Clarification of novel calcium regulatory system in primitive vertebrate, Japanese lamprey, Lethenteron japonicum

• Project/Area Number: 15K07126
• Research Category: Grant-in-Aid for Scientific Research (C)
• Allocation Type: Multi-year Fund
• Section: 一般
• Research Field: Morphology/Structure
• Research Institution: Kanazawa University
• Principal Investigator: Sekiguchi Toshio 金沢大学, 環日本海域環境研究センター, 助教 (40378568)
• Co-Investigator(Kenkyū-buntansha): 鈴木 信雄 金沢大学, 環日本海域環境研究センター, 教授 (60242476) ; 服部 淳彦 東京医科歯科大学, 教養部, 教授 (70183910)
• Co-Investigator(Renkei-kenkyūsha): Kuraku Shigehiro 国立研究開発法人理化学研究所, ライフサイエンス技術基盤研究センター, ユニットリーダー (40391940)
• Project Period (FY): 2015-04-01 – 2018-03-31
• Project Status: Completed (Fiscal Year 2017)
• Budget Amount: ¥5,070,000 (Direct Cost: ¥3,900,000、Indirect Cost: ¥1,170,000); Fiscal Year 2017: ¥1,950,000 (Direct Cost: ¥1,500,000、Indirect Cost: ¥450,000); Fiscal Year 2016: ¥1,430,000 (Direct Cost: ¥1,100,000、Indirect Cost: ¥330,000); Fiscal Year 2015: ¥1,690,000 (Direct Cost: ¥1,300,000、Indirect Cost: ¥390,000)
• Keywords: カルシトニン / ヤツメウナギ / 円口類 / 比較内分泌学 / calcitonin / lamprey / evolution / cyclostome / カワヤツメウナギ / カルシウム代謝

Outline of Final Research Achievements

Vertebrates possess bones and scales as calcium reservoirs. Blood calcium levels are regulated by hypocalcemic hormones including calcitonin and stanniocalcin and hypercalcemic hormones comprising parathyroid hormone and calcitriol(1,25-dihydroxyvitamin D3). We investigated the role of hormones involved in the regulation of blood calcium levels in the Japanese lamprey, Lethenteron japonicum, which has no bones and scales. Calcitonin and its receptor were identified. In addition, transfer of lamprey from freshwater to 1/3 seawater results in an increase in plasma calcitonin concentration and expression of its receptor mRNA in the gill. We also newly identified stanniocalcin.

Research Products

• [Journal Article] α-melanocyte-stimulating hormone promotes the bone resorption resulting from increased osteoblastic and osteoclastic activities in goldfish. (2018)
  • Author(s): Ishizu, H., Sekiguchi, T., Ikari, T., Kitamura, K., Kitani, Y., Endo, M., Urata, M., Kinoshita, Y., Hattori, A., Srivastav, A.K., Mishima, H., Mizusawa, K., Takahashi, A. and Suzuki, N.
  • Journal Title: Gen. and Comp. Endocrinol. Volume: 262 Pages: 99-105
  • Peer Reviewed / Int'l Joint Research
• [Journal Article] Sequencing and expression analysis of calcitonin receptor in the scales of goldfish (Carassius auratus). (2018)
  • Author(s): Ikari, T., Sekiguchi, T., Urata, M., Furusawa, Y., Ikegame, M., Kinoshita, Y., Kitamura, K., Nakabayashi, I., Horita, M., Tabuchi, Y., Hattori, A., Srivastav, A.K. and Suzuki, N.
  • Journal Title: Int. J. Zool. Inv. Volume: 4 Pages: 1-10
  • Peer Reviewed / Int'l Joint Research
• [Journal Article] Sodium fluoride influences calcium metabolism resulting from the suppression of osteoclasts in the scales of nibbler fish Girella punctate. (2017)
  • Author(s): Sato, M., Yachiguchi, K., Motohashi, K., Yaguchi, Y., Tabuchi, Y., Kitani, Y., Ikaria, T., Ogiso, S., Sekiguchi, T., Hai, T.N., Huong, D.T.T., Hoang, N.V., Urata, M., Mishima, H., Hattori, A. and Suzuki, N.
