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Identification and functional characterization of protein kinases mediating blue light-dependent stomatal opening

Research Project

Project/Area Number 15K07101
Research Category

Grant-in-Aid for Scientific Research (C)

Allocation TypeMulti-year Fund
Section一般
Research Field Plant molecular biology/Plant physiology
Research InstitutionNagoya University

Principal Investigator

Inoue Shin-ichiro  名古屋大学, 理学研究科, 助教 (40532693)

Research Collaborator HAYASHI Maki  
OKUMURA Masaki  
Project Period (FY) 2015-04-01 – 2018-03-31
Project Status Completed (Fiscal Year 2017)
Budget Amount *help
¥5,200,000 (Direct Cost: ¥4,000,000、Indirect Cost: ¥1,200,000)
Fiscal Year 2017: ¥1,170,000 (Direct Cost: ¥900,000、Indirect Cost: ¥270,000)
Fiscal Year 2016: ¥1,170,000 (Direct Cost: ¥900,000、Indirect Cost: ¥270,000)
Fiscal Year 2015: ¥2,860,000 (Direct Cost: ¥2,200,000、Indirect Cost: ¥660,000)
Keywords気孔 / 青色光 / フォトトロピン / プロテインキナーゼ / 気孔開口 / カリウムチャネル
Outline of Final Research Achievements

Stomata open in response to blue light, and the opening is mediated by a phototropin-activated proton pump and a consequent potassium uptake in stomatal guard cells. However, signaling for the stomatal opening is not fully understood. In this study, we performed a protein-protein interaction screening and identified CIPK23 as a phototropin interactor. As the results of functional analyses of CIPK23, we found that CIPK23 mediates stomatal opening not through the proton pump activation but through the potassium channel activation in guard cells.

Report

(4 results)
  • 2017 Annual Research Report   Final Research Report ( PDF )
  • 2016 Research-status Report
  • 2015 Research-status Report
  • Research Products

    (21 results)

All 2018 2017 2016 2015 Other

All Int'l Joint Research (7 results) Journal Article (9 results) (of which Int'l Joint Research: 5 results,  Peer Reviewed: 9 results,  Open Access: 8 results,  Acknowledgement Compliant: 4 results) Presentation (5 results) (of which Int'l Joint Research: 1 results)

  • [Int'l Joint Research] University of Glasgow(United Kingdom)

    • Related Report
      2017 Annual Research Report
  • [Int'l Joint Research] Henan University(China)

    • Related Report
      2017 Annual Research Report
  • [Int'l Joint Research] University of Copenhagen(Denmark)

    • Related Report
      2017 Annual Research Report
  • [Int'l Joint Research] University of Gasgow(United Kingdom)

    • Related Report
      2016 Research-status Report
  • [Int'l Joint Research] Henan University(China)

    • Related Report
      2016 Research-status Report
  • [Int'l Joint Research] University of Glasgow(United Kingdom)

    • Related Report
      2015 Research-status Report
  • [Int'l Joint Research] Henan University(China)

    • Related Report
      2015 Research-status Report
  • [Journal Article] Functional characterization of a constitutively active kinase variant of Arabidopsis phototropin 1.2017

    • Author(s)
      Peterson, J., Inoue, S., Kelly, S. M., Sullivan, S., Kinoshita, T., Christie, J. M.
    • Journal Title

      J. Biol. Chem.

      Volume: 292 Issue: 33 Pages: 13843-13852

    • DOI

      10.1074/jbc.m117.799643

    • Related Report
      2017 Annual Research Report
    • Peer Reviewed / Open Access / Int'l Joint Research
  • [Journal Article] Blue light regulation of stomatal opening and the plasma membrane H+-ATPase.2017

    • Author(s)
      Inoue S, Kinoshita T.
    • Journal Title

      Plant Physiology

      Volume: 174 Issue: 2 Pages: 531-538

    • DOI

      10.1104/pp.17.00166

    • Related Report
      2017 Annual Research Report
    • Peer Reviewed / Open Access
  • [Journal Article] Brassinosteroid involvement in Arabidopsis thaliana stomatal opening.2017

    • Author(s)
      Inoue S, Iwashita N, Takahashi Y, Gotoh E, Okuma E, Hayashi M, Tabata R, Takemiya A, Doi M, Murata Y, Kinoshita T. and Shimazaki K.
    • Journal Title

      Plant & Cell Physiol.

