Assemblage process of photosystem II complex

Research Project

Project/Area Number	15K07112
Research Category	Grant-in-Aid for Scientific Research (C)
Allocation Type	Multi-year Fund
Section	一般
Research Field	Plant molecular biology/Plant physiology
Research Institution	University of Hyogo
Principal Investigator	Kashino Yasuhiro 兵庫県立大学, 生命理学研究科, 准教授 (20221872)
Project Period (FY)	2015-04-01 – 2018-03-31
Project Status	Completed (Fiscal Year 2017)
Budget Amount *help	¥5,200,000 (Direct Cost: ¥4,000,000、Indirect Cost: ¥1,200,000) Fiscal Year 2017: ¥1,560,000 (Direct Cost: ¥1,200,000、Indirect Cost: ¥360,000) Fiscal Year 2016: ¥1,560,000 (Direct Cost: ¥1,200,000、Indirect Cost: ¥360,000) Fiscal Year 2015: ¥2,080,000 (Direct Cost: ¥1,600,000、Indirect Cost: ¥480,000)
Keywords	光化学系II / タンパク質複合体の構築 / クロロフィル合成 / シアノバクテリア / 熱安定性 / 光化学系II複合体 / 膜タンパク質複合体
Outline of Final Research Achievements	Photosystem II (PSII) is a multi-protein complex containing ~20 membrane proteins that converts water to oxygen. Assembling process of PSII was investigated in this work using a uniquely developed strain that starts accumulation of PSII upon light illumination. Among several proteins that were found to be responsible for only assembling process, three proteins were especially important for the stable accumulation of functional PSII. Further, it was revealed that the assemblage machinery was very flexible such that inactivation of individual gene responsible for the assemblage of PSII was complemented by some unknown mechanism.