  • Journal Title: Fisheries Sci. Volume: 83 Pages: 543-550
  • Peer Reviewed / Int'l Joint Research
• [Journal Article] Sardine procalcitonin amino-terminal cleavage peptide has a different action from calcitonin and promotes osteoblastic activity in the scales of goldfish. (2017)
  • Author(s): Kase, Y., Ikari, T., Sekiguchi, T., Sato, M., Ogiso, S., Kawada, T., Matsubara, S., Satake, H., Sasayama, Y., Endo, M., Kitamura, K., Hattori, A., Watanabe, T.X., Maruyama, Y., Watanabe, Y., Funahashi, H., Kambegawa, A., and Suzuki, N.
  • Journal Title: Comp. Biochem. Physiol. Part A Volume: 211 Pages: 77-83
  • NAID: 120006336026
  • Peer Reviewed
• [Journal Article] Diethylstilbestrol administration inhibits theca cell androgen and granulosa cell estrogen production in immature rat ovary. (2017)
  • Author(s): Imamichi, Y., Sekiguchi, T., Kitano, T., Kajitani, T., Okada, R., Inaoka, Y., Miyamoto, K., Uwada, J., Islam Khan, M.R., Islam, M.T., Yuhki, K., Kashiwagi, H., Ushikubi, F., Suzuki, N., Taniguchi, T., and Yazawa, T.
  • Journal Title: Sci. Rep. Volume: 7 Pages: 8374-8374
  • Peer Reviewed / Open Access / Int'l Joint Research
• [Journal Article] Benz[a]anthracene decreases plasma calcium levels resulting from influence of scale osteoclastic and osteoblastic activities in goldfish. (2017)
  • Author(s): Suzuki, N., Nakano, J., Kawabe, K., Toriba, A., Hayakawa, K., Tang, N, Sekiguchi, T., Tabuchi, Y., Ikegame, M., Shimizu, N., Mishima, H., Hattori, A., Srivastav, A.K. and Kitani, Y.
  • Journal Title: Int. J. Zool. Inv. Volume: 3 Pages: 72-81
  • Peer Reviewed / Int'l Joint Research
• [Journal Article] Pollution of radiocesium and radiosilver in wharf roach (Ligia sp.) by the Fukushima Dai-ichi Nuclear Power Plant accident. (2017)
  • Author(s): Qiu, X., Undap, S.L., Honda, M., Sekiguchi, T., Suzuki, N., Shimasaki, Y. Ando, H., Sato-Okoshi, W., Wada, T., Sunobe, T., Takeda, S., Munehara, H., Yokoyama, H., Momoshima, N., Oshima, Y.
  • Journal Title: J. Radioanal. Nucl. Chem. Volume: 311 Issue: 1 Pages: 121-126
  • DOI: 10.1007/s10967-016-4879-1
  • Peer Reviewed / Open Access / Int'l Joint Research
• [Journal Article] <b>Effects of low-intensity pulsed ultrasound on osteoclasts: Analysis with goldfish scales as a model of </b><b>bone </b> (2017)
  • Author(s): Hanmoto T, Tabuchi Y, Ikegame M, Kondo T, Kitamura KI, Endo M, Kobayashi I, Mishima H, Sekiguchi T, Urata M, Seki A, Yano S, Hattori A, Suzuki N.
  • Journal Title: Biomedical Research Volume: 38 Issue: 1 Pages: 71-77
  • DOI: 10.2220/biomedres.38.71
  • NAID: 130005242919
  • ISSN: 0388-6107, 1880-313X
  • Peer Reviewed / Open Access / Acknowledgement Compliant
• [Journal Article] Identification and molecular characterization of the stanniocalcin family gene from the inshore hagfish, Eptatretus burgeri. (2017)
  • Author(s): Sekiguchi, T., Kuraku, S., Tatsumi, K., Shimasaki, Y., Oshima, Y. and Suzuki, N.
