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Quiescent center-specific regulation of ribosome levels and elucidation of its developmental function

Research Project

Project/Area Number 15K07115
Research Category

Grant-in-Aid for Scientific Research (C)

Allocation TypeMulti-year Fund
Section一般
Research Field Plant molecular biology/Plant physiology
Research InstitutionRikkyo University

Principal Investigator

HORIGUCHI Gorou  立教大学, 理学部, 准教授 (70342847)

Project Period (FY) 2015-04-01 – 2018-03-31
Project Status Completed (Fiscal Year 2017)
Budget Amount *help
¥5,200,000 (Direct Cost: ¥4,000,000、Indirect Cost: ¥1,200,000)
Fiscal Year 2017: ¥2,080,000 (Direct Cost: ¥1,600,000、Indirect Cost: ¥480,000)
Fiscal Year 2016: ¥1,690,000 (Direct Cost: ¥1,300,000、Indirect Cost: ¥390,000)
Fiscal Year 2015: ¥1,430,000 (Direct Cost: ¥1,100,000、Indirect Cost: ¥330,000)
Keywordsシロイヌナズナ / 根端分裂組織 / 静止中心 / リボソームタンパク質 / RPL4D / ブラシノステロイド / リボソーム / サイトカイニン / 細胞周期
Outline of Final Research Achievements

Quiescent center (QC) resides in the center of a root apical meristem and has a very low mitotic activity. To establish this quiescence, the amount of ribosomes is assumed to be maintained at a low level. In this study, overexpression of ribosomal protein fused with GFP revealed that at least several distinct ribosomal proteins are maintained at a low level in the QC cells. This regulation is counteracted by the exogenous application of brassinosteroids or cytokinins. In addition, such quantitative regulation of ribosomal proteins requires two mechanisms: one of them suppresses ribosome production in the QC cells and the other eliminates ribosomal proteins produced in excess in entire root apical meristem.

Report

(4 results)
  • 2017 Annual Research Report   Final Research Report ( PDF )
  • 2016 Research-status Report
  • 2015 Research-status Report
  • Research Products

    (20 results)

All 2018 2017 2016 2015 Other

All Journal Article (4 results) (of which Int'l Joint Research: 1 results,  Peer Reviewed: 4 results,  Open Access: 3 results) Presentation (15 results) (of which Int'l Joint Research: 1 results,  Invited: 3 results) Remarks (1 results)

  • [Journal Article] Plastid gene expression is essential for lateral root stem-cell patterning, symplastic connectivity, and root plastid distribution in Arabidopsis thaliana2018

    • Author(s)
      Nakata MT, Sato M, Wakazaki M, Sato N, Kojima K Sekine A, Nakamura S, Shikanai T, Toyooka K, Tsukaya H, Horiguchi G
    • Journal Title

      Biology Open

      Volume: 7 Pages: 028175-028175

    • DOI

      10.1242/bio.028175

    • Related Report
      2017 Annual Research Report
    • Peer Reviewed / Open Access
  • [Journal Article] Two nucleolar proteins, GDP1 and OLI2, function as ribosome biogenesis factors and are preferentially involved in promotion of leaf cell proliferation without strongly affecting leaf adaxial-abaxial patterning in Arabidopsis thaliana.2018

    • Author(s)
      Kojima K, Tamura J, Chiba H, Fukuda K, Tsukaya H, Horiguchi G
    • Journal Title

      Frontiers in Plant Science

      Volume: ー Pages: 2240-2240

    • DOI

      10.3389/fpls.2017.02240

    • Related Report
      2017 Annual Research Report
    • Peer Reviewed / Open Access
  • [Journal Article] Evidence for a role of ANAC082 as a ribosomal stress response mediator leading to growth defects and developmental alterations in Arabidopsis2017

    • Author(s)
      Iwai Ohbayashi, Chung-Yi Lin, Naoki Shinohara, Yoko Matsumura, Yasunori Machida, Gorou Horiguchi, Hirokazu Tsukaya, Munetaka Sugiyama
    • Journal Title

