| Budget Amount *help |
¥5,070,000 (Direct Cost: ¥3,900,000、Indirect Cost: ¥1,170,000)
Fiscal Year 2017: ¥1,430,000 (Direct Cost: ¥1,100,000、Indirect Cost: ¥330,000)
Fiscal Year 2016: ¥1,560,000 (Direct Cost: ¥1,200,000、Indirect Cost: ¥360,000)
Fiscal Year 2015: ¥2,080,000 (Direct Cost: ¥1,600,000、Indirect Cost: ¥480,000)
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| Outline of Final Research Achievements |
Developmental regulation of reproductive organ in plant is the important issue. Pollen wall development relies largely on the tapetum, which locates in anther and provides the developing microspores with metabolites, enzymes, nutrients, and structural components necessary to build up the specific wall structure called exine. Material export from the endoplasmic reticulum (ER) is mediated by coat protein complex II (COPII) vesicles. The Arabidopsis thaliana genome encodes seven homologs of SEC23, a COPII component. We analyzed knockout lines for AtSEC23A and AtSEC23D, and found atsec23ad mutant plants exhibited developmental defects in pollen and tapetal cells. These results suggest that AtSEC23A and AtSEC23D may organize pollen wall development and exine patterning by regulating ER export of lipids and proteins necessary for pollen wall formation.
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