Organizer: 分子研 CIMoSセミナー
  • Place of Presentation: 分子科学研究所、愛知県岡崎市
  • Year and Date: 2016-03-28
  • Invited
• [Presentation] シロイヌナズナ葉緑体翻訳因子EF-GとEF-Tuの酸化ストレス応答 (2016)
  • Author(s): 熊木裕香、濱川菜桜、米山拓、西山佳孝
  • Organizer: 第57回日本植物生理学会年会
  • Place of Presentation: 岩手大学、岩手県盛岡市
  • Year and Date: 2016-03-18
• [Presentation] 赤潮藻類における光化学系IIの強光と高温ストレスに対する応答 (2016)
  • Author(s): 桑原悠輔、紫加田知幸、西山佳孝
  • Organizer: 第57回日本植物生理学会年会
  • Place of Presentation: 岩手大学、岩手県盛岡市
  • Year and Date: 2016-03-18
• [Presentation] Synergistic effects of iron superoxide dismutase and catalase on the protection of photosynthesis to strong light in Synechococcus elongatus PCC 7942 (2016)
  • Author(s): Penporn Sae-Tang, Yoshitaka Nishiyama
  • Organizer: 第57回日本植物生理学会年会
  • Place of Presentation: 岩手大学、岩手県盛岡市
  • Year and Date: 2016-03-18
• [Presentation] 微細藻類における強光阻害現象 (2016)
  • Author(s): 西山佳孝
  • Organizer: 日本プランクトン学会シンポジウム「光環境を巡る植物プランクトンの生理生態学最前線」
  • Place of Presentation: 東京大学、東京都
  • Year and Date: 2016-03-14
  • Invited
• [Presentation] Regulation and protection of the repair of photosystem II under oxidative stress (2015)
  • Author(s): Yoshitaka Nishiyama
  • Organizer: Yamada Conference “International Symposium on Dynamics and Regulation of Photosynthesis”
  • Place of Presentation: 奈良春日野国際フォーラム、奈良県奈良市
  • Year and Date: 2015-10-29
  • Int'l Joint Research / Invited
• [Presentation] Photo-oxidative stress to photosynthesis: a new mechanism for photoinhibition of PSII and roles of reactive oxygen species (2015)
  • Author(s): Yoshitaka Nishiyama
  • Organizer: Special Seminar, King’s Mongkut University of Technology
  • Place of Presentation: King’s Mongkut University of Technology, Thonburi, Bangkok, Thailand
  • Year and Date: 2015-09-16
  • Int'l Joint Research / Invited
• [Remarks] 環境応答研究室
  • URL: http://park.saitama-u.ac.jp/~kankyo/index.html