| Project/Area Number |
15K07134
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| Research Category |
Grant-in-Aid for Scientific Research (C)
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| Allocation Type | Multi-year Fund |
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| Section | 一般 |
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| Research Field |
Morphology/Structure
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| Research Institution | Toho University |
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Principal Investigator |
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| Co-Investigator(Kenkyū-buntansha) |
小林 哲也 埼玉大学, 理工学研究科, 教授 (00195794)
菊山 榮 早稲田大学, 教育・総合科学学術院, 名誉教授 (20063638)
蓮沼 至 東邦大学, 理学部, 講師 (40434261)
岡田 令子 静岡大学, 理学部, 講師 (50386554)
中野 真樹 東邦大学, 理学部, 博士研究員 (20646195)
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| Project Period (FY) |
2015-04-01 – 2018-03-31
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| Project Status |
Completed (Fiscal Year 2017)
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| Budget Amount *help |
¥5,070,000 (Direct Cost: ¥3,900,000、Indirect Cost: ¥1,170,000)
Fiscal Year 2017: ¥1,040,000 (Direct Cost: ¥800,000、Indirect Cost: ¥240,000)
Fiscal Year 2016: ¥1,560,000 (Direct Cost: ¥1,200,000、Indirect Cost: ¥360,000)
Fiscal Year 2015: ¥2,470,000 (Direct Cost: ¥1,900,000、Indirect Cost: ¥570,000)
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| Keywords | ヒストン / 生体防御ペプチド / 細胞膜透過ペプチド / 細胞毒性 / 抗微生物活性 / リュウキュウアカガエル / 抗菌活性 / 抗菌ペプチド / 両生類 / Rana ulma / ヒストンH3 / 抗菌性 / 細胞膜透過性ペプチド / ヒストンH2B |
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| Outline of Final Research Achievements |
Recent reports have demonstrated the existence of extracellular histones. Extracellular histones have been shown to explicitly display antimicrobial properties and are known to be the major mediators of death during sepsis. In this study, we investigated antimicrobial and cytotoxic effects and the mode of action of histone H3 on various bacterial, fungal, and mammalian cells and identified functional region(s) within histone H3. The following results were obtained: (1) histone H3 exhibits growth-inhibitory effects against gram-negative and -positive bacteria and fungi through cell membrane destruction; (2) histone H3 exhibits strong cytotoxic effects on normal, immortal, and tumor cell lines through cell membrane destruction, but a lower cell density is required for immortal and tumor cells than for normal cells; (3) antimicrobial and cytotoxic functions are derived from different regions within histone H3.
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