Study on mechanisms linking epithelial field growth and periodic stripe formation

• Project/Area Number: 15K07139
• Research Category: Grant-in-Aid for Scientific Research (C)
• Allocation Type: Multi-year Fund
• Section: 一般
• Research Field: Morphology/Structure
• Research Institution: JT Biohistory Research Hall
• Principal Investigator: Oda Hiroki 株式会社生命誌研究館, その他部局等, 主任研究員 (50396222)
• Research Collaborator: Akiyama Yasuko (Oda Yasuko) ; Noda Akiko ; Hemmi Natsuki ; Iwasaki Sawa (Yokozawa Sawa)
• Project Period (FY): 2015-10-21 – 2019-03-31
• Project Status: Completed (Fiscal Year 2018)
• Budget Amount: ¥4,550,000 (Direct Cost: ¥3,500,000、Indirect Cost: ¥1,050,000); Fiscal Year 2017: ¥780,000 (Direct Cost: ¥600,000、Indirect Cost: ¥180,000); Fiscal Year 2016: ¥1,560,000 (Direct Cost: ¥1,200,000、Indirect Cost: ¥360,000); Fiscal Year 2015: ¥2,210,000 (Direct Cost: ¥1,700,000、Indirect Cost: ¥510,000)
• Keywords: パターン形成 / 体節形成 / 節足動物 / オオヒメグモ / モデル生物 / シグナル / 遺伝子発現 / ヘッジホッグ / 縞パターン / 細胞運動 / 上皮

Outline of Final Research Achievements

In this work, using the spider Parasteatoda tepidariorum embryonic field of epithelial cells where spatially periodic stripes of gene expression are formed, we intended to analyze the mechanisms that linked tissue dynamics and gene expression regulation. Using cell labeling and tracking, we performed a cell fate mapping of the stage 5 germ disc and showed that dynamic gene expression occurred in the cell populations while the stripe patterns being formed. Using timely fixed samples of sibling embryos, we conducted a quantitative reconstruction of stripe-forming processes varied depending on the body region in a common spatiotemporal framework. Moreover, we suggested that the armadillo/beta-catenin gene regulated gene expression dynamics associated with head segmentation in the spider.

Academic Significance and Societal Importance of the Research Achievements

動物胚の発生において、遺伝子発現パターンの時間発展は形態形成場の成長及び変形を伴う。しかしながら、“場”の成長・変形とパターン形成の緊密な関係を十分に理解することはできていない。この問題の一番のボトルネックは、細胞レベルの実験研究と再構成系による理論研究が両立しうる、実際の現象に基づくシンプルな実験解析系が存在しないことにある。本研究は、細胞挙動を追跡可能なシンプルなオオヒメグモ胚の形態形成場において、多様な縞パターン形成プロセスを共通の枠組みで定量的に再構成することに成功した。動物のパターン形成の研究において、実験研究と理論研究を両立しうる新たな実験系の構築を行った。

