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Study on seed germination regulated by peroxisomal membrane ABC transporter

Research Project

Project/Area Number 15K07117
Research Category

Grant-in-Aid for Scientific Research (C)

Allocation TypeMulti-year Fund
Section一般
Research Field Plant molecular biology/Plant physiology
Research InstitutionNagahama Institute of Bio-Science and Technology

Principal Investigator

Hayashi Makoto  長浜バイオ大学, バイオサイエンス学部, 教授 (50212155)

Project Period (FY) 2015-04-01 – 2018-03-31
Project Status Completed (Fiscal Year 2017)
Budget Amount *help
¥4,940,000 (Direct Cost: ¥3,800,000、Indirect Cost: ¥1,140,000)
Fiscal Year 2017: ¥1,950,000 (Direct Cost: ¥1,500,000、Indirect Cost: ¥450,000)
Fiscal Year 2016: ¥1,560,000 (Direct Cost: ¥1,200,000、Indirect Cost: ¥360,000)
Fiscal Year 2015: ¥1,430,000 (Direct Cost: ¥1,100,000、Indirect Cost: ¥330,000)
Keywordsペルオキシソーム / 種子発芽 / 貯蔵脂肪 / デンプン / 葉酸 / プラスチド / 発芽 / 脂質代謝 / 糖代謝 / グリオキシソーム / アブシジン酸 / ムシラゲ / 脂質分解
Outline of Final Research Achievements

PED3 is a peroxisomal membrane ABC transporter. Fatty acids released from seed reserved lipid in oil body are imported into peroxisome by PED3, and then converted into sucrose supplying essential energy for seed germination.
To identify genes regulating carbon metabolism during germination, we performed comprehensive isolation and molecular genetical analyses of ped3 suppressor mutants. Six mutants were so far identified and analyzed their phenotypes. One of the mutant was revealed that it had defect in plastidial folylpolyglutamate synthetase gene, and accumulated starch during germination in the dark.

Report

(4 results)
  • 2017 Annual Research Report   Final Research Report ( PDF )
  • 2016 Research-status Report
  • 2015 Research-status Report
  • Research Products

    (14 results)

All 2018 2017 2016 2015 Other

All Journal Article (7 results) (of which Peer Reviewed: 7 results,  Open Access: 3 results,  Acknowledgement Compliant: 1 results) Presentation (4 results) Book (1 results) Remarks (2 results)

  • [Journal Article] Plastidial Folate Prevents Starch Biosynthesis Triggered by Sugar Influx into Non-Photosynthetic Plastids of Arabidopsis2017

    • Author(s)
      Hayashi Makoto、Tanaka Mina、Yamamoto Saki、Nakagawa Taro、Kanai Masatake、Anegawa Aya、Ohnishi Miwa、Mimura Tetsuro、Nishimura Mikio
    • Journal Title

      Plant and Cell Physiology

      Volume: 58 Issue: 8 Pages: 1328-1338

    • DOI

      10.1093/pcp/pcx076

    • NAID

      120006473630

    • Related Report
      2017 Annual Research Report
    • Peer Reviewed / Open Access
  • [Journal Article] Membrane Dynamics and Multiple Functions of Oil Bodies in Seeds and Leaves2017

    • Author(s)
      Shimada Takashi L.、Hayashi Makoto、Hara-Nishimura Ikuko
    • Journal Title

      Plant Physiology

      Volume: 176 Issue: 1 Pages: 199-207

    • DOI

      10.1104/pp.17.01522

    • Related Report
      2017 Annual Research Report
    • Peer Reviewed / Open Access
  • [Journal Article] Extension of oil biosynthesis during the mid-phase of seed development enhances oil content in Arabidopsis seeds.2016

    • Author(s)
      Kanai, M., Mano, S., Kondo, M., Hayashi, M., and Nishimura, M.
    • Journal Title

      Plant Biotechnology Journal

      Volume: 14 Issue: 5 Pages: 1241-1250

    • DOI

      10.1111/pbi.12489

    • Related Report
      2016 Research-status Report
    • Peer Reviewed / Open Access
  • [Journal Article] Sucrose production mediated by lipid metabolism suppresses physiological interaction of peroxisomes and oil bodies during germination of Arabidopsis thaliana.2016

    • Author(s)
      Cui, S., Hayashi, Y., Otomo, M., Mano, S., Oikawa, K., Hayashi, M. and Nishimura, M.
    • Journal Title