  • Journal Title: J. Fac. Agr., Kyushu Univ. Volume: 62 Pages: 93-98
  • NAID: 120006220587
  • Int'l Joint Research / Acknowledgement Compliant
• [Journal Article] Calcitonin-typical suppression of osteoclastic activity by amphioxus calcitonin superfamily peptides and insights into the evolutionary conservation and diversity of their structures. (2017)
  • Author(s): Sekiguchi, T., Shiraishi, A., Satake, H., Kuwasako, K., Takahashi, H., Sato, M., Urata, M., Wada, S., Endo, M., Ikari, T., Hattori, A., Srivastav, A.K. and Suzuki, N.
  • Journal Title: Gen. Comp. Endocrinol. Volume: 246 Pages: 294-300
  • DOI: 10.1016/j.ygcen.2017.01.004
  • NAID: 120005973614
  • Peer Reviewed / Open Access / Int'l Joint Research / Acknowledgement Compliant
• [Journal Article] Low-intensity pulsed ultrasound induces apoptosis in osteoclasts: Fish scales are a suitable model for the analysis of bone metabolism by ultrasound. (2016)
  • Author(s): Suzuki N, Hanmoto T, Yano S, Furusawa Y, Ikegame M, Tabuchi Y, Kondo T, Kitamura K, Endo M, Yamamoto T, Sekiguchi T, Urata M, Mikuni-Takagaki Y, Hattori A.
  • Journal Title: Comp. Biochem. Physiol. Part A Volume: 195 Pages: 26-31
  • DOI: 10.1016/j.cbpa.2016.01.022
  • NAID: 120005743920
  • Peer Reviewed / Open Access / Int'l Joint Research / Acknowledgement Compliant
• [Journal Article] Seawater polluted with highly concentrated polycyclic aromatic hydrocarbons suppresses osteoblastic activity in the scales of goldfish, Carassius auratus. (2016)
  • Author(s): Suzuki, N., Sato, M., Nassar, F. H., Abdel-gawad, F. Kh., Bassem, S.M., Yachiguchi, K., Tabuchi, Y., Endo, M., Sekiguchi, T., Urata, M., Hattori, A., Mishima, H., Shimasaki, Y., Oshima, Y., Hong, C.-S., Makino, F., Tang, N., Toriba, A. and Hayakawa, K
  • Journal Title: Zool. Sci. Volume: 33 Issue: 4 Pages: 407-413
  • DOI: 10.2108/zs150211
  • NAID: 120005851366
  • Peer Reviewed / Open Access / Int'l Joint Research / Acknowledgement Compliant
• [Journal Article] Inhibitory effects of two G protein-coupled receptor kinases on the cell surface expression and signaling of the human adrenomedullin receptor. (2016)
  • Author(s): Kuwasako, K., Sekiguchi, T., Nagata, S., Jiang, D., Hayashi, H., Murakami, M., Hattori, Y., Kitamura, K. and Kato, J.
  • Journal Title: Biochem. Biophys. Res. Commun. Volume: 470 Issue: 4 Pages: 894-899
  • DOI: 10.1016/j.bbrc.2016.01.138
  • Peer Reviewed
• [Journal Article] Evidence for conservation of the calcitonin superfamily and activity-regulating mechanisms in the basal chordate Branchiostoma floridae: insight into the molecular and functional evolution in chordates. (2016)
  • Author(s): Sekiguchi, T., Kuwasako, K., Ogasawara, M., Takahashi, H., Matsubara, S., Osugi, T., Muramatsu, I., Sasayama, Y., Suzuki, N., and Satake, H.
