Molecular basis of androgen dependent sex characteristics development and evolution
Project/Area Number |
15K07138
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Research Category |
Grant-in-Aid for Scientific Research (C)
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Allocation Type | Multi-year Fund |
Section | 一般 |
Research Field |
Morphology/Structure
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Research Institution | Kyushu University (2016-2017) Okazaki Research Facilities, National Institutes of Natural Sciences (2015) |
Principal Investigator |
Ogino Yukiko 九州大学, 農学研究院, 准教授 (00404343)
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Co-Investigator(Renkei-kenkyūsha) |
YAMADA GEN 和歌山県立医科大学, 先端医学研究所, 教授 (80174712)
WATANABE EIJI 基礎生物学研究所, 神経生理学研究室, 准教授 (30250252)
INOHAYA KEIJI 東京工業大学, 生命理工学研究所, 助教 (70302958)
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Project Period (FY) |
2015-04-01 – 2018-03-31
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Project Status |
Completed (Fiscal Year 2017)
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Budget Amount *help |
¥5,070,000 (Direct Cost: ¥3,900,000、Indirect Cost: ¥1,170,000)
Fiscal Year 2017: ¥1,430,000 (Direct Cost: ¥1,100,000、Indirect Cost: ¥330,000)
Fiscal Year 2016: ¥1,950,000 (Direct Cost: ¥1,500,000、Indirect Cost: ¥450,000)
Fiscal Year 2015: ¥1,690,000 (Direct Cost: ¥1,300,000、Indirect Cost: ¥390,000)
|
Keywords | アンドロゲン / 二次性徴 / ゲノム進化 / 性行動 / 真骨魚類 / ゲノム重複 |
Outline of Final Research Achievements |
Steroid hormone receptor genes are thought to have arisen from gene duplication (WGD). However, the molecular events which produce new protein functions have not been fully understood. Teleosts present a good model to investigate the evolutionary history of protein function after WGD, because the teleost-specific WGD (TSGD) resulted in a variety of duplicated genes that exist in modern fishes. We focused on the androgen receptor (AR) gene, since ARα and ARβ were generated in the TSGD. We identified the substitutions that led to changes in protein function between ARα and ARβ. One substitution located within ligand binding domain is sufficient for generating higher transactivation of ARα, which modifies the hydrogen bonds between ligand and AR. Analyses using AR mutant medaka revealed the different contribution of two AR subtypes for masculine phenotypes. Our findings would provide an historical explanation for the retention of the duplicated AR copies in euteleost genome.
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Report
(4 results)
Research Products
(33 results)
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[Journal Article] Establishment of estrogen receptor 1 (ESR1)-knockout medaka; ESR1 is dispensable for sexual development and reproduction in medaka, Oryzias latipes.2017
Author(s)
Tohyama S, Ogino Y, Lange A, Myosho T, Kobayashi T, Hirano Y, Yamada G, Sato T, Tatarazako N, Tyler CR, Iguchi T, Miyagawa S.
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Journal Title
Dev Growth Diff
Volume: 59
Issue: 6
Pages: 552-561
DOI
Related Report
Peer Reviewed / Int'l Joint Research
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[Journal Article] RNA-seq analysis of the gonadal transcriptome during Alligator mississippiensis temperature-dependent sex determination and differentiation.2016
Author(s)
Yatsu R, Miyagawa S, Kohno S, Parrott BB, Yamaguchi K, Ogino Y, Miyakawa H, Lowers RH, Shigenobu S, Guillette LJ Jr, Iguchi T.
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Journal Title
BMC Genomics
Volume: 17
Issue: 1
Pages: 77-77
DOI
Related Report
Peer Reviewed / Open Access / Int'l Joint Research / Acknowledgement Compliant
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[Journal Article] Characterization of evolutionary trend in squamate estrogen receptor sensitivity2016
Author(s)
Yatsu R, Katsu Y, Kohno S, Mizutani T, Ogino Y, Ohta Y, Myburgh J, van Wyk JH, Guillette Jr LJ, Miyagawa S, Iguchi T.
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Journal Title
Gen Comp Endocrinol
Volume: 238
Pages: 88-95
DOI
Related Report
Peer Reviewed / Int'l Joint Research / Acknowledgement Compliant
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[Journal Article] Evolution of estrogen receptors in ray-finned fish and their comparative responses to estrogenic substances2016
Author(s)
Tohyama S, Miyagawa S, Lange A, Ogino Y, Mizutani T, Ihara M, Tanaka H, Tatarazako N, Kobayashi T, Tyler CR, Iguchi T
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Journal Title
J Steroid Biochem Mol Biol
Volume: 158
Pages: 189-197
DOI
Related Report
Peer Reviewed / Int'l Joint Research
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[Journal Article] Identification and characterization of the androgen receptor from the American alligator, Alligator mississippiensis2015
Author(s)
Miyagawa S, Yatsu R, Kohno S, Doheny BM, Ogino Y, Ishibashi H, Katsu Y, Ohta Y, Guillette LJ Jr, Iguchi T
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Journal Title
Endocrinology
Volume: 156
Issue: 8
Pages: 2795-2806
DOI
Related Report
Peer Reviewed / Int'l Joint Research
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[Journal Article] Understanding the molecular basisi for differences in responses of fish estrogen receptor subtypes to environmental estrogens2015
Author(s)
Tohyama S, Miyagawa S, Lange A, Ogino Y, Mizutani T, Tatarazako N, Katsu Y, Ihara M, Tanaka H, Ishibashi H, Kobayashi T, Tyler CR, Iguchi T
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Journal Title
Environ Sci Technol
Volume: 49
Issue: 12
Pages: 7439-7447
DOI
Related Report
Peer Reviewed / Int'l Joint Research
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