      J. Biol. Chem.

      Volume: 291 Issue: 38 Pages: 19734-19745

    • DOI

      10.1074/jbc.m116.748814

    • Related Report
      2016 Research-status Report
    • Peer Reviewed
  • [Journal Article] Physical interaction between peroxisomes and chloroplasts elucidated by in situ laser analysis2015

    • Author(s)
      Kazusato Oikawa, Shigeru Matsunaga, Shoji Mano, Maki Kondo, Kenji Yamada, Makoto Hayashi, Takatoshi Kagawa, Akeo Kadota, Wataru Sakamoto, Shoichi Higashi, Masakatsu Watanabe, Toshiaki Mitsui, Akinori Shigemasa, Takanori Iino, Yoichiroh Hosokawa & Mikio Nishimura
    • Journal Title

      Nature Plants

      Volume: 1 Issue: 4 Pages: 15035-15035

    • DOI

      10.1038/nplants.2015.35

    • Related Report
      2015 Research-status Report
    • Peer Reviewed / Acknowledgement Compliant
  • [Journal Article] Loss of Arabidopsis 5’-3’ exoribonuclease AtXRN4 function enhances heat stress tolerance of plants subjected to severe heat stress2015

    • Author(s)
      Anh Hai Nguyen, A. H., Matsui, A., Tanaka, M., Mizunashi, K., Nakaminami, K., Hayashi, M., Iida, K., Toyoda, T., Nguyen, D. V. and Seki, M.
    • Journal Title

      Plant Cell Physiol.

      Volume: 56 Issue: 9 Pages: 1762-1772

    • DOI

      10.1093/pcp/pcv096

    • Related Report
      2015 Research-status Report
    • Peer Reviewed
  • [Journal Article] A new temperature-insensitive allele of the Arabidopsis AXR6/CUL1 locus derived from a missense mutation in the C-terminal RBX1 binding region2015

    • Author(s)
      Mori, Y., Hayashi, M., Nishimura, M. and Yamamoto, K. T.
    • Journal Title

      Plant Signal. Behav.

      Volume: 10 Issue: 11 Pages: e1078956-e1078956

    • DOI

      10.1080/15592324.2015.1078956

    • NAID

      120006360047

    • Related Report
      2015 Research-status Report
    • Peer Reviewed
  • [Presentation] トリアシルグリセロールリパーゼSDP1はダイズの脂質含量と脂肪酸組成を制御する2018

    • Author(s)
      金井雅武、山田哲也、林誠、真野昌二、西村幹夫
    • Organizer
      第59回日本植物生理学会年会
    • Related Report
      2017 Annual Research Report
  • [Presentation] トリアシルグリセロールリパーゼSDP1の発現抑制はダイズの脂質含量と脂肪酸組成を改良する2017

    • Author(s)
      金井雅武、山田哲也、林誠、真野昌二、西村幹夫
    • Organizer
      ConBio2017(2017年度生命化学系学会合同年次大会)
    • Related Report
      2017 Annual Research Report
  • [Presentation] 葉酸によるデンプン蓄積の抑制メカニズム2017

    • Author(s)
      林 誠、山本沙季
    • Organizer
      日本植物学会第81回大会
    • Related Report
      2017 Annual Research Report
  • [Presentation] xrn4変異で誘導される遺伝子サイレンシングを抑制する遺伝子の探索2015

    • Author(s)
      斉藤美幸、林 誠
    • Organizer
      日本植物学会年会
    • Place of Presentation
      新潟
    • Year and Date
      2015-09-06
    • Related Report
      2015 Research-status Report
  • [Book] New challenges in Seed biology - Basic and translational research driving seed technology2016

    • Author(s)
      Masatake Kanai, Shoji Mano, Makoto Hayashi and Mikio Nishimura
    • Total Pages
      201
    • Publisher
      INTECH
    • Related Report
      2016 Research-status Report
  • [Remarks] 長浜バイオ大学 植物環境細胞生化学研究室

    • URL

      http://b-lab.nagahama-i-bio.ac.jp/?page_id=72

    • Related Report
      2017 Annual Research Report 2015 Research-status Report
  • [Remarks] 長浜バイオ大学 植物環境細胞生化学研究室

    • URL

      http://b-lab.nagahama-i-bio.ac.jp/?page/id=72

    • Related Report
      2016 Research-status Report

URL: 

Published: 2015-04-16   Modified: 2019-03-29  

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