  • Journal Title: J. Biol. Chem. Volume: 291 Issue: 5 Pages: 2345-2356
  • DOI: 10.1074/jbc.m115.664003
  • Peer Reviewed / Open Access / Acknowledgement Compliant
• [Journal Article] Monohydroxylated polycyclic aromatic hydrocarbon influences spicule formation in the early development of sea urchins (Hemicentrotus pulcherrimus). (2015)
  • Author(s): Suzuki, N., Ogiso, S., Yachiguchi, K., Kawabe, K., Makino, F., Toriba, A., Kiyomoto, M., Sekiguchi, T., Tabuchi, Y., Kondo, T., Kitamura, K., Hong, C., Srivastav, A.K., Oshima, Y., Hattori, A., Hayakawa, K.
  • Journal Title: Comp Biochem Physiol C Toxicol Pharmacol. Volume: 171 Pages: 55-60
  • DOI: 10.1016/j.cbpc.2015.02.004
  • Peer Reviewed / Open Access / Acknowledgement Compliant
• [Journal Article] Novel tryptophan derivatives as potentially effective therapeutic drugs to treat bone diseases. (2015)
  • Author(s): Suzuki, N., Somei, M., Seki, A., Sekiguchi, T., Tabuchi, Y., Mishima, H., Kase, Y., Kaminishi, A., Yachiguchi, K., Kitamura, K., Oshima, Y., Hayakawa, K., Yano, S. and Hattori, A.
  • Journal Title: Am. J. Life Sci. Volume: 3 Pages: 31-38
  • Peer Reviewed
• [Presentation] Evolution of calcitonin gene-related peptide family from invertebrate to vertebrates. (2018)
  • Author(s): Sekiguchi, T.
  • Organizer: Biological Sciences Seminars, University of Auckland, Auckland, New Zealand
  • Invited
• [Presentation] 円口類カワヤツメ（Lethenteron japonicum）におけるカルシトニンの分子構造解析． (2017)
  • Author(s): 関口俊男、半本泰三、谷口詩穂、谷内口考治、鈴木信雄
  • Organizer: 日本動物学会第88 回富山大会
• [Presentation] Evolutionary aspect of calcium homeostasis in cyclostomes (2016)
  • Author(s): Sekiguchi, T.
  • Organizer: he 22nd International Congress of Zoology
  • Place of Presentation: Okinawa Convention Center
  • Year and Date: 2016-11-15
  • Int'l Joint Research / Invited
• [Presentation] 円口類カワヤツメにおけるカルシトニン及びカルシトニン受容体の分子構造解析． (2016)
  • Author(s): 関口俊男，半本泰三，谷口詩穂，谷内口孝治，鈴木信雄
  • Organizer: 平成28年度動物学会中部支部大会
  • Place of Presentation: 静岡大学
  • Year and Date: 2016-09-10
• [Presentation] Localization analysis of cionin and its receptors in the ascidian, Ciona intestinalis. (2015)
  • Author(s): Taniguchi, S., Ogasawara, M., Satake, H., Suzuki, N. and Sekiguchi, T.
