Study on seed germination regulated by peroxisomal membrane ABC transporter

• Project/Area Number: 15K07117
• Research Category: Grant-in-Aid for Scientific Research (C)
• Allocation Type: Multi-year Fund
• Section: 一般
• Research Field: Plant molecular biology/Plant physiology
• Research Institution: Nagahama Institute of Bio-Science and Technology
• Principal Investigator: Hayashi Makoto 長浜バイオ大学, バイオサイエンス学部, 教授 (50212155)
• Project Period (FY): 2015-04-01 – 2018-03-31
• Project Status: Completed (Fiscal Year 2017)
• Budget Amount: ¥4,940,000 (Direct Cost: ¥3,800,000、Indirect Cost: ¥1,140,000); Fiscal Year 2017: ¥1,950,000 (Direct Cost: ¥1,500,000、Indirect Cost: ¥450,000); Fiscal Year 2016: ¥1,560,000 (Direct Cost: ¥1,200,000、Indirect Cost: ¥360,000); Fiscal Year 2015: ¥1,430,000 (Direct Cost: ¥1,100,000、Indirect Cost: ¥330,000)
• Keywords: ペルオキシソーム / 種子発芽 / 貯蔵脂肪 / デンプン / 葉酸 / プラスチド / 発芽 / 脂質代謝 / 糖代謝 / グリオキシソーム / アブシジン酸 / ムシラゲ / 脂質分解

Outline of Final Research Achievements

PED3 is a peroxisomal membrane ABC transporter. Fatty acids released from seed reserved lipid in oil body are imported into peroxisome by PED3, and then converted into sucrose supplying essential energy for seed germination.
To identify genes regulating carbon metabolism during germination, we performed comprehensive isolation and molecular genetical analyses of ped3 suppressor mutants. Six mutants were so far identified and analyzed their phenotypes. One of the mutant was revealed that it had defect in plastidial folylpolyglutamate synthetase gene, and accumulated starch during germination in the dark.

Research Products

• [Journal Article] Plastidial Folate Prevents Starch Biosynthesis Triggered by Sugar Influx into Non-Photosynthetic Plastids of Arabidopsis (2017)
  • Author(s): Hayashi Makoto、Tanaka Mina、Yamamoto Saki、Nakagawa Taro、Kanai Masatake、Anegawa Aya、Ohnishi Miwa、Mimura Tetsuro、Nishimura Mikio
  • Journal Title: Plant and Cell Physiology Volume: 58 Issue: 8 Pages: 1328-1338
  • DOI: 10.1093/pcp/pcx076
  • NAID: 120006473630
  • Peer Reviewed / Open Access
• [Journal Article] Membrane Dynamics and Multiple Functions of Oil Bodies in Seeds and Leaves (2017)
  • Author(s): Shimada Takashi L.、Hayashi Makoto、Hara-Nishimura Ikuko
  • Journal Title: Plant Physiology Volume: 176 Issue: 1 Pages: 199-207
  • DOI: 10.1104/pp.17.01522
  • Peer Reviewed / Open Access
• [Journal Article] Extension of oil biosynthesis during the mid-phase of seed development enhances oil content in Arabidopsis seeds. (2016)
  • Author(s): Kanai, M., Mano, S., Kondo, M., Hayashi, M., and Nishimura, M.
  • Journal Title: Plant Biotechnology Journal Volume: 14 Issue: 5 Pages: 1241-1250
  • DOI: 10.1111/pbi.12489
  • Peer Reviewed / Open Access
• [Journal Article] Sucrose production mediated by lipid metabolism suppresses physiological interaction of peroxisomes and oil bodies during germination of Arabidopsis thaliana. (2016)
  • Author(s): Cui, S., Hayashi, Y., Otomo, M., Mano, S., Oikawa, K., Hayashi, M. and Nishimura, M.
  • Journal Title: J. Biol. Chem. Volume: 291 Issue: 38 Pages: 19734-19745
  • DOI: 10.1074/jbc.m116.748814
  • Peer Reviewed
• [Journal Article] Physical interaction between peroxisomes and chloroplasts elucidated by in situ laser analysis (2015)
  • Author(s): Kazusato Oikawa, Shigeru Matsunaga, Shoji Mano, Maki Kondo, Kenji Yamada, Makoto Hayashi, Takatoshi Kagawa, Akeo Kadota, Wataru Sakamoto, Shoichi Higashi, Masakatsu Watanabe, Toshiaki Mitsui, Akinori Shigemasa, Takanori Iino, Yoichiroh Hosokawa & Mikio Nishimura
  • Journal Title: Nature Plants Volume: 1 Issue: 4 Pages: 15035-15035
  • DOI: 10.1038/nplants.2015.35
  • Peer Reviewed / Acknowledgement Compliant
• [Journal Article] Loss of Arabidopsis 5’-3’ exoribonuclease AtXRN4 function enhances heat stress tolerance of plants subjected to severe heat stress (2015)
  • Author(s): Anh Hai Nguyen, A. H., Matsui, A., Tanaka, M., Mizunashi, K., Nakaminami, K., Hayashi, M., Iida, K., Toyoda, T., Nguyen, D. V. and Seki, M.
  • Journal Title: Plant Cell Physiol. Volume: 56 Issue: 9 Pages: 1762-1772
  • DOI: 10.1093/pcp/pcv096
  • Peer Reviewed
• [Journal Article] A new temperature-insensitive allele of the Arabidopsis AXR6/CUL1 locus derived from a missense mutation in the C-terminal RBX1 binding region (2015)
  • Author(s): Mori, Y., Hayashi, M., Nishimura, M. and Yamamoto, K. T.
  • Journal Title: Plant Signal. Behav. Volume: 10 Issue: 11 Pages: e1078956-e1078956
  • DOI: 10.1080/15592324.2015.1078956
  • NAID: 120006360047
  • Peer Reviewed
• [Presentation] トリアシルグリセロールリパーゼSDP1はダイズの脂質含量と脂肪酸組成を制御する (2018)
  • Author(s): 金井雅武、山田哲也、林誠、真野昌二、西村幹夫
  • Organizer: 第５９回日本植物生理学会年会
• [Presentation] トリアシルグリセロールリパーゼSDP1の発現抑制はダイズの脂質含量と脂肪酸組成を改良する (2017)
  • Author(s): 金井雅武、山田哲也、林誠、真野昌二、西村幹夫
  • Organizer: ConBio2017（2017年度生命化学系学会合同年次大会）
• [Presentation] 葉酸によるデンプン蓄積の抑制メカニズム (2017)
  • Author(s): 林 誠、山本沙季
  • Organizer: 日本植物学会第８１回大会
• [Presentation] xrn4変異で誘導される遺伝子サイレンシングを抑制する遺伝子の探索 (2015)
  • Author(s): 斉藤美幸、林 誠
  • Organizer: 日本植物学会年会
  • Place of Presentation: 新潟
  • Year and Date: 2015-09-06
• [Book] New challenges in Seed biology - Basic and translational research driving seed technology (2016)
  • Author(s): Masatake Kanai, Shoji Mano, Makoto Hayashi and Mikio Nishimura
  • Total Pages: 201
  • Publisher: INTECH
• [Remarks] 長浜バイオ大学 植物環境細胞生化学研究室
  • URL: http://b-lab.nagahama-i-bio.ac.jp/?page_id=72
• [Remarks] 長浜バイオ大学 植物環境細胞生化学研究室
  • URL: http://b-lab.nagahama-i-bio.ac.jp/?page/id=72