Quiescent center-specific regulation of ribosome levels and elucidation of its developmental function

• Project/Area Number: 15K07115
• Research Category: Grant-in-Aid for Scientific Research (C)
• Allocation Type: Multi-year Fund
• Section: 一般
• Research Field: Plant molecular biology/Plant physiology
• Research Institution: Rikkyo University
• Principal Investigator: HORIGUCHI Gorou 立教大学, 理学部, 准教授 (70342847)
• Project Period (FY): 2015-04-01 – 2018-03-31
• Project Status: Completed (Fiscal Year 2017)
• Budget Amount: ¥5,200,000 (Direct Cost: ¥4,000,000、Indirect Cost: ¥1,200,000); Fiscal Year 2017: ¥2,080,000 (Direct Cost: ¥1,600,000、Indirect Cost: ¥480,000); Fiscal Year 2016: ¥1,690,000 (Direct Cost: ¥1,300,000、Indirect Cost: ¥390,000); Fiscal Year 2015: ¥1,430,000 (Direct Cost: ¥1,100,000、Indirect Cost: ¥330,000)
• Keywords: シロイヌナズナ / 根端分裂組織 / 静止中心 / リボソームタンパク質 / RPL4D / ブラシノステロイド / リボソーム / サイトカイニン / 細胞周期

Outline of Final Research Achievements

Quiescent center (QC) resides in the center of a root apical meristem and has a very low mitotic activity. To establish this quiescence, the amount of ribosomes is assumed to be maintained at a low level. In this study, overexpression of ribosomal protein fused with GFP revealed that at least several distinct ribosomal proteins are maintained at a low level in the QC cells. This regulation is counteracted by the exogenous application of brassinosteroids or cytokinins. In addition, such quantitative regulation of ribosomal proteins requires two mechanisms: one of them suppresses ribosome production in the QC cells and the other eliminates ribosomal proteins produced in excess in entire root apical meristem.