  • Organizer: CompBiol 2015 広島大会
  • Place of Presentation: JMSアステールプラザ，広島県
  • Year and Date: 2015-12-11
• [Presentation] 海洋深層水の魚類生理に対する影響 (2015)
  • Author(s): 五十里雄大，佐藤将之，小木曽正造，関口俊男，豊原知足，服部淳彦，神戸川明，朝比奈潔，鈴木信雄
  • Organizer: 平成27年度日本動物学会中部支部三重大会
  • Place of Presentation: 三重大学，三重県
  • Year and Date: 2015-11-28
• [Presentation] 魚類のカルシウム代謝に及ぼすフッ素の影響． (2015)
  • Author(s): 佐藤将之，半本泰三，谷内口孝治，関口俊男，田渕圭章，服部淳彦，鈴木信雄
  • Organizer: 平成27年度日本動物学会中部支部三重大会
  • Place of Presentation: 三重大学，三重県
  • Year and Date: 2015-11-28
• [Presentation] マイワシカルシトニンのクローニング． (2015)
  • Author(s): 加瀬陽一，佐藤将之，関口俊男，鈴木信雄
  • Organizer: 平成27年度日本動物学会中部支部三重大会
  • Place of Presentation: 三重大学，三重県
  • Year and Date: 2015-11-28
• [Presentation] 低出力超音波パルスの破骨細胞に対する作用． (2015)
  • Author(s): 半本泰三，田渕圭章，近藤 隆，北村敬一郎，関口俊男，高垣裕子，服部淳彦，鈴木信雄
  • Organizer: 平成27年度日本動物学会中部支部三重大会
  • Place of Presentation: 三重大学，三重県
  • Year and Date: 2015-11-28
• [Presentation] カタユウレイボヤにおけるCCK/gastrin familyペプチドおよびその受容体の局在解析． (2015)
  • Author(s): 谷口詩穂，小笠原道生，佐竹 炎，鈴木信雄，関口俊男
  • Organizer: 平成27年度日本動物学会中部支部三重大会
  • Place of Presentation: 三重大学，三重県
  • Year and Date: 2015-11-28
• [Presentation] ヤツメウナギにおけるカルシトニン及びカルシトニン受容体の分子構造解析． (2015)
  • Author(s): 関口俊男，半本泰三，谷口詩穂，谷内口孝治，鈴木信雄
  • Organizer: 平成27年度日本動物学会中部支部三重大会
  • Place of Presentation: 三重大学，三重県
  • Year and Date: 2015-11-28
• [Presentation] ヤツメウナギにおけるカルシトニンの構造及び生理作用に関する研究． (2015)
  • Author(s): 関口俊男，半本泰三，谷口詩穂，谷内口孝治，鈴木信雄
  • Organizer: 日本動物学会第86回新潟大会
  • Place of Presentation: 新潟コンベンションセンター朱鷺メッセ, 新潟県
  • Year and Date: 2015-09-17
• [Book] Toxicities of polycyclic aromatic hydrocarbons in fish and marine invertebrates. In “Polycyclic Aromatic Hydrocarbons: Environmental Behavior and Toxicity in East Asia” Ed. By K. Hayakawa (2018)
  • Author(s): Suzuki, N., Ikari, T., Sato, M., Toriba, A., Sekiguchi, T., Kitani, Y., Ogiso, S., Yachiguchi, K., Hattori, A., Oshima, Y. and Hayakawa, K.
  • Total Pages: 274
  • Publisher: Springer
• [Book] 第9章血液中のカルシウムを調節するしくみ，「ホメオスタシスと適応」 (2016)
  • Author(s): 鈴木信雄，関口俊男，服部淳彦
  • Total Pages: 227
  • Publisher: 裳華房
• [Book] Gastrin family. In “Handbook on Hormones”. Y. Takei, H. Ando, and K. Tsutsui. Eds. (2015)
  • Author(s): Sekiguchi, T.
  • Publisher: ELSEVIER, Oxford, United Kingdom
• [Book] Gastrin. In “Handbook on Hormones”. Y. Takei, H. Ando, and K. Tsutsui. Eds. (2015)
  • Author(s): Sekiguchi, T.
  • Publisher: ELSEVIER, Oxford, United Kingdom
• [Book] Cholecystokinin. In “Handbook on Hormones”. Y. Takei, H. Ando, and K. Tsutsui. Eds. (2015)
  • Author(s): Sekiguchi, T.
  • Publisher: ELSEVIER, Oxford, United Kingdom
• [Book] Caerulein. In “Handbook on Hormones”. Y. Takei, H. Ando, and K. Tsutsui. Eds. (2015)
  • Author(s): Sekiguchi, T.
  • Publisher: ELSEVIER, Oxford, United Kingdom