Research Products

• [Int'l Joint Research] Oxford Brookes University/University of Bristol(英国)
• [Int'l Joint Research] Georg-August- University(ドイツ)
• [Int'l Joint Research] University of Wisconsin-Madison(米国)
• [Int'l Joint Research] Oxford Brookes University(英国)
• [Int'l Joint Research] University Goettingen/Ernst Moritz Arndt University Greifswald/Georg-August-University(Germany)
• [Int'l Joint Research] Baylor College of Medicine/Washington and Lee University/Smithonian NMNH(米国)
• [Int'l Joint Research] Universite libre de Bruxelles(Belgium)
• [Int'l Joint Research] Aarhus University(Denmark)
• [Int'l Joint Research]
• [Journal Article] Experimental duplication of bilaterian body axes in spider embryos: Holm's organizer and self-regulation of embryonic fields. (2019)
  • Author(s): Oda, H., Iwasaki-Yokozawa, S., Usui, T., Akiyama-Oda, Y.
  • Journal Title: Dev. Genes Evol. Volume: - Issue: 2 Pages: 49-63
  • DOI: 10.1007/s00427-019-00631-x
  • Peer Reviewed / Open Access
• [Journal Article] Genome-scale embryonic developmental profile of gene expression in the common house spider Parasteatoda tepidariorum. (2018)
  • Author(s): Iwasaki-Yokozawa, S., Akiyama-Oda, Y., Oda, H.
  • Journal Title: Data Brief. Volume: 19 Pages: 865-867
  • DOI: 10.1016/j.dib.2018.05.106
  • Peer Reviewed / Open Access
• [Journal Article] Homeobox gene duplication and divergence in arachnids. (2018)
  • Author(s): Leite, D.J., Baudouin-Gonzalez, L., Iwasaki-Yokozawa, S., Lozano-Fernandez, J., Turetzek, N., Akiyama-Oda, Y., Prpic, N.M., Pisani, D., Oda, H., Sharma, P.P., McGregor, A.P.
  • Journal Title: Mol Biol Evol. Volume: - Issue: 9 Pages: 2240-2253
  • DOI: 10.1093/molbev/msy125
  • Peer Reviewed / Open Access / Int'l Joint Research
• [Journal Article] A quantitative study of the diversity of stripe-forming processes in an arthropod cell-based field undergoing axis formation and growth (2018)
  • Author(s): Natsuki Hemmi, Yasuko Akiyama-Oda, Koichi Fujimoto, Hiroki Oda
  • Journal Title: Developmental Biology Volume: 437 Issue: 2 Pages: 84-104
  • DOI: 10.1016/j.ydbio.2018.03.001
  • Peer Reviewed / Open Access
• [Journal Article] The house spider genome reveals an ancient whole-genome duplication during arachnid evolution (2017)
  • Author(s): Schwager, E. E., Sharma, P. P., Clarke, T., Leite, D. J., Wierschin, T., Pechmann, M., Akiyama-Oda, Y., Esposito, L., Bechsgaard, J.,(35 authors omitted), Bucher, G., Gibbs, R. A., Coddington, J., Oda, H., Stanke, M., Ayoub, N.A., Prpic, N. M., Flot, J. F., Posnien, N., Richards, S., McGregor, A.
  • Journal Title: BMC Biology Volume: 15 Issue: 1 Pages: 62-62
  • DOI: 10.1186/s12915-017-0399-x
  • Peer Reviewed / Open Access / Int'l Joint Research
• [Journal Article] Multi-color FISH facilitates analysis of cell-type diversification and developmental gene regulation in the Parasteatoda spider embryo (2016)
  • Author(s): Yasuko Akiyama-Oda and Hiroki Oda
  • Journal Title: Development, Growth and Differentiation Volume: 58 Issue: 2 Pages: 215-224
  • DOI: 10.1111/dgd.12263
  • Peer Reviewed / Open Access / Acknowledgement Compliant
• [Presentation] Segmentation dynamics and diversity in a cell-based field (2019)
  • Author(s): Hiroki Oda
  • Organizer: SpiderWeb meeting 2019 in London
  • Int'l Joint Research
• [Presentation] A spider model for studying cell-based pattern formation leading to the conserved stripes (2017)
  • Author(s): Hiroki Oda
  • Organizer: GZMB Colloquium Developmental Biology
  • Place of Presentation: ゲッティンゲン、ドイツ
  • Year and Date: 2017-03-20
  • Int'l Joint Research / Invited
• [Presentation] Stripe-forming waves of gene expression in a dynamic epithelial cell field of early spider embryo (2017)
  • Author(s): Hiroki Oda
  • Organizer: Joint meeting of the German and Japanese Societies of Developmental Biologists
  • Place of Presentation: キール、ドイツ
  • Year and Date: 2017-03-16
  • Int'l Joint Research / Invited
• [Presentation] A Hedgehog signaling network regulates the initiation of segmental oscillations in the early spider embryo (2017)
  • Author(s): Akiyama-Oda, Y., Iwasaki-Yokozawa, S., Oda, H.
  • Organizer: The 50th annual meeting of Japanese Society for Developmental Biology
  • Int'l Joint Research
• [Presentation] オオヒメグモの体節形成: 保存された縞パターンを作り出す遺伝子発現の波 (2017)
  • Author(s): 小田広樹
  • Organizer: 第５３回日本節足動物発生学会大会
  • Invited
• [Presentation] Cellular and quantitative characterization of three distinct processes of stripe formation in a growing epithelium of the spider Parasteatoda tepidariorum (2017)
  • Author(s): Oda, H., Hemmi, N., Fujimoto, K., Akiyama-Oda, Y.
  • Organizer: 18th International Congress of Developmental Biology
  • Int'l Joint Research
• [Presentation] A genome-wide study of Hedgehog signaling identifies a transcription factor gene mediating gene expression dynamics in the early spider embryo (2017)
  • Author(s): Akiyama-Oda, Y., Iwasaki-Yokozawa, S., Oda, H.
  • Organizer: 18th International Congress of Developmental Biology
• [Presentation] A spider model to study mechanisms behind the capacity for self-regulation in axis formation (2016)
  • Author(s): Sawa Iwasaki-Yokozawa, Yasuko Akiyama-Oda, Hiroki Oda
  • Organizer: Euro-Evo-Devo 第６回大会
  • Place of Presentation: ウプサラ、スウェーデン
  • Year and Date: 2016-07-27
  • Int'l Joint Research / Invited
• [Remarks] JT生命誌研究館ホームページ
  • URL: https://www.brh.co.jp
• [Remarks] JT生命誌研究館 BRH Data Resources
  • URL: https://www.brh2.jp
• [Remarks] Pt_spiderBASE
  • URL: https://www.e-celldev.jp/pt_spiderbase/
• [Remarks] Pt_spider Genome & Transcripts Data BASE Home
  • URL: https://www.e-celldev.jp/pt_spider2/
• [Remarks] JT生命誌研究館ホームページ
  • URL: http://www.brh.co.jp
• [Remarks] JT生命誌研究館 BRH Data Resources
  • URL: http://www.brh2.jp
• [Remarks] Pt_spiderBASE
  • URL: http://www.e-celldev.jp/pt_spiderbase/
• [Remarks] Pt_spider Genome & Transcripts Data BASE Home
  • URL: http://www.e-celldev.jp/pt_spider2/
• [Remarks] JT生命誌研究館 Data Resources
  • URL: http://www.brh2.jp
• [Remarks] Pt_spider Genome & Transcripts Data BASE
  • URL: http://www.e-celldev.jp/pt_spider2/index.html
• [Remarks] JT生命誌研究館
  • URL: http://www.brh.co.jp
• [Remarks] ハエとクモ、そしてヒトの祖先を知ろう（細胞•発生•進化研究室）
  • URL: http://www.brh.co.jp/research/lab04/
• [Remarks] Data Resources (JT Biohistory Research Hall)
  • URL: http://www.brh2.jp