Research Products

• [Journal Article] Plastid gene expression is essential for lateral root stem-cell patterning, symplastic connectivity, and root plastid distribution in Arabidopsis thaliana (2018)
  • Author(s): Nakata MT, Sato M, Wakazaki M, Sato N, Kojima K Sekine A, Nakamura S, Shikanai T, Toyooka K, Tsukaya H, Horiguchi G
  • Journal Title: Biology Open Volume: 7 Pages: 028175-028175
  • DOI: 10.1242/bio.028175
  • Peer Reviewed / Open Access
• [Journal Article] Two nucleolar proteins, GDP1 and OLI2, function as ribosome biogenesis factors and are preferentially involved in promotion of leaf cell proliferation without strongly affecting leaf adaxial-abaxial patterning in Arabidopsis thaliana. (2018)
  • Author(s): Kojima K, Tamura J, Chiba H, Fukuda K, Tsukaya H, Horiguchi G
  • Journal Title: Frontiers in Plant Science Volume: ー Pages: 2240-2240
  • DOI: 10.3389/fpls.2017.02240
  • Peer Reviewed / Open Access
• [Journal Article] Evidence for a role of ANAC082 as a ribosomal stress response mediator leading to growth defects and developmental alterations in Arabidopsis (2017)
  • Author(s): Iwai Ohbayashi, Chung-Yi Lin, Naoki Shinohara, Yoko Matsumura, Yasunori Machida, Gorou Horiguchi, Hirokazu Tsukaya, Munetaka Sugiyama
  • Journal Title: The Plant Cell Volume: 29 Issue: 10 Pages: 2644-2660
  • DOI: 10.1105/tpc.17.00255
  • Peer Reviewed / Open Access / Int'l Joint Research
• [Journal Article] リボソームの生合成と植物の発生 (2017)
  • Author(s): 堀口 吾朗
  • Journal Title: 生物科学 Volume: 印刷中
  • Peer Reviewed
• [Presentation] A Critical Role of the NAC transcription Factor ANAC082 in Ribosomal Stress Signaling of Plant Cells (2018)
  • Author(s): Iwai Ohbayashi, Shun Sasaki, Chung-Yi Lin, Naoki Shinohara, Yoko Matsumura, Yasunori Machida, Gorou Horiguchi, Hirokazu Tsukaya, Masahiko Furutani, Hitoshi Onouchi, and Munetaka Sugiyama
  • Organizer: 第59回日本植物生理学会年会
  • Invited
• [Presentation] A quartet of NAC transcription factor genes is upregulated in response to abnormal ribosomal proteins and enhances leaf abaxialization in asymmetric leaves2 (2018)
  • Author(s): Gorou Horiguchi, Iwai Ohbayashi, Munetaka Sugiyama, and Hirokazu Tsukaya
  • Organizer: 第59回日本植物生理学会年会
  • Invited
• [Presentation] Four NAC transcription factor genes are involved in rpl4d-induced leaf abaxialization in the as2 mutant of Arabidopsis thaliana (2017)
  • Author(s): Gorou Horiguchi, Iwai Ohbayashi, Munetaka Sugiyama, Hirokazu Tsukaya
  • Organizer: 第58回日本植物生理学会年会
  • Place of Presentation: 鹿児島県鹿児島市鹿児島大学
  • Year and Date: 2017-03-16
• [Presentation] Transfer RNA wobble U34 modification in plants: Its possible relation to leaf development (2017)
  • Author(s): Yumi Nakai, Gorou Horiguchi, Akiko Harada, Masato Nakai, Takato Yano
  • Organizer: 第58回日本植物生理学会年会
  • Place of Presentation: 鹿児島県鹿児島市鹿児島大学
  • Year and Date: 2017-03-16
• [Presentation] How mutations in ribosome-related genes affect leaf adaxial-abaxial patterning in Arabidopsis thaliana (2017)
  • Author(s): Gorou Horiguchi, Hirokazu Tsukaya
  • Organizer: 50th Annual Meeting of the Japanese Society of Developmental Biologists
  • Invited
• [Presentation] tRNAwobbleU34修飾欠損のシロイヌナズナ葉の形態形成への影響 (2017)
  • Author(s): 中井 由実、堀口 吾朗、岩渕 功誠、田村 謙太郎、原田 明子、中井 正人、西村 いくこ、矢野 貴人
  • Organizer: 2017年度生命科学系学会合同年次大会
• [Presentation] シロイヌナズナas2 rpl4dにおける葉の背軸化を抑圧するリボソームタンパク質 変異の解析 (2017)
  • Author(s): 深田 かなえ、高原 正裕、塚谷 裕一、堀口 吾朗
  • Organizer: 日本植物学会第81回大会
• [Presentation] シロイヌナズナのリボソームストレス応答とNAC転写因子ANAC082の役割 (2017)
  • Author(s): 大林 祝、林 忠逸、篠原 直貴、松村 葉子、町田 泰則、堀口 吾朗、塚谷 裕一、杉山 宗隆
  • Organizer: 日本植物学会第81回大会
• [Presentation] 植物tRNAのwobble位修飾とロゼッタ葉の形態形成について (2016)
  • Author(s): 中井 由実、堀口 吾朗、原田 朋子、中井 正人、矢野 貴人
  • Organizer: 第39回日本分子生物学会年会
  • Place of Presentation: 神奈川県横浜市パシフィコ横浜
  • Year and Date: 2016-11-30
• [Presentation] リボソーム生合成関連因子GDP1とOLI2が葉の発生に果たす役割の解析 (2016)
  • Author(s): 深田 かなえ、塚谷 裕一、堀口 吾朗
  • Organizer: 日本植物学会第80回大会
  • Place of Presentation: 沖縄県宜野湾市沖縄コンベンションセンター
  • Year and Date: 2016-09-16
• [Presentation] Mutations in ribosomal protein genes induce the expression of NAC transcription factor gene, SUZAKU1, and promote leaf abaxialization in asymmetric leaves2 (2016)
  • Author(s): Gorou Horiguchi, Mikito Inoue, Hidenori Masuda, Miyuki Nakata, and Hirokazu Tsukaya
  • Organizer: The 27th International Conference on Arabidopsis Research
  • Place of Presentation: Gyeong Ju, Korea
  • Year and Date: 2016-06-29
  • Int'l Joint Research
• [Presentation] Leaf abaxialization in rpl4 as2 mutants requires aberrant rpl4 transcript accumulation, a RING finger protein gene SZK2, and upregulation of a NAC transcription factor gene SZK1 (2016)
  • Author(s): Gorou Horiugchi, Mikito Inoue, Hidenori Masuda, Masahiro Takahara, Miyuki Nakata, Hirokazu Tsukaya
  • Organizer: 第57回日本植物生理学会年会
  • Place of Presentation: 岩手県盛岡市岩手大学
  • Year and Date: 2016-03-18
• [Presentation] as2 rpl4dで引き起こされる葉の背軸化に必要なSZK1の発現解析 (2015)
  • Author(s): 井上幹人、中田未友希、塚谷裕一、堀口吾朗
  • Organizer: 日本植物学会第79回大会
  • Place of Presentation: 新潟県新潟市朱鷺メッセ
  • Year and Date: 2015-09-06
• [Presentation] 異なるrpl4dおよびrpl4aアリルで蓄積している変異型mRNAの構造決定とその背腹性制御に及ぼす影響の解析 (2015)
  • Author(s): 増田秀典、塚谷裕一、堀口吾朗
  • Organizer: 日本植物学会第79回大会
  • Place of Presentation: 新潟県新潟市朱鷺メッセ
  • Year and Date: 2015-09-06
• [Presentation] SUZAKU1, a NAC-domain transcription factor gene promotes leaf abaxialization in response to as2-enhancer mutations in Arabidopsis thaliana (2015)
  • Author(s): Gorou Horiguchi, Mikito Inoue, Masuda Hidenori, Miyuki Nakata, Hirokazu Tsukaya
  • Organizer: 第48回日本発生生物学会大会
  • Place of Presentation: 茨城県つくば市エポカルつくば
  • Year and Date: 2015-06-02
• [Remarks] 立教大学理学部生命理学科植物分子発生学研究室ホームページ
  • URL: http://www2.rikkyo.ac.jp/web/horiguchi/plant/home.html