      Volume: 58 Issue: 6 Pages: 1048-1058

    • DOI

      10.1093/pcp/pcx049

    • Related Report
      2016 Research-status Report
    • Peer Reviewed / Open Access / Int'l Joint Research / Acknowledgement Compliant
  • [Journal Article] A Raf-like protein kinase BHP mediates blue light-dependent stomatal opening2017

    • Author(s)
      Hayashi M, Inoue S, Ueno Y, Kinoshita T
    • Journal Title

      Scientific Reports

      Volume: 7 Issue: 1 Pages: 45586-45586

    • DOI

      10.1038/srep45586

    • Related Report
      2016 Research-status Report
    • Peer Reviewed / Open Access / Int'l Joint Research / Acknowledgement Compliant
  • [Journal Article] Auxin influx carrier AUX1 confers acid resistance for Arabidopsis root elongation through the regulation of plasma membrane H+-ATPase.2016

    • Author(s)
      Inoue S, Takahashi K, Okumura-Noda H, Kinoshita T.
    • Journal Title

      Plant & Cell Physiology

      Volume: 57 Issue: 10 Pages: 2194-2201

    • DOI

      10.1093/pcp/pcw136

    • Related Report
      2016 Research-status Report
    • Peer Reviewed / Open Access / Int'l Joint Research / Acknowledgement Compliant
  • [Journal Article] Photosynthesis activates plasma membrane H+-ATPase via sugar accumulation2016

    • Author(s)
      Okumura M, Inoue S, Kuwata K, Kinoshita T
    • Journal Title

      Plant Physiology

      Volume: 171 Pages: 580-589

    • Related Report
      2016 Research-status Report
    • Peer Reviewed / Open Access / Int'l Joint Research / Acknowledgement Compliant
  • [Journal Article] GOLDEN 2-LIKE transcription factors for chloroplast development affect ozone tolerance through the regulation of stomatal movement2016

    • Author(s)
      Yukari Nagatoshi, Nobutaka Mitsuda, Maki Hayashi, Shin-ichiro Inoue, Eiji Okuma, Akihiro Kubo, Yoshiyuki Murata, Mitsunori Seo, Hikaru Saji, Toshinori Kinoshita, and Masaru Ohme-Takagi
    • Journal Title

      PNAS

      Volume: 113 Issue: 15 Pages: 4218-4223

    • DOI

      10.1073/pnas.1513093113

    • Related Report
      2015 Research-status Report
    • Peer Reviewed
  • [Journal Article] Photosynthesis activates plasma membrane H+-ATPase via sugar accumulation in Arabidopsis leaves2016

    • Author(s)
      Masaki Okumura, Shin-ichiro Inoue, Keiko Kuwata, and Toshinori Kinoshita
    • Journal Title

      Plant Physiol

      Volume: 未確定 Issue: 1 Pages: 580-589

    • DOI

      10.1104/pp.16.00355

    • Related Report
      2015 Research-status Report
    • Peer Reviewed / Open Access
  • [Journal Article] In vitro Phosphorylation Assay of Putative Blue-Light Receptor Phototropins Using Microsomal and Plasma-membrane Fractions Prepared from Vallisneria Leaves2015

    • Author(s)
      Yuuki Sakai, Shin-ichiro Inoue, Shingo Takagi
    • Journal Title

      Bio-protocol

      Volume: 5

    • Related Report
      2015 Research-status Report
    • Peer Reviewed / Open Access
  • [Presentation] A plasma membrane syntaxin SYP132 mediates blue light-induced stomatal opening in Arabidopsis2018

    • Author(s)
      井上晋一郎、友清雄大、林真妃、奥村将樹、岡島公司、堀江智明、木下俊則、島崎研一郎
    • Organizer
      第59回 日本植物生理学会年会
    • Related Report
      2017 Annual Research Report
  • [Presentation] A plasma membrane syntaxin regulates blue light-induced stomatal opening in Arabidopsis2018

    • Author(s)
      井上晋一郎、友清雄大、林真妃、奥村将樹、岡島公司、堀江智明、木下俊則、島崎研一郎
    • Organizer
      International Symposium on Plant Photobiology
    • Related Report
      2017 Annual Research Report
    • Int'l Joint Research
  • [Presentation] A protein kinase BHP mediates blue light-dependent stomatal opening2017

    • Author(s)
      林真妃、井上晋一郎、上野宜久、木下俊則
    • Organizer
      第58回日本植物生理学会年会
    • Place of Presentation
      鹿児島大学
    • Year and Date
      2017-03-16
    • Related Report
      2016 Research-status Report
  • [Presentation] ゼニゴケにおける細胞膜H+-ATPaseの生理機能の解析2017

    • Author(s)
      中根功多朗、奥村将樹、楊為雄、井上晋一郎、石崎公庸、河内孝之、木下俊則
    • Organizer
      第58回日本植物生理学会年会
    • Place of Presentation
      鹿児島大学
    • Year and Date
      2017-03-16
    • Related Report
      2016 Research-status Report
  • [Presentation] Identification and functional analyses of the protein kinase that interacts with blue light-receptor phototropins in Arabidopsis2016

    • Author(s)
      Shin-ichiro Inoue, Eirini Kaiserli, Hirotaka Takahashi, Tatsuya Sawasaki, Toshinori Kinoshita, John M. Christie, Xiao Zhang, Atsushi Takemiya, Ken-ichiro Shimazaki
    • Organizer
      第57回日本植物生理学会年会
    • Place of Presentation
      岩手大学
    • Year and Date
      2016-03-18
    • Related Report
      2015 Research-status Report

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Published: 2015-04-16   Modified: 2019-03-29  

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