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Search for cell-cell interactions during ovarian development through inhibition of oocyte differentiation

Research Project

Project/Area Number 15K07127
Research Category

Grant-in-Aid for Scientific Research (C)

Allocation TypeMulti-year Fund
Section一般
Research Field Morphology/Structure
Research InstitutionNagoya University

Principal Investigator

KANAMORI AKIRA  名古屋大学, 理学研究科, 講師 (40324389)

Co-Investigator(Kenkyū-buntansha) 木下 政人  京都大学, 農学研究科, 助教 (60263125)
Project Period (FY) 2015-04-01 – 2019-03-31
Project Status Completed (Fiscal Year 2018)
Budget Amount *help
¥4,940,000 (Direct Cost: ¥3,800,000、Indirect Cost: ¥1,140,000)
Fiscal Year 2017: ¥1,560,000 (Direct Cost: ¥1,200,000、Indirect Cost: ¥360,000)
Fiscal Year 2016: ¥1,430,000 (Direct Cost: ¥1,100,000、Indirect Cost: ¥330,000)
Fiscal Year 2015: ¥1,950,000 (Direct Cost: ¥1,500,000、Indirect Cost: ¥450,000)
Keywords卵形成 / 転写因子 / 体細胞 / 生殖細胞 / 生殖巣 / 性分化 / ゲノム編集 / 減数分裂 / CRISPR/Cas9 / Pachytene check point / pachytene check point / 組織構築 / bHLH転写因子 / メダカ
Outline of Final Research Achievements

What molecular signals are working between somatic cells and germ cells during ovarian differentiation? In the present study, 1) we first knocked out oocyte-specific transcription factor, figα, by CRISPR/Cas9 system and verified that oocytes stopped entry into growth phase beyond pachytene. 2) The gonads, however, developed normally into ovaries suggesting that presence of growth phase oocytes are indispensable for normal ovarian development and further that ovarian somatic cells developed normally without growth phase oocytes.

Academic Significance and Societal Importance of the Research Achievements

我々ヒトを含む多細胞生物の発生では1つの細胞(受精卵)から多様な細胞系列が分化し、異なる細胞同士が集まり組織・器官を作り出す。これらの過程では細胞間の情報伝達により協調した分化過程が進むと考えられているが、その詳細な分子メカニズムには不明な場合が多い。生殖巣は比較的少数の細胞が、オスメスの二者択一を行うことから、器官形成時の細胞間情報伝達を研究する良いモデルと考えられる。本研究では生殖細胞の細胞自律的分化を阻害してやり、他の細胞群の分化への影響を調べることが可能であることを示せた。

Report

(6 results)
  • 2018 Annual Research Report   Final Research Report ( PDF )
  • 2017 Research-status Report
  • 2016 Research-status Report
  • 2015 Research-status Report
  • Products Report
  • Research Products

    (9 results)

All 2023 2017 2016 2015

All Journal Article (3 results) (of which Int'l Joint Research: 2 results,  Peer Reviewed: 3 results,  Open Access: 3 results) Presentation (6 results) (of which Int'l Joint Research: 2 results)

  • [Journal Article] Medaka factor in the germline alpha (figα) is indispensable for oogenesis but not for gonadal sex differentiation2023

    • Author(s)
      Katsuyasu Kamimura, Masato Kinoshita, Akira Kanamori
    • Journal Title

      Aquaculture and Fisheries

      Volume: 9 Issue: 3 Pages: 1-8

    • DOI

      10.1016/j.aaf.2023.02.006

    • Related Report
      Products Report
    • Peer Reviewed / Open Access
  • [Journal Article] Diversity, distribution, and significance of transposable elements in the genome of the only selfing hermaphroditic vertebrate Kryptolebias marmoratus.2017

    • Author(s)
      Rhee JS, Choi BS, Kim J, Kim BM, Lee YM, Kim IC, Kanamori A, Choi IY, Schartl M, Lee JS
    • Journal Title

      Scientific Reports

      Volume: 7 Issue: 1 Pages: 40121-40121

    • DOI

      10.1038/srep40121

    • Related Report
      2016 Research-status Report
    • Peer Reviewed / Open Access / Int'l Joint Research
  • [Journal Article] A Genetic Map for the Only Self-Fertilizing Vertebrate2016

    • Author(s)
      Akira Kanamori at al. (全部で13人)
    • Journal Title

      G3

      Volume: 6 Issue: 4 Pages: 1095-1106

    • DOI

      10.1534/g3.115.022699

    • Related Report
      2015 Research-status Report
    • Peer Reviewed / Open Access / Int'l Joint Research
  • [Presentation] A bHLH transcription factor, figα, is indispensable for oogenesis in medaka.2017

    • Author(s)
      Katsuyasu Kamimura, Masato Kinoshita, Hiroshi Hori, Akira Kanamori.
    • Organizer
      第23回小型魚類研究会
    • Related Report
      2017 Research-status Report
  • [Presentation] 自家受精魚のゲノム.2017

    • Author(s)
      金森 章
    • Organizer
      第12回Dmy研究会
    • Related Report
      2017 Research-status Report
  • [Presentation] The Genomes of the only self-fertilizing vertebrate2016

    • Author(s)
      Akira Kanamori, Jaebum Kim, Jae-Seong Lee, John H. Postlethwait, Yoshitaka Yoshitaka
    • Organizer
      The 22nd International Congress of Zoology and The 87th meeting of Zoological Society of Japan
    • Place of Presentation
      OIST&OCC 沖縄
    • Year and Date
      2016-11-14
    • Related Report
      2016 Research-status Report
    • Int'l Joint Research
  • [Presentation] Analyses of gonadal sex differentiation of medaka via knocking out figα, an oocyte-specific transcription factor2016

    • Author(s)
      Katsuyasu Kamimura, Masato Kinoshita, Hiroshi Hori, Akira Kanamori
    • Organizer
      The 22nd International Congress of Zoology and The 87th meeting of Zoological Society of Japan
    • Place of Presentation
      OIST&OCC 沖縄
    • Year and Date
      2016-11-14
    • Related Report
      2016 Research-status Report
    • Int'l Joint Research
  • [Presentation] 卵母細胞特異的転写因子figαノックアウトによるメダカ生殖巣性分化機構の解析2015

    • Author(s)
      神村 雄康,木下 政人,堀 寛,金森 章
    • Organizer
      第10回DMY研究会
    • Place of Presentation
      東大浜名湖水産実験所
    • Year and Date
      2015-11-28
    • Related Report
      2015 Research-status Report
  • [Presentation] 卵母細胞特異的転写因子figαノックアウトによるメダカ生殖巣性分化機構の解析2015

    • Author(s)
      神村 雄康,木下 政人,堀 寛,金森 章
    • Organizer
      日本動物学会 第八十六回大会
    • Place of Presentation
      朱鷺メッセ(新潟市)
    • Year and Date
      2015-09-17
    • Related Report
      2015 Research-status Report

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Published: 2015-04-16   Modified: 2025-03-27  

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