Mechanisms of epigenetic regulation in establishment of leaf polarity in plants
Project/Area Number |
15K07116
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Research Category |
Grant-in-Aid for Scientific Research (C)
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Allocation Type | Multi-year Fund |
Section | 一般 |
Research Field |
Plant molecular biology/Plant physiology
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Research Institution | Chubu University |
Principal Investigator |
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Co-Investigator(Kenkyū-buntansha) |
高橋 広夫 金沢大学, 薬学系, 准教授 (30454367)
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Co-Investigator(Renkei-kenkyūsha) |
KOJIMA Shoko 中部大学, 応用生物学部, 准教授 (10340209)
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Project Period (FY) |
2015-04-01 – 2018-03-31
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Project Status |
Completed (Fiscal Year 2017)
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Budget Amount *help |
¥4,940,000 (Direct Cost: ¥3,800,000、Indirect Cost: ¥1,140,000)
Fiscal Year 2017: ¥1,170,000 (Direct Cost: ¥900,000、Indirect Cost: ¥270,000)
Fiscal Year 2016: ¥1,170,000 (Direct Cost: ¥900,000、Indirect Cost: ¥270,000)
Fiscal Year 2015: ¥2,600,000 (Direct Cost: ¥2,000,000、Indirect Cost: ¥600,000)
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Keywords | 発生・分化 / エピジェネティクス / シロイヌナズナ / 葉器官形成 / DNAメチル化 / gene body methylation / 遺伝子発現制御 / 葉の発生分化 / gene body メチル化 / 核小体 / クロマチン構造 |
Outline of Final Research Achievements |
Arabidopsis AS2 plays a critical role in leaf adaxial-abaxial partitioning by repressing expression of the abaxial-determining gene ETT/ARF3. We previously reported that six CpG in its exon 6 are highly methylated by MET1, that CpG methylation levels are inversely correlated with ETT/ARF3 transcript levels. Methylation levels are decreased in as2. All these imply that AS2 is involved in epigenetic repression of ETT/ARF3 by gene body DNA methylation. The mechanism of the epigenetic repression by AS2, however, is unknown. We tested mutations of NUC1 and RH10 encoding nucleolus-localized proteins for the methylation as these mutations enhance the level of ETT/ARF3 transcripts in as2-1. Methylation levels were decreased in rh10 and nuc1, implying that RH10, NUC1 are involved in maintaining methylation. Furthermore, we found that AS2 binds specifically the sequence containing CpGs in exon 1 of ETT/ARF3, and that the binding requires the zinc-finger-like motif in AS2.
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Report
(4 results)
Research Products
(54 results)
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[Journal Article] A genetic link between epigenetic repressor AS1-AS2 and a putative small subunit processome in leaf polarity establishment of Arabidopsis2016
Author(s)
Matsumura Y., Ohbayashi I., Takahashi H., Kojima S., Ishibashi N., Keta S., Nakagawa A., Hayashi R., Saez-Vasquez J., Echeverria M., Sugiyama M., Nakamura K., Machida C. and Machida Y.
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Journal Title
Biol. Open
Volume: 5
Issue: 7
Pages: 942-954
DOI
Related Report
Peer Reviewed / Open Access / Int'l Joint Research / Acknowledgement Compliant
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[Presentation] The nucleolus and plant-specific nuclear protein complex AS1-AS2 play a critical role in establishment of the dorso-ventral polarity of Arabidopsis leaves.2016
Author(s)
Machida Y., Matsumura Y., Ohbayashi I., Takahashi H., Kojima S., Nakagawa A., Keta S., Hayashi R., Sugiyama M., Nakamura K., Machida C.
Organizer
The 39th Annual Meeting of the Molecular Biology Sciety of Japan. In symposium of Cell fate regulation orchestrated by ribonucleoprotein macromolecular complex.
Place of Presentation
Yokohama City, Pacifico Yokohama
Year and Date
2016-11-30
Related Report
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[Presentation] The nucleolus and plant-specific nuclear protein complex AS1-AS2 play a critical role in establishment of the dorso-ventral polarity of Arabidopsis leaves.2016
Author(s)
Machida Y., Matsumura Y., Ohbayashi I., Takahashi H., Kojima S., Nakagawa A., Keta S., Hayashi R., Sugiyama M., Nakamura K., Machida C.
Organizer
The 39th Annual Meeting of the Molecular Biology Sciety of Japan
Place of Presentation
Yokohama City, Pacifico Yokohama
Year and Date
2016-11-30
Related Report
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