Function of vesicle transport system for development of plant reproductive organs
| Project/Area Number |
15K07109
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| Research Category |
Grant-in-Aid for Scientific Research (C)
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| Allocation Type | Multi-year Fund |
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| Section | 一般 |
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| Research Field |
Plant molecular biology/Plant physiology
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| Research Institution | Shimane University |
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Principal Investigator |
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| Project Period (FY) |
2015-04-01 – 2018-03-31
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| Project Status |
Completed (Fiscal Year 2017)
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| Budget Amount *help |
¥5,070,000 (Direct Cost: ¥3,900,000、Indirect Cost: ¥1,170,000)
Fiscal Year 2017: ¥1,430,000 (Direct Cost: ¥1,100,000、Indirect Cost: ¥330,000)
Fiscal Year 2016: ¥1,560,000 (Direct Cost: ¥1,200,000、Indirect Cost: ¥360,000)
Fiscal Year 2015: ¥2,080,000 (Direct Cost: ¥1,600,000、Indirect Cost: ¥480,000)
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| Keywords | 花粉 / タペート細胞 / 小胞輸送 / シロイヌナズナ / 小胞輸送因子 / 生殖器官 |
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| Outline of Final Research Achievements |
Developmental regulation of reproductive organ in plant is the important issue. Pollen wall development relies largely on the tapetum, which locates in anther and provides the developing microspores with metabolites, enzymes, nutrients, and structural components necessary to build up the specific wall structure called exine. Material export from the endoplasmic reticulum (ER) is mediated by coat protein complex II (COPII) vesicles. The Arabidopsis thaliana genome encodes seven homologs of SEC23, a COPII component. We analyzed knockout lines for AtSEC23A and AtSEC23D, and found atsec23ad mutant plants exhibited developmental defects in pollen and tapetal cells. These results suggest that AtSEC23A and AtSEC23D may organize pollen wall development and exine patterning by regulating ER export of lipids and proteins necessary for pollen wall formation.
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Report
(4 results)
Research Products
(48 results)
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[Journal Article] Development of an R4 dual-site (R4DS) gateway cloning system enabling the efficient simultaneous cloning of two desired sets of promoters and open reading frames in a binary vector for plant research.2017
Author(s)
Aboulela, M., Tanaka, Y., Nishimura, K., Mano, S., Nishimura, M., Ishiguro, S., Kimura, T., and Nakagawa, T.
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Journal Title
PLoS One
Volume: 12
Issue: 5
Pages: 177889-177901
DOI
Related Report
Peer Reviewed / Open Access
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[Journal Article] A dual-site gateway cloning system for simultaneous cloning of two genes for plant transformation.2017
Author(s)
Aboulela, M., Tanaka, Y., Nishimura, K., Mano, S., Kimura, T., and Nakagawa, T.
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Journal Title
Plasmid
Volume: 92
Pages: 1-11
DOI
Related Report
Peer Reviewed
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[Journal Article] The tyrosine-sorting motif of the vacuolar sorting receptor VSR4 from Arabidopsis thaliana, which is involved in the interaction between VSR4 and AP1M2, mu1-adaptin type 2 of clathrin adaptor complex 1 subunits, participates in the post-Golgi sorting of VSR42016
Author(s)
Nishimura K., Matsunami E., Yoshida S., Kohata S., Yamauchi J., Jisaka M., Nagaya T., Yokota K. and Nakagawa T.
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Journal Title
Bioscience, Biotechnology, and Biochemistry
Volume: 80
Issue: 4
Pages: 694-705
DOI
Related Report
Peer Reviewed / Int'l Joint Research / Acknowledgement Compliant
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[Journal Article] New Gateway-compatible vectors for high-throughput protein-protein interaction analysis by a bimolecular fluorescence complementation (BiFC) assay in plants and their application to a plant claghrin structure analysis2015
Author(s)
Nishimura K., Ishikawa S., Matsunami E., Yamauchi J., Homma K., Faulkner C., Oparka K., Jisaka M., Nagaya T., Yokota K. and Nakagawa T.
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Journal Title
Bioscience, Biotechnology, and Biochemistry
Volume: 79
Issue: 12
Pages: 1995-2006
DOI
Related Report
Peer Reviewed / Int'l Joint Research / Acknowledgement Compliant
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