| Budget Amount *help |
¥5,200,000 (Direct Cost: ¥4,000,000、Indirect Cost: ¥1,200,000)
Fiscal Year 2017: ¥1,430,000 (Direct Cost: ¥1,100,000、Indirect Cost: ¥330,000)
Fiscal Year 2016: ¥1,950,000 (Direct Cost: ¥1,500,000、Indirect Cost: ¥450,000)
Fiscal Year 2015: ¥1,820,000 (Direct Cost: ¥1,400,000、Indirect Cost: ¥420,000)
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| Outline of Final Research Achievements |
In oxygenic photoynthesis, there are several hydrogen bond networks between Mn cluster and lumen. To study role of these hydrogen bond networks, we have transformed Chlamydomonas chloroplast to substitute the participating amino acid residues of chloroplast-encoded PSII subunits with 19 other amino acids. We have examined their photosynthetic growth, PSII accumulation and activity. Analysis of the resulting transformants, we found that one of the hydrogen bond networks including D1-D61, D1-E65 and D2-E312 could be involved in excretion of protons generated during water oxidation at the Mn cluster. One of the hydrogen bond networks starting from YZ does not seem to be proton exit pathway.
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