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Elucidation of the mechanism of environmental stress-specific translational regulation in plant

Research Project

Project/Area Number 15K07107
Research Category

Grant-in-Aid for Scientific Research (C)

Allocation TypeMulti-year Fund
Section一般
Research Field Plant molecular biology/Plant physiology
Research InstitutionNara Institute of Science and Technology

Principal Investigator

kato ko  奈良先端科学技術大学院大学, バイオサイエンス研究科, 准教授 (80283935)

Research Collaborator YAMASAKI Shotaro  
Project Period (FY) 2015-04-01 – 2018-03-31
Project Status Completed (Fiscal Year 2017)
Budget Amount *help
¥5,070,000 (Direct Cost: ¥3,900,000、Indirect Cost: ¥1,170,000)
Fiscal Year 2017: ¥1,300,000 (Direct Cost: ¥1,000,000、Indirect Cost: ¥300,000)
Fiscal Year 2016: ¥1,430,000 (Direct Cost: ¥1,100,000、Indirect Cost: ¥330,000)
Fiscal Year 2015: ¥2,340,000 (Direct Cost: ¥1,800,000、Indirect Cost: ¥540,000)
Keywords翻訳制御 / 環境ストレス / 植物培養細胞 / ゲノムワイド解析 / 5’UTR
Outline of Final Research Achievements

When plants are exposed to environmental stress (heat, salt etc.) the translation state changes dramatically. Translation state changes due to heat and salt stress are very similar when compared on the genome wide scale, but there are also mRNAs that differ in translation state change between two stresses such as being maintained by heat but suppressed by salt.
In this study, transient expression experiments and analysis using a transformant in which the 5'UTR of mRNA showing specific behavior was fused to a reporter gene were performed. As a result, the possibility that the CU repeat region in the 5'UTR contribute to the stress state-specific translation state change (heat suppression and salt maintenance) and the change of the translational state of mRNA under heat stress by change of transcription initiation site in response to heat stress were indicated.

Report

(4 results)
  • 2017 Annual Research Report   Final Research Report ( PDF )
  • 2016 Research-status Report
  • 2015 Research-status Report
  • Research Products

    (13 results)

All 2018 2017 2016 2015

All Journal Article (3 results) (of which Peer Reviewed: 2 results) Presentation (10 results) (of which Int'l Joint Research: 3 results,  Invited: 3 results)

  • [Journal Article] Arabidopsis thaliana cold-regulated 47 gene 5-untranslated region enables stable high-level expression of transgenes.2018

    • Author(s)
      Yamasaki, S., Sanada, Y., Imase, R., Matsuura, H., Ueno, D., Demura, T. and Kato, K.
    • Journal Title

      J. Biosci. Bioeng.

      Volume: 125 Issue: 1 Pages: 124-130

    • DOI

      10.1016/j.jbiosc.2017.08.007

    • NAID

      40021443145

    • Related Report
      2017 Annual Research Report
    • Peer Reviewed
  • [Journal Article] 植物でのタンパク質の高効率翻訳を可能にする数理モデル2017

    • Author(s)
      加藤晃、山﨑将太朗
    • Journal Title

      バイオサイエンスとインダストリー

      Volume: 75 Pages: 240-241

    • Related Report
      2017 Annual Research Report
  • [Journal Article] Compatibility of translational enhancers with various plant species2015

    • Author(s)
      Takeshi Matsui, Kazutoshi Sawada, Eiji Takita, Ko Kato
    • Journal Title

      Plant Biotechnology

      Volume: 32 Issue: 4 Pages: 309-316

    • DOI

      10.5511/plantbiotechnology.15.1103a

    • NAID

      130005115906

    • ISSN
      1342-4580, 1347-6114
    • Related Report
      2015 Research-status Report
    • Peer Reviewed
  • [Presentation] Transgene expression system optimizing translation process.2017

    • Author(s)
      加藤晃
    • Organizer
      PepTalk 2017
    • Place of Presentation
      Hilton San Diego Bayfront アメリカ、サンヂエゴ市
    • Year and Date
      2017-01-09
    • Related Report
      2016 Research-status Report
    • Int'l Joint Research / Invited
  • [Presentation] Transgene expression system in plants optimized translation process.2017

    • Author(s)
      Ko Kato
    • Organizer
      The 2nd Japan-Korea-China Trilateral Joint Symposium on Plant Biotechnology
    • Related Report
      2017 Annual Research Report
    • Int'l Joint Research / Invited
  • [Presentation] Mathematical approach to improvement of transgene expression focus on efficiency of mRNA translation.2017

    • Author(s)
      Shotaro Yamasaki, Taku Demura, Ko Kato
    • Organizer
      The 1st Asian Conference for Plant Made Pharmaceuticals
    • Related Report
      2017 Annual Research Report
    • Int'l Joint Research
  • [Presentation] 植物での有用タンパク質生産2017

    • Author(s)
      加藤晃
    • Organizer
      第5回奈良まほろば産学官連携懇話会
    • Related Report
      2017 Annual Research Report
  • [Presentation] 導入した目的遺伝子の配列に特化した翻訳エンハンサーの選抜システム2017

    • Author(s)
      山崎将太朗、上野大心、出村拓、加藤晃
    • Organizer
      第35回日本植物細胞分子生物学会
    • Related Report
      2017 Annual Research Report
  • [Presentation] 植物での転写開始点の網羅的な同定と遺伝子発現制御における転写開始点の重要性2017

    • Author(s)
      山崎将太朗、上野大心、松浦秀幸、出村拓、加藤晃
    • Organizer
      NGS現場の会、第5回研究会
    • Related Report
      2017 Annual Research Report
  • [Presentation] 高翻訳に資する5’UTRを用いた導入遺伝子高発現系2016

    • Author(s)
      今瀬諒司、山崎将太朗、出村拓、加藤晃
    • Organizer
      第34回日本植物細胞分子生物学会
    • Place of Presentation
      信州大学 長野県上田市
    • Year and Date
      2016-09-01
    • Related Report
      2016 Research-status Report
  • [Presentation] 翻訳効率を5’UTR配列から予測できる数式モデルの構築2016

    • Author(s)
      山崎将太朗、今瀬諒司、出村拓、加藤晃
    • Organizer
      第34回日本植物細胞分子生物学会
    • Place of Presentation
      信州大学 長野県上田市
    • Year and Date
      2016-09-01
    • Related Report
      2016 Research-status Report
  • [Presentation] 植物での有用遺伝子高発現系とその活用2016

    • Author(s)
      加藤晃
    • Organizer
      2016年度日本生物工学会北日本支部弘前シンポジウム
    • Place of Presentation
      弘前大学 青森県弘前市
    • Year and Date
      2016-07-23
    • Related Report
      2016 Research-status Report
    • Invited
  • [Presentation] mRNAからの翻訳効率を改良した導入遺伝子高発現系2015

    • Author(s)
      山崎将太朗、出村拓、加藤晃
    • Organizer
      第67回日本生物工学会大会
    • Place of Presentation
      鹿児島県鹿児島市
    • Year and Date
      2015-10-26
    • Related Report
      2015 Research-status Report

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Published: 2015-04-16   Modified: 2019-03-29  

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