| Project/Area Number |
15K07129
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| Research Category |
Grant-in-Aid for Scientific Research (C)
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| Allocation Type | Multi-year Fund |
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| Section | 一般 |
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| Research Field |
Morphology/Structure
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| Research Institution | Kumamoto Health Science University |
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Principal Investigator |
Abe Shin-ichi 熊本保健科学大学, 保健科学部, 教授 (90109637)
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| Co-Investigator(Renkei-kenkyūsha) |
KURAKU Shigehiro 特定国立研究開発法人理化学研究所生命機能科学研究センター, 分子配列比較解析ユニット, ユニットリーダー (40391940)
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| Research Collaborator |
ABE Kazuko 熊本保健科学大学, 保健科学部, 共同研究員
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| Project Period (FY) |
2015-04-01 – 2018-03-31
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| Project Status |
Completed (Fiscal Year 2017)
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| Budget Amount *help |
¥5,200,000 (Direct Cost: ¥4,000,000、Indirect Cost: ¥1,200,000)
Fiscal Year 2017: ¥1,040,000 (Direct Cost: ¥800,000、Indirect Cost: ¥240,000)
Fiscal Year 2016: ¥2,080,000 (Direct Cost: ¥1,600,000、Indirect Cost: ¥480,000)
Fiscal Year 2015: ¥2,080,000 (Direct Cost: ¥1,600,000、Indirect Cost: ¥480,000)
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| Keywords | マウス精巣 / ３次元培養 / 精細管 / 再構築 / KSR / ALK5 / Live Imaging / CD34+細胞 / p75+細胞 / 筋様細胞 / 再凝集培養 / Alk5 |
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| Outline of Final Research Achievements |
To address the testis formation mechanisms, we used 3-D re-aggregate cultures of testicular cells, and revealed following results: Ordered structures similar to interstitial tissues in vivo were reconstructed in re-aggregates consisting of PTMCs, p75+ and CD34+ cells. Interstitial cells were required for re-construction of tubule-like structures. Live cell imaging showed that dynamic cell movement and segregation occurred during the re-construction of tubule-like structures. ALK5i disturbed the proliferation of CD34+ cells, p75+ cells, PTMCs, and SCs, as well as movement of SCs and other types of cells. ALK5i compromised the re-construction of interstitial-like and tubule-like structures. Purified CD34+ cells cultured differentiated into p75+ cells and PTMCs, and ALK5i suppressed this differentiation. These results indicate that CD34+ cells and signaling through ALK5 play pivotal roles in the reconstruction of testicular structures.
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