      The Plant Cell

      Volume: 29 Issue: 10 Pages: 2644-2660

    • DOI

      10.1105/tpc.17.00255

    • Related Report
      2017 Annual Research Report
    • Peer Reviewed / Open Access / Int'l Joint Research
  • [Journal Article] リボソームの生合成と植物の発生2017

    • Author(s)
      堀口 吾朗
    • Journal Title

      生物科学

      Volume: 印刷中

    • Related Report
      2016 Research-status Report
    • Peer Reviewed
  • [Presentation] A Critical Role of the NAC transcription Factor ANAC082 in Ribosomal Stress Signaling of Plant Cells2018

    • Author(s)
      Iwai Ohbayashi, Shun Sasaki, Chung-Yi Lin, Naoki Shinohara, Yoko Matsumura, Yasunori Machida, Gorou Horiguchi, Hirokazu Tsukaya, Masahiko Furutani, Hitoshi Onouchi, and Munetaka Sugiyama
    • Organizer
      第59回日本植物生理学会年会
    • Related Report
      2017 Annual Research Report
    • Invited
  • [Presentation] A quartet of NAC transcription factor genes is upregulated in response to abnormal ribosomal proteins and enhances leaf abaxialization in asymmetric leaves22018

    • Author(s)
      Gorou Horiguchi, Iwai Ohbayashi, Munetaka Sugiyama, and Hirokazu Tsukaya
    • Organizer
      第59回日本植物生理学会年会
    • Related Report
      2017 Annual Research Report
    • Invited
  • [Presentation] Four NAC transcription factor genes are involved in rpl4d-induced leaf abaxialization in the as2 mutant of Arabidopsis thaliana2017

    • Author(s)
      Gorou Horiguchi, Iwai Ohbayashi, Munetaka Sugiyama, Hirokazu Tsukaya
    • Organizer
      第58回日本植物生理学会年会
    • Place of Presentation
      鹿児島県鹿児島市鹿児島大学
    • Year and Date
      2017-03-16
    • Related Report
      2016 Research-status Report
  • [Presentation] Transfer RNA wobble U34 modification in plants: Its possible relation to leaf development2017

    • Author(s)
      Yumi Nakai, Gorou Horiguchi, Akiko Harada, Masato Nakai, Takato Yano
    • Organizer
      第58回日本植物生理学会年会
    • Place of Presentation
      鹿児島県鹿児島市鹿児島大学
    • Year and Date
      2017-03-16
    • Related Report
      2016 Research-status Report
  • [Presentation] How mutations in ribosome-related genes affect leaf adaxial-abaxial patterning in Arabidopsis thaliana2017

    • Author(s)
      Gorou Horiguchi, Hirokazu Tsukaya
    • Organizer
      50th Annual Meeting of the Japanese Society of Developmental Biologists
    • Related Report
      2017 Annual Research Report
    • Invited
  • [Presentation] tRNAwobbleU34修飾欠損のシロイヌナズナ葉の形態形成への影響2017

    • Author(s)
      中井 由実、堀口 吾朗、岩渕 功誠、田村 謙太郎、原田 明子、中井 正人、西村 いくこ、矢野 貴人
    • Organizer
      2017年度生命科学系学会合同年次大会
    • Related Report
      2017 Annual Research Report
  • [Presentation] シロイヌナズナas2 rpl4dにおける葉の背軸化を抑圧するリボソームタンパク質 変異の解析2017

    • Author(s)
      深田 かなえ、高原 正裕、塚谷 裕一、堀口 吾朗
    • Organizer
      日本植物学会第81回大会
    • Related Report
      2017 Annual Research Report
  • [Presentation] シロイヌナズナのリボソームストレス応答とNAC転写因子ANAC082の役割2017

