| Budget Amount *help |
¥4,940,000 (Direct Cost: ¥3,800,000、Indirect Cost: ¥1,140,000)
Fiscal Year 2017: ¥1,430,000 (Direct Cost: ¥1,100,000、Indirect Cost: ¥330,000)
Fiscal Year 2016: ¥1,430,000 (Direct Cost: ¥1,100,000、Indirect Cost: ¥330,000)
Fiscal Year 2015: ¥2,080,000 (Direct Cost: ¥1,600,000、Indirect Cost: ¥480,000)
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| Outline of Final Research Achievements |
We isolated Tmco5A gene using PCR-based subtraction technique. When expressed in COS7 cells, TMCO5A was distributed to endoplasmic reticulum-nuclear membrane (ER-NM) as a membrane-associated protein in the cells, while TMCO5A△C lacking the transmembrane region (TM) mislocalized and diffused throughout the cytoplasm. Double immunolabeling of isolated spermatids with the anti-TMCO5A and the anti-βtubulin antibodies showed that TMCO5A was always in close association with developing manchette microtubules, but not completely co-localized with them. On the other hand, we found that almost all TMCO5A was co-localized with SUN4, a LINC (Linker of nucleoskeleton and cytoskeleton) complex protein at the posterior part of nuclei in spermatids. These data suggested that TMCO5A is more closely located to the nuclei than manchette microtubules. It is likely that TMCO5A is involved in the process of spermiogenesis in which manchette microtubules are required.
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