| Project/Area Number |
15K07116
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| Research Category |
Grant-in-Aid for Scientific Research (C)
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| Allocation Type | Multi-year Fund |
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| Section | 一般 |
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| Research Field |
Plant molecular biology/Plant physiology
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| Research Institution | Chubu University |
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Principal Investigator |
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| Co-Investigator(Kenkyū-buntansha) |
高橋 広夫 金沢大学, 薬学系, 准教授 (30454367)
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| Co-Investigator(Renkei-kenkyūsha) |
KOJIMA Shoko 中部大学, 応用生物学部, 准教授 (10340209)
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| Project Period (FY) |
2015-04-01 – 2018-03-31
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| Project Status |
Completed (Fiscal Year 2017)
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| Budget Amount *help |
¥4,940,000 (Direct Cost: ¥3,800,000、Indirect Cost: ¥1,140,000)
Fiscal Year 2017: ¥1,170,000 (Direct Cost: ¥900,000、Indirect Cost: ¥270,000)
Fiscal Year 2016: ¥1,170,000 (Direct Cost: ¥900,000、Indirect Cost: ¥270,000)
Fiscal Year 2015: ¥2,600,000 (Direct Cost: ¥2,000,000、Indirect Cost: ¥600,000)
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| Keywords | 発生・分化 / エピジェネティクス / シロイヌナズナ / 葉器官形成 / DNAメチル化 / gene body methylation / 遺伝子発現制御 / 葉の発生分化 / gene body メチル化 / 核小体 / クロマチン構造 |
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| Outline of Final Research Achievements |
Arabidopsis AS2 plays a critical role in leaf adaxial-abaxial partitioning by repressing expression of the abaxial-determining gene ETT/ARF3. We previously reported that six CpG in its exon 6 are highly methylated by MET1, that CpG methylation levels are inversely correlated with ETT/ARF3 transcript levels. Methylation levels are decreased in as2. All these imply that AS2 is involved in epigenetic repression of ETT/ARF3 by gene body DNA methylation. The mechanism of the epigenetic repression by AS2, however, is unknown. We tested mutations of NUC1 and RH10 encoding nucleolus-localized proteins for the methylation as these mutations enhance the level of ETT/ARF3 transcripts in as2-1. Methylation levels were decreased in rh10 and nuc1, implying that RH10, NUC1 are involved in maintaining methylation. Furthermore, we found that AS2 binds specifically the sequence containing CpGs in exon 1 of ETT/ARF3, and that the binding requires the zinc-finger-like motif in AS2.
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