    • Author(s)
      大林 祝、林 忠逸、篠原 直貴、松村 葉子、町田 泰則、堀口 吾朗、塚谷 裕一、杉山 宗隆
    • Organizer
      日本植物学会第81回大会
    • Related Report
      2017 Annual Research Report
  • [Presentation] 植物tRNAのwobble位修飾とロゼッタ葉の形態形成について2016

    • Author(s)
      中井 由実、堀口 吾朗、原田 朋子、中井 正人、矢野 貴人
    • Organizer
      第39回日本分子生物学会年会
    • Place of Presentation
      神奈川県横浜市パシフィコ横浜
    • Year and Date
      2016-11-30
    • Related Report
      2016 Research-status Report
  • [Presentation] リボソーム生合成関連因子GDP1とOLI2が葉の発生に果たす役割の解析2016

    • Author(s)
      深田 かなえ、塚谷 裕一、堀口 吾朗
    • Organizer
      日本植物学会第80回大会
    • Place of Presentation
      沖縄県宜野湾市沖縄コンベンションセンター
    • Year and Date
      2016-09-16
    • Related Report
      2016 Research-status Report
  • [Presentation] Mutations in ribosomal protein genes induce the expression of NAC transcription factor gene, SUZAKU1, and promote leaf abaxialization in asymmetric leaves22016

    • Author(s)
      Gorou Horiguchi, Mikito Inoue, Hidenori Masuda, Miyuki Nakata, and Hirokazu Tsukaya
    • Organizer
      The 27th International Conference on Arabidopsis Research
    • Place of Presentation
      Gyeong Ju, Korea
    • Year and Date
      2016-06-29
    • Related Report
      2016 Research-status Report
    • Int'l Joint Research
  • [Presentation] Leaf abaxialization in rpl4 as2 mutants requires aberrant rpl4 transcript accumulation, a RING finger protein gene SZK2, and upregulation of a NAC transcription factor gene SZK12016

    • Author(s)
      Gorou Horiugchi, Mikito Inoue, Hidenori Masuda, Masahiro Takahara, Miyuki Nakata, Hirokazu Tsukaya
    • Organizer
      第57回日本植物生理学会年会
    • Place of Presentation
      岩手県盛岡市岩手大学
    • Year and Date
      2016-03-18
    • Related Report
      2015 Research-status Report
  • [Presentation] as2 rpl4dで引き起こされる葉の背軸化に必要なSZK1の発現解析2015

    • Author(s)
      井上幹人、中田未友希、塚谷裕一、堀口吾朗
    • Organizer
      日本植物学会第79回大会
    • Place of Presentation
      新潟県新潟市朱鷺メッセ
    • Year and Date
      2015-09-06
    • Related Report
      2015 Research-status Report
  • [Presentation] 異なるrpl4dおよびrpl4aアリルで蓄積している変異型mRNAの構造決定とその背腹性制御に及ぼす影響の解析2015

    • Author(s)
      増田秀典、塚谷裕一、堀口吾朗
    • Organizer
      日本植物学会第79回大会
    • Place of Presentation
      新潟県新潟市朱鷺メッセ
    • Year and Date
      2015-09-06
    • Related Report
      2015 Research-status Report
  • [Presentation] SUZAKU1, a NAC-domain transcription factor gene promotes leaf abaxialization in response to as2-enhancer mutations in Arabidopsis thaliana2015

    • Author(s)
      Gorou Horiguchi, Mikito Inoue, Masuda Hidenori, Miyuki Nakata, Hirokazu Tsukaya
    • Organizer
      第48回日本発生生物学会大会
    • Place of Presentation
      茨城県つくば市エポカルつくば
    • Year and Date
      2015-06-02
    • Related Report
      2015 Research-status Report
  • [Remarks] 立教大学理学部生命理学科植物分子発生学研究室ホームページ

    • URL

      http://www2.rikkyo.ac.jp/web/horiguchi/plant/home.html

    • Related Report
      2017 Annual Research Report 2016 Research-status Report

URL: 

Published: 2015-04-16   Modified: 2